Evaluation of pyrogallol-induced cytotoxicity in catalase-mutant Escherichia coli and mutagenicity in Salmonella typhimurium

Yoko Takemura, Da Hong Wang, Rani Sauriasari, Masako Horita, Ken Tsutsui, Kuniaki Sano, Noriyoshi Masuoka, Tomoko Takigawa, Jiro Takaki, Keiki Ogino

Research output: Contribution to journalArticle

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Abstract

We evaluated pyrogallol cytotoxicity using Escherichia coli strains that express mammalian catalase gene derived from catalase mutant mice (Cs b) and wild-type (Cs a), and pyrogallol mutagenicity by Ames test. Pyrogallol was more toxic to Cs b rather than to Cs a (p <0.05), while catalase, superoxide dismutase and ascorbic acid decrease the toxic effect. Pyrogallol also showed mutagenic effect (mutagenic index = 3.8 for 10 μmol pyrogallol/plate) while ascorbic acid (19.4% reduction, p <0.001) and naringin (35.1% reduction, p <0.001) played a protective role against it. Pyrogallol cytotoxicity and mutagenicity seem to be attributable, at least in part, to reactive oxygen species formation. This study also suggests that newly established catalase mutant E. coli is probably useful in hazard identification of oxidative chemicals.

Original languageEnglish
Pages (from-to)347-350
Number of pages4
JournalBulletin of Environmental Contamination and Toxicology
Volume84
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Pyrogallol
Salmonella
Ascorbic acid
Salmonella typhimurium
Cytotoxicity
Catalase
Escherichia coli
Hazards
Genes
Poisons
Ascorbic Acid
Oxygen
Mutagenicity Tests
Superoxide Dismutase
Reactive Oxygen Species

Keywords

  • Catalase-mutant Escherichia coli
  • Cytotoxicity
  • Mutagenicity
  • Pyrogallol

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Toxicology

Cite this

Evaluation of pyrogallol-induced cytotoxicity in catalase-mutant Escherichia coli and mutagenicity in Salmonella typhimurium. / Takemura, Yoko; Wang, Da Hong; Sauriasari, Rani; Horita, Masako; Tsutsui, Ken; Sano, Kuniaki; Masuoka, Noriyoshi; Takigawa, Tomoko; Takaki, Jiro; Ogino, Keiki.

In: Bulletin of Environmental Contamination and Toxicology, Vol. 84, No. 3, 03.2010, p. 347-350.

Research output: Contribution to journalArticle

Takemura, Yoko ; Wang, Da Hong ; Sauriasari, Rani ; Horita, Masako ; Tsutsui, Ken ; Sano, Kuniaki ; Masuoka, Noriyoshi ; Takigawa, Tomoko ; Takaki, Jiro ; Ogino, Keiki. / Evaluation of pyrogallol-induced cytotoxicity in catalase-mutant Escherichia coli and mutagenicity in Salmonella typhimurium. In: Bulletin of Environmental Contamination and Toxicology. 2010 ; Vol. 84, No. 3. pp. 347-350.
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