Abstract
To determine whether grafted young periosteum can induce new bone formation in elderly patients, this preliminary study evaluated cell proliferation and differentiation in xenogeneic periosteal grafts in old rats radiographically, histologically, and immunohistochemically. Periosteum harvested from the tibia of young Japanese white rabbits were grafted into old Sprague-Dawley rats with or without administration of 1.0 mg per kilogram per day immunosuppressant FK506. Autogenous old periosteal tissue grafts were also evaluated as a control. Grafted tissue was extirpated after 7, 14, 21, and 45 days. In the xenogeneic group, proliferative cell nuclear antigen-positive cells were observed 7 days after surgery, which differentiated into chondroblasts with bone morphogenetic protein-2 expression and finally formed cartilage by 14 days. Endochondral ossification was observed at 21 days, and bone replacement was completed by 45 days. No osteogenic cell activity was observed in the two other groups. Xenogeneic young periosteum thus maintained its osteogenic/chondrogenic potentiality in older rats.
| Original language | English |
|---|---|
| Pages (from-to) | 539-545 |
| Number of pages | 7 |
| Journal | Annals of Plastic Surgery |
| Volume | 48 |
| Issue number | 5 |
| Publication status | Published - 2002 |
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Evaluation of osteogenic/chondrogenic cellular proliferation and differentiation in the xenogeneic periosteal graft. / Ueno, Takaaki; Kagawa, Toshimasa; Fukunaga, Joji; Mizukawa, Nobuyoshi; Sugahara, Toshio; Yamamoto, Toshio.
In: Annals of Plastic Surgery, Vol. 48, No. 5, 2002, p. 539-545.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of osteogenic/chondrogenic cellular proliferation and differentiation in the xenogeneic periosteal graft
AU - Ueno, Takaaki
AU - Kagawa, Toshimasa
AU - Fukunaga, Joji
AU - Mizukawa, Nobuyoshi
AU - Sugahara, Toshio
AU - Yamamoto, Toshio
PY - 2002
Y1 - 2002
N2 - To determine whether grafted young periosteum can induce new bone formation in elderly patients, this preliminary study evaluated cell proliferation and differentiation in xenogeneic periosteal grafts in old rats radiographically, histologically, and immunohistochemically. Periosteum harvested from the tibia of young Japanese white rabbits were grafted into old Sprague-Dawley rats with or without administration of 1.0 mg per kilogram per day immunosuppressant FK506. Autogenous old periosteal tissue grafts were also evaluated as a control. Grafted tissue was extirpated after 7, 14, 21, and 45 days. In the xenogeneic group, proliferative cell nuclear antigen-positive cells were observed 7 days after surgery, which differentiated into chondroblasts with bone morphogenetic protein-2 expression and finally formed cartilage by 14 days. Endochondral ossification was observed at 21 days, and bone replacement was completed by 45 days. No osteogenic cell activity was observed in the two other groups. Xenogeneic young periosteum thus maintained its osteogenic/chondrogenic potentiality in older rats.
AB - To determine whether grafted young periosteum can induce new bone formation in elderly patients, this preliminary study evaluated cell proliferation and differentiation in xenogeneic periosteal grafts in old rats radiographically, histologically, and immunohistochemically. Periosteum harvested from the tibia of young Japanese white rabbits were grafted into old Sprague-Dawley rats with or without administration of 1.0 mg per kilogram per day immunosuppressant FK506. Autogenous old periosteal tissue grafts were also evaluated as a control. Grafted tissue was extirpated after 7, 14, 21, and 45 days. In the xenogeneic group, proliferative cell nuclear antigen-positive cells were observed 7 days after surgery, which differentiated into chondroblasts with bone morphogenetic protein-2 expression and finally formed cartilage by 14 days. Endochondral ossification was observed at 21 days, and bone replacement was completed by 45 days. No osteogenic cell activity was observed in the two other groups. Xenogeneic young periosteum thus maintained its osteogenic/chondrogenic potentiality in older rats.
UR - http://www.scopus.com/inward/record.url?scp=0036253612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036253612&partnerID=8YFLogxK
M3 - Article
C2 - 11981197
AN - SCOPUS:0036253612
VL - 48
SP - 539
EP - 545
JO - Annals of Plastic Surgery
JF - Annals of Plastic Surgery
SN - 0148-7043
IS - 5
ER -