Evaluation of osteogenic/chondrogenic cellular proliferation and differentiation in the xenogeneic periosteal graft

Takaaki Ueno, Toshimasa Kagawa, Joji Fukunaga, Nobuyoshi Mizukawa, Toshio Sugahara, Toshio Yamamoto

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Abstract

To determine whether grafted young periosteum can induce new bone formation in elderly patients, this preliminary study evaluated cell proliferation and differentiation in xenogeneic periosteal grafts in old rats radiographically, histologically, and immunohistochemically. Periosteum harvested from the tibia of young Japanese white rabbits were grafted into old Sprague-Dawley rats with or without administration of 1.0 mg per kilogram per day immunosuppressant FK506. Autogenous old periosteal tissue grafts were also evaluated as a control. Grafted tissue was extirpated after 7, 14, 21, and 45 days. In the xenogeneic group, proliferative cell nuclear antigen-positive cells were observed 7 days after surgery, which differentiated into chondroblasts with bone morphogenetic protein-2 expression and finally formed cartilage by 14 days. Endochondral ossification was observed at 21 days, and bone replacement was completed by 45 days. No osteogenic cell activity was observed in the two other groups. Xenogeneic young periosteum thus maintained its osteogenic/chondrogenic potentiality in older rats.

Original languageEnglish
Pages (from-to)539-545
Number of pages7
JournalAnnals of Plastic Surgery
Volume48
Issue number5
Publication statusPublished - 2002

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Periosteum
Cell Proliferation
Transplants
Osteogenesis
Bone Morphogenetic Protein 2
Nuclear Antigens
Tacrolimus
Immunosuppressive Agents
Chondrocytes
Tibia
Ambulatory Surgical Procedures
Cartilage
Sprague Dawley Rats
Cell Differentiation
Rabbits
Bone and Bones

ASJC Scopus subject areas

  • Surgery

Cite this

Evaluation of osteogenic/chondrogenic cellular proliferation and differentiation in the xenogeneic periosteal graft. / Ueno, Takaaki; Kagawa, Toshimasa; Fukunaga, Joji; Mizukawa, Nobuyoshi; Sugahara, Toshio; Yamamoto, Toshio.

In: Annals of Plastic Surgery, Vol. 48, No. 5, 2002, p. 539-545.

Research output: Contribution to journalArticle

Ueno, Takaaki ; Kagawa, Toshimasa ; Fukunaga, Joji ; Mizukawa, Nobuyoshi ; Sugahara, Toshio ; Yamamoto, Toshio. / Evaluation of osteogenic/chondrogenic cellular proliferation and differentiation in the xenogeneic periosteal graft. In: Annals of Plastic Surgery. 2002 ; Vol. 48, No. 5. pp. 539-545.
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