Evaluation of intracerebral grafting of dopamine-secreting PC12 cells into allogeneic and xenogeneic brain

Takeshi Ono, Isao Date, Takashi Imaoka, Tetsuro Shingo, Tomohisa Furuta, Shoji Asari, Takashi Ohmoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The PC12 pheochromocytoma tumor cell line is derived from a rat adrenal medullary tumor and secretes dopamine. We have previously reported that grafted microencapsulated PC12 cells using agarose and poly(styrene sulfonic acid) survived in the xenogeneic brain without immunosuppression. To investigate whether unencapsulated PC12 cells form a tumor and how they provoke immunological reaction, PC12 cell suspension was implanted into the striatum of Sprague-Dawley rat (allogeneic graft) or guinea pig (xenogeneic graft) and histological analysis using Nissl stain and immunocytochemical analysis using antityrosine hydroxylase (TH) antibody were performed 1, 2, and 4 wk after transplantation. Host animals were not immunosuppressed. PC12 cells formed a mass 1 and 2 wk after transplantation both in allogeneic and xenogeneic brain. These grafted PC12 cells were immunoreactive to anti-TH antibody. Four weeks after transplantation, however, grafted PC12 cells in the allogeneic brain were only found within the restricted area near the site of implantation. In the xenogeneic brain, only the trace of grafted PC12 cells were found around the site of implantation 4 wk after transplantation. In both allogeneic and xenogeneic animals, a number of lymphocytes were found in and around the grafts at all period investigated. These findings indicate that PC12 cells could survive in the allogeneic or xenogeneic brain for 2 wk and were ultimately rejected by immunological reaction by 4 wk after transplantation. Implantation of encapsulated PC12 cells in the allogeneic or xenogeneic brain is considered a safe and effective method for delivering dopamine into the brain because PC12 cells will not form a tumor in the long- term even if capsules are damaged in some reason.

Original languageEnglish
Pages (from-to)511-513
Number of pages3
JournalCell Transplantation
Volume6
Issue number5
DOIs
Publication statusPublished - Sep 1997

Fingerprint

PC12 Cells
Dopamine
Brain
Tumors
Grafts
Transplantation
Antibodies
Rats
Animals
Transplants
Lymphocytes
Brain Stem Neoplasms
Styrene
Sulfonic Acids
Cells
Lymphocyte Count
Mixed Function Oxygenases
Tumor Cell Line
Sepharose
Immunosuppression

Keywords

  • Encapsulation
  • Immunological rejection
  • Neural transplantation
  • Parkinson's disease
  • PC12 cells

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Evaluation of intracerebral grafting of dopamine-secreting PC12 cells into allogeneic and xenogeneic brain. / Ono, Takeshi; Date, Isao; Imaoka, Takashi; Shingo, Tetsuro; Furuta, Tomohisa; Asari, Shoji; Ohmoto, Takashi.

In: Cell Transplantation, Vol. 6, No. 5, 09.1997, p. 511-513.

Research output: Contribution to journalArticle

Ono, Takeshi ; Date, Isao ; Imaoka, Takashi ; Shingo, Tetsuro ; Furuta, Tomohisa ; Asari, Shoji ; Ohmoto, Takashi. / Evaluation of intracerebral grafting of dopamine-secreting PC12 cells into allogeneic and xenogeneic brain. In: Cell Transplantation. 1997 ; Vol. 6, No. 5. pp. 511-513.
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