TY - JOUR
T1 - Evaluation of Honeycomb Scaffold Combined with KUSA/A1 Cells for Tissue Engineering
AU - Takagi, Tohru
AU - Rodriguez, Andrea Paola
AU - Borkosky, Silvia Susana
AU - Rui, Kan
AU - Haillong, Hu
AU - Nagatsuka, Hitoshi
AU - Nagai, Noriyuki
AU - Oida, Shinichiro
AU - Komuro, Miki
PY - 2005/1
Y1 - 2005/1
N2 - We evaluated the efficacy of honeycomb scaffold combined with KUSA/A1 cells in vivo. The transplants were subjected to radiographical, histological and immunohistochemical examinations after 2 and 4 weeks of implantation. KUSA/A1 cells alone showed small nests of bone formation. Whereas, KUSA/A1-Atelocollagen revealed abundant new bone formation. We also determined the immunolocalization of type I collagen, CD34, Osteocalcin, and PCNA in this newly formed bone. Our results indicated that collagen scaffold plays an important role allowing vessel formation and cell anchorage especially through the proliferation and differentiation process in a confined space. This study can possible enhances existing therapeutic applications.
AB - We evaluated the efficacy of honeycomb scaffold combined with KUSA/A1 cells in vivo. The transplants were subjected to radiographical, histological and immunohistochemical examinations after 2 and 4 weeks of implantation. KUSA/A1 cells alone showed small nests of bone formation. Whereas, KUSA/A1-Atelocollagen revealed abundant new bone formation. We also determined the immunolocalization of type I collagen, CD34, Osteocalcin, and PCNA in this newly formed bone. Our results indicated that collagen scaffold plays an important role allowing vessel formation and cell anchorage especially through the proliferation and differentiation process in a confined space. This study can possible enhances existing therapeutic applications.
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U2 - 10.2485/jhtb.14.223
DO - 10.2485/jhtb.14.223
M3 - Article
AN - SCOPUS:85024715820
VL - 14
SP - 223
EP - 224
JO - Journal of Hard Tissue Biology
JF - Journal of Hard Tissue Biology
SN - 1341-7649
IS - 2
ER -