Evaluation of Honeycomb Scaffold Combined with KUSA/A1 Cells for Tissue Engineering

Tohru Takagi; Andrea Paola Rodriguez; Silvia Susana Borkosky; Kan Rui; Hu Haillong; Hitoshi Nagatsuka; Noriyuki Nagai; Shinichiro Oida; Miki Komuro

doi:10.2485/jhtb.14.223

Evaluation of Honeycomb Scaffold Combined with KUSA/A1 Cells for Tissue Engineering

Tohru Takagi, Andrea Paola Rodriguez, Silvia Susana Borkosky, Kan Rui, Hu Haillong, Hitoshi Nagatsuka, Noriyuki Nagai, Shinichiro Oida, Miki Komuro

Research output: Contribution to journal › Article › peer-review

Abstract

We evaluated the efficacy of honeycomb scaffold combined with KUSA/A1 cells in vivo. The transplants were subjected to radiographical, histological and immunohistochemical examinations after 2 and 4 weeks of implantation. KUSA/A1 cells alone showed small nests of bone formation. Whereas, KUSA/A1-Atelocollagen revealed abundant new bone formation. We also determined the immunolocalization of type I collagen, CD34, Osteocalcin, and PCNA in this newly formed bone. Our results indicated that collagen scaffold plays an important role allowing vessel formation and cell anchorage especially through the proliferation and differentiation process in a confined space. This study can possible enhances existing therapeutic applications.

Original language	English
Pages (from-to)	223-224
Number of pages	2
Journal	Journal of Hard Tissue Biology
Volume	14
Issue number	2
DOIs	https://doi.org/10.2485/jhtb.14.223
Publication status	Published - Jan 2005
Externally published	Yes

ASJC Scopus subject areas

Medicine (miscellaneous)
Biochemistry
Biomaterials
Orthopedics and Sports Medicine
Dentistry(all)
Cell Biology

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10.2485/jhtb.14.223

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Evaluation of Honeycomb Scaffold Combined with KUSA/A1 Cells for Tissue Engineering. / Takagi, Tohru; Rodriguez, Andrea Paola; Borkosky, Silvia Susana; Rui, Kan; Haillong, Hu; Nagatsuka, Hitoshi; Nagai, Noriyuki; Oida, Shinichiro; Komuro, Miki.

In: Journal of Hard Tissue Biology, Vol. 14, No. 2, 01.2005, p. 223-224.

Research output: Contribution to journal › Article › peer-review

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abstract = "We evaluated the efficacy of honeycomb scaffold combined with KUSA/A1 cells in vivo. The transplants were subjected to radiographical, histological and immunohistochemical examinations after 2 and 4 weeks of implantation. KUSA/A1 cells alone showed small nests of bone formation. Whereas, KUSA/A1-Atelocollagen revealed abundant new bone formation. We also determined the immunolocalization of type I collagen, CD34, Osteocalcin, and PCNA in this newly formed bone. Our results indicated that collagen scaffold plays an important role allowing vessel formation and cell anchorage especially through the proliferation and differentiation process in a confined space. This study can possible enhances existing therapeutic applications.",

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AU - Takagi, Tohru

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AU - Haillong, Hu

AU - Nagatsuka, Hitoshi

AU - Nagai, Noriyuki

AU - Oida, Shinichiro

AU - Komuro, Miki

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N2 - We evaluated the efficacy of honeycomb scaffold combined with KUSA/A1 cells in vivo. The transplants were subjected to radiographical, histological and immunohistochemical examinations after 2 and 4 weeks of implantation. KUSA/A1 cells alone showed small nests of bone formation. Whereas, KUSA/A1-Atelocollagen revealed abundant new bone formation. We also determined the immunolocalization of type I collagen, CD34, Osteocalcin, and PCNA in this newly formed bone. Our results indicated that collagen scaffold plays an important role allowing vessel formation and cell anchorage especially through the proliferation and differentiation process in a confined space. This study can possible enhances existing therapeutic applications.

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Evaluation of Honeycomb Scaffold Combined with KUSA/A1 Cells for Tissue Engineering

Abstract

ASJC Scopus subject areas

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