Abstract
Background: The main purpose of this study was to evaluate an HPLC method for LDL-cholesterol determination in the presence of abnormal lipoproteins. Methods: We compared LDL-cholesterol levels obtained by HPLC (HPLC-LDL), Friedewald (F-LDL), and β-quantification (BQ-LDL) methods on 47 healthy volunteers and 50 outpatients with lipid disorders, including apolipoprotein E2/2 phenotype, cholesteryl ester transfer protein deficiency and lipoprotein lipase deficiency. Results: For the control group (n = 50), the HPLC-LDL and the F-LDL correlated highly with the BQ-LDL (r = 0.984 and 0.969, respectively), but the HPLC-LDL was lower than the BQ-LDL (mean bias: - 4.0%, P <0.001). For the subjects with lipoprotein abnormalities, significant biases were found in HPLC-LDL for the hypertriglyceridemia (+ 25%, n = 17, P <0.01), the hyper HDL (- 15.2%, n = 10, P <0.01) and the hyper lipoprotein(a) groups (- 13.4%, n = 12, P <0.001). The F-LDL was significantly higher than the BQ-LDL in the apolipoprotein E2/2 subjects (+ 92%, n = 8, P <0.001), but not significantly different in other subjects with triglycerides <4000 mg/l. Conclusions: There were several discrepancies in LDL-cholesterol levels determined by the HPLC and the BQ methods in samples with lipoprotein abnormalities. However, the HPLC method can be useful and informative for analysis of abnormal lipoproteins.
| Original language | English |
|---|---|
| Pages (from-to) | 62-67 |
| Number of pages | 6 |
| Journal | Clinica Chimica Acta |
| Volume | 395 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Sep 2008 |
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Keywords
- Dysbetalipoproteinemia
- HPLC
- LDL-cholesterol determination
- Reference method
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
Cite this
Evaluation of an HPLC method for LDL-cholesterol determination in patients with various lipoprotein abnormalities in comparison with beta-quantification. / Okazaki, Mitsuyo; Usui, Shinichi; Nakamura, Masakazu; Yamashita, Shizuya.
In: Clinica Chimica Acta, Vol. 395, No. 1-2, 09.2008, p. 62-67.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of an HPLC method for LDL-cholesterol determination in patients with various lipoprotein abnormalities in comparison with beta-quantification
AU - Okazaki, Mitsuyo
AU - Usui, Shinichi
AU - Nakamura, Masakazu
AU - Yamashita, Shizuya
PY - 2008/9
Y1 - 2008/9
N2 - Background: The main purpose of this study was to evaluate an HPLC method for LDL-cholesterol determination in the presence of abnormal lipoproteins. Methods: We compared LDL-cholesterol levels obtained by HPLC (HPLC-LDL), Friedewald (F-LDL), and β-quantification (BQ-LDL) methods on 47 healthy volunteers and 50 outpatients with lipid disorders, including apolipoprotein E2/2 phenotype, cholesteryl ester transfer protein deficiency and lipoprotein lipase deficiency. Results: For the control group (n = 50), the HPLC-LDL and the F-LDL correlated highly with the BQ-LDL (r = 0.984 and 0.969, respectively), but the HPLC-LDL was lower than the BQ-LDL (mean bias: - 4.0%, P <0.001). For the subjects with lipoprotein abnormalities, significant biases were found in HPLC-LDL for the hypertriglyceridemia (+ 25%, n = 17, P <0.01), the hyper HDL (- 15.2%, n = 10, P <0.01) and the hyper lipoprotein(a) groups (- 13.4%, n = 12, P <0.001). The F-LDL was significantly higher than the BQ-LDL in the apolipoprotein E2/2 subjects (+ 92%, n = 8, P <0.001), but not significantly different in other subjects with triglycerides <4000 mg/l. Conclusions: There were several discrepancies in LDL-cholesterol levels determined by the HPLC and the BQ methods in samples with lipoprotein abnormalities. However, the HPLC method can be useful and informative for analysis of abnormal lipoproteins.
AB - Background: The main purpose of this study was to evaluate an HPLC method for LDL-cholesterol determination in the presence of abnormal lipoproteins. Methods: We compared LDL-cholesterol levels obtained by HPLC (HPLC-LDL), Friedewald (F-LDL), and β-quantification (BQ-LDL) methods on 47 healthy volunteers and 50 outpatients with lipid disorders, including apolipoprotein E2/2 phenotype, cholesteryl ester transfer protein deficiency and lipoprotein lipase deficiency. Results: For the control group (n = 50), the HPLC-LDL and the F-LDL correlated highly with the BQ-LDL (r = 0.984 and 0.969, respectively), but the HPLC-LDL was lower than the BQ-LDL (mean bias: - 4.0%, P <0.001). For the subjects with lipoprotein abnormalities, significant biases were found in HPLC-LDL for the hypertriglyceridemia (+ 25%, n = 17, P <0.01), the hyper HDL (- 15.2%, n = 10, P <0.01) and the hyper lipoprotein(a) groups (- 13.4%, n = 12, P <0.001). The F-LDL was significantly higher than the BQ-LDL in the apolipoprotein E2/2 subjects (+ 92%, n = 8, P <0.001), but not significantly different in other subjects with triglycerides <4000 mg/l. Conclusions: There were several discrepancies in LDL-cholesterol levels determined by the HPLC and the BQ methods in samples with lipoprotein abnormalities. However, the HPLC method can be useful and informative for analysis of abnormal lipoproteins.
KW - Dysbetalipoproteinemia
KW - HPLC
KW - LDL-cholesterol determination
KW - Reference method
UR - http://www.scopus.com/inward/record.url?scp=47749119129&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47749119129&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2008.05.008
DO - 10.1016/j.cca.2008.05.008
M3 - Article
VL - 395
SP - 62
EP - 67
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 1-2
ER -