Evaluation of an HPLC method for LDL-cholesterol determination in patients with various lipoprotein abnormalities in comparison with beta-quantification

Mitsuyo Okazaki, Shinichi Usui, Masakazu Nakamura, Shizuya Yamashita

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: The main purpose of this study was to evaluate an HPLC method for LDL-cholesterol determination in the presence of abnormal lipoproteins. Methods: We compared LDL-cholesterol levels obtained by HPLC (HPLC-LDL), Friedewald (F-LDL), and β-quantification (BQ-LDL) methods on 47 healthy volunteers and 50 outpatients with lipid disorders, including apolipoprotein E2/2 phenotype, cholesteryl ester transfer protein deficiency and lipoprotein lipase deficiency. Results: For the control group (n = 50), the HPLC-LDL and the F-LDL correlated highly with the BQ-LDL (r = 0.984 and 0.969, respectively), but the HPLC-LDL was lower than the BQ-LDL (mean bias: - 4.0%, P <0.001). For the subjects with lipoprotein abnormalities, significant biases were found in HPLC-LDL for the hypertriglyceridemia (+ 25%, n = 17, P <0.01), the hyper HDL (- 15.2%, n = 10, P <0.01) and the hyper lipoprotein(a) groups (- 13.4%, n = 12, P <0.001). The F-LDL was significantly higher than the BQ-LDL in the apolipoprotein E2/2 subjects (+ 92%, n = 8, P <0.001), but not significantly different in other subjects with triglycerides <4000 mg/l. Conclusions: There were several discrepancies in LDL-cholesterol levels determined by the HPLC and the BQ methods in samples with lipoprotein abnormalities. However, the HPLC method can be useful and informative for analysis of abnormal lipoproteins.

Original languageEnglish
Pages (from-to)62-67
Number of pages6
JournalClinica Chimica Acta
Volume395
Issue number1-2
DOIs
Publication statusPublished - Sep 2008

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LDL Cholesterol
Lipoproteins
High Pressure Liquid Chromatography
Apolipoprotein E2
Hyperlipoproteinemia Type I
Cholesterol Ester Transfer Proteins
oxidized low density lipoprotein
Lipoprotein(a)
Lipoprotein Lipase
Hypertriglyceridemia
Healthy Volunteers
Triglycerides
Outpatients
Phenotype
Lipids
Control Groups

Keywords

  • Dysbetalipoproteinemia
  • HPLC
  • LDL-cholesterol determination
  • Reference method

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Evaluation of an HPLC method for LDL-cholesterol determination in patients with various lipoprotein abnormalities in comparison with beta-quantification. / Okazaki, Mitsuyo; Usui, Shinichi; Nakamura, Masakazu; Yamashita, Shizuya.

In: Clinica Chimica Acta, Vol. 395, No. 1-2, 09.2008, p. 62-67.

Research output: Contribution to journalArticle

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N2 - Background: The main purpose of this study was to evaluate an HPLC method for LDL-cholesterol determination in the presence of abnormal lipoproteins. Methods: We compared LDL-cholesterol levels obtained by HPLC (HPLC-LDL), Friedewald (F-LDL), and β-quantification (BQ-LDL) methods on 47 healthy volunteers and 50 outpatients with lipid disorders, including apolipoprotein E2/2 phenotype, cholesteryl ester transfer protein deficiency and lipoprotein lipase deficiency. Results: For the control group (n = 50), the HPLC-LDL and the F-LDL correlated highly with the BQ-LDL (r = 0.984 and 0.969, respectively), but the HPLC-LDL was lower than the BQ-LDL (mean bias: - 4.0%, P <0.001). For the subjects with lipoprotein abnormalities, significant biases were found in HPLC-LDL for the hypertriglyceridemia (+ 25%, n = 17, P <0.01), the hyper HDL (- 15.2%, n = 10, P <0.01) and the hyper lipoprotein(a) groups (- 13.4%, n = 12, P <0.001). The F-LDL was significantly higher than the BQ-LDL in the apolipoprotein E2/2 subjects (+ 92%, n = 8, P <0.001), but not significantly different in other subjects with triglycerides <4000 mg/l. Conclusions: There were several discrepancies in LDL-cholesterol levels determined by the HPLC and the BQ methods in samples with lipoprotein abnormalities. However, the HPLC method can be useful and informative for analysis of abnormal lipoproteins.

AB - Background: The main purpose of this study was to evaluate an HPLC method for LDL-cholesterol determination in the presence of abnormal lipoproteins. Methods: We compared LDL-cholesterol levels obtained by HPLC (HPLC-LDL), Friedewald (F-LDL), and β-quantification (BQ-LDL) methods on 47 healthy volunteers and 50 outpatients with lipid disorders, including apolipoprotein E2/2 phenotype, cholesteryl ester transfer protein deficiency and lipoprotein lipase deficiency. Results: For the control group (n = 50), the HPLC-LDL and the F-LDL correlated highly with the BQ-LDL (r = 0.984 and 0.969, respectively), but the HPLC-LDL was lower than the BQ-LDL (mean bias: - 4.0%, P <0.001). For the subjects with lipoprotein abnormalities, significant biases were found in HPLC-LDL for the hypertriglyceridemia (+ 25%, n = 17, P <0.01), the hyper HDL (- 15.2%, n = 10, P <0.01) and the hyper lipoprotein(a) groups (- 13.4%, n = 12, P <0.001). The F-LDL was significantly higher than the BQ-LDL in the apolipoprotein E2/2 subjects (+ 92%, n = 8, P <0.001), but not significantly different in other subjects with triglycerides <4000 mg/l. Conclusions: There were several discrepancies in LDL-cholesterol levels determined by the HPLC and the BQ methods in samples with lipoprotein abnormalities. However, the HPLC method can be useful and informative for analysis of abnormal lipoproteins.

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