Abstract
The involvement of reactive oxygen species (ROS) and oxidative stress in pediatric diseases is an important concern, but oxidative stress status in healthy young subjects and appropriate methods for its measurement remain unclear. This study evaluated a comprehensive set of urinary biomarkers for oxidative stress in healthy children, adolescents and young adults. Results show that urinary excretion of acrolein-lysine, 8-hydroxy-2′-deoxyguanosine (8-OHdG), nitrite/nitrate and pentosidine were highest in the youngest subjects and decreased to constant levels by early adolescence. Urinary acrolein-lysine, 8-OHdG, nitrite/nitrate and pentosidine showed significant inverse correlations with age, but pyrraline did not change significantly with age. No significant differences in biomarkers were apparent between males and females. Younger subjects grow rapidly and sustain immune activation, and are probably exposed to high concentrations of ROS and nitric oxide. Consequently, they are more vulnerable to oxidation of lipids, proteins, DNA and carbohydrates. Normal reported values in this study are a basis for future studies of disease mechanisms involving oxidative stress and for future trials using antioxidant therapies for oxidative stress-related diseases in the pediatric field.
| Original language | English |
|---|---|
| Pages (from-to) | 1198-1205 |
| Number of pages | 8 |
| Journal | Free Radical Research |
| Volume | 40 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 2006 |
| Externally published | Yes |
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Keywords
- Biomarkers
- Oxidative stress
- Urine
- Young subjects
ASJC Scopus subject areas
- Biochemistry
Cite this
Evaluation of a urinary multi-parameter biomarker set for oxidative stress in children, adolescents and young adults. / Tamura, Satoshi; Tsukahara, Hirokazu; Ueno, Masaki; Maeda, Masayuki; Kawakami, Hisako; Sekine, Kyouichi; Mayumi, Mitsufumi.
In: Free Radical Research, Vol. 40, No. 11, 11.2006, p. 1198-1205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of a urinary multi-parameter biomarker set for oxidative stress in children, adolescents and young adults
AU - Tamura, Satoshi
AU - Tsukahara, Hirokazu
AU - Ueno, Masaki
AU - Maeda, Masayuki
AU - Kawakami, Hisako
AU - Sekine, Kyouichi
AU - Mayumi, Mitsufumi
PY - 2006/11
Y1 - 2006/11
N2 - The involvement of reactive oxygen species (ROS) and oxidative stress in pediatric diseases is an important concern, but oxidative stress status in healthy young subjects and appropriate methods for its measurement remain unclear. This study evaluated a comprehensive set of urinary biomarkers for oxidative stress in healthy children, adolescents and young adults. Results show that urinary excretion of acrolein-lysine, 8-hydroxy-2′-deoxyguanosine (8-OHdG), nitrite/nitrate and pentosidine were highest in the youngest subjects and decreased to constant levels by early adolescence. Urinary acrolein-lysine, 8-OHdG, nitrite/nitrate and pentosidine showed significant inverse correlations with age, but pyrraline did not change significantly with age. No significant differences in biomarkers were apparent between males and females. Younger subjects grow rapidly and sustain immune activation, and are probably exposed to high concentrations of ROS and nitric oxide. Consequently, they are more vulnerable to oxidation of lipids, proteins, DNA and carbohydrates. Normal reported values in this study are a basis for future studies of disease mechanisms involving oxidative stress and for future trials using antioxidant therapies for oxidative stress-related diseases in the pediatric field.
AB - The involvement of reactive oxygen species (ROS) and oxidative stress in pediatric diseases is an important concern, but oxidative stress status in healthy young subjects and appropriate methods for its measurement remain unclear. This study evaluated a comprehensive set of urinary biomarkers for oxidative stress in healthy children, adolescents and young adults. Results show that urinary excretion of acrolein-lysine, 8-hydroxy-2′-deoxyguanosine (8-OHdG), nitrite/nitrate and pentosidine were highest in the youngest subjects and decreased to constant levels by early adolescence. Urinary acrolein-lysine, 8-OHdG, nitrite/nitrate and pentosidine showed significant inverse correlations with age, but pyrraline did not change significantly with age. No significant differences in biomarkers were apparent between males and females. Younger subjects grow rapidly and sustain immune activation, and are probably exposed to high concentrations of ROS and nitric oxide. Consequently, they are more vulnerable to oxidation of lipids, proteins, DNA and carbohydrates. Normal reported values in this study are a basis for future studies of disease mechanisms involving oxidative stress and for future trials using antioxidant therapies for oxidative stress-related diseases in the pediatric field.
KW - Biomarkers
KW - Oxidative stress
KW - Urine
KW - Young subjects
UR - http://www.scopus.com/inward/record.url?scp=33749360328&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749360328&partnerID=8YFLogxK
U2 - 10.1080/10715760600895191
DO - 10.1080/10715760600895191
M3 - Article
C2 - 17050173
AN - SCOPUS:33749360328
VL - 40
SP - 1198
EP - 1205
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 11
ER -