Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method

Shinichi Usui, Tetsuya Mizuno, Mitsuyo Okazaki, Masakazu Nakamura, Ikunosuke Sakurabayashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives: To evaluate the gel permeation high-performance liquid chromatography (GP-HPLC) method for determination of triglyceride (TG) levels in low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Design and methods: The GP-HPLC and the ultracentrifugation(UC)/precipitation methods were used and compared. Results: There was no significant difference in measured levels of LDL-triglyceride between the two methods, but the HDL-triglyceride levels measured by the GP-HPLC technique were significantly higher than the UC/precipitation one (145 ± 47 mg/L and 121 ± 45 mg/L respectively, n = 38, p <0.0001). Conclusions: A GP-HPLC technique provides LDL-TG levels comparable to those obtained by the UC/precipitation method.

Original languageEnglish
Pages (from-to)114-117
Number of pages4
JournalClinical Biochemistry
Volume42
Issue number1-2
DOIs
Publication statusPublished - Jan 2009

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Ultracentrifugation
High performance liquid chromatography
Permeation
Lipoproteins
Triglycerides
Gels
High Pressure Liquid Chromatography
HDL Lipoproteins
Serum
LDL Lipoproteins
low density lipoprotein triglyceride

Keywords

  • Cholesterol
  • HPLC
  • Lipoprotein
  • Triglyceride

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method. / Usui, Shinichi; Mizuno, Tetsuya; Okazaki, Mitsuyo; Nakamura, Masakazu; Sakurabayashi, Ikunosuke.

In: Clinical Biochemistry, Vol. 42, No. 1-2, 01.2009, p. 114-117.

Research output: Contribution to journalArticle

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