Evaluation of a filament perforation model for mouse subarachnoid hemorrhage using 7.0 Tesla MRI

Carl Muroi, Yuto Kashiwagi, Takemi Rokugawa, Misato Tonomura, Atsushi Obata, Edin Nevzati, Akio Tsuboi, Kazuo Okuchi, Kenichi Mishima, Koji Abe, Masayuki Fujioka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The filament perforation model (FPM) in mice is becoming increasingly popular to elucidate the molecular pathogenesis of neuronal injury after subarachnoid hemorrhage (SAH). We evaluated brain MRI in a mouse FPM. A total of 28 male C57Bl/6J mice were used. Seventeen animals underwent SAH induction by FPM. In two animals, transient middle cerebral artery occlusion (MCAo) was induced. Nine mice served as controls. T1-weighted images (T1WI), T2-weighted images (T2WI), T2*-weighted images (T2*WI) and apparent diffusion coefficient maps were acquired at day 0 and at various time points following SAH (range: day 1-6 after SAH). Cerebral blood flow (CBF) analysis by 14C-iodoamphetamine (14C-IMP) autoradiography was conducted in nine animals. Hemorrhage could be best confirmed using T2*WI. The degree of hemorrhage varied. All animals evaluated for ≥2days were hydrocephalic, which was best seen on T2WI. T2-hyperintensity of the corpus callosum and external capsule, indicating white matter (WM) injury, was present after SAH. Ventricle and WM injury volumes were statistically significantly higher at day 3 compared to day 0. Territorial ischemia was detectable in MCAo but not in SAH. Markedly hypointense cortical veins were visible in the hyperacute and delayed phase after SAH on T2*WI. The 14C-IMP analysis indicated decreased CBF after SAH. MRI is feasible and useful in evaluating pathophysiological changes over time. T2*WI seems best for SAH detection and grading. The chronological change of hydrocephalus and WM injury could be analyzed. T2*WI illustrated specific signal changes of cortical veins, possibly caused by increased oxygen extraction fraction due to decreased CBF.

Original languageEnglish
JournalJournal of Clinical Neuroscience
DOIs
Publication statusAccepted/In press - Jun 4 2015
Externally publishedYes

Keywords

  • Filament perforation model
  • Magnetic resonance imaging
  • Mice
  • Subarachnoid hemorrhage

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Evaluation of a filament perforation model for mouse subarachnoid hemorrhage using 7.0 Tesla MRI'. Together they form a unique fingerprint.

  • Cite this

    Muroi, C., Kashiwagi, Y., Rokugawa, T., Tonomura, M., Obata, A., Nevzati, E., Tsuboi, A., Okuchi, K., Mishima, K., Abe, K., & Fujioka, M. (Accepted/In press). Evaluation of a filament perforation model for mouse subarachnoid hemorrhage using 7.0 Tesla MRI. Journal of Clinical Neuroscience. https://doi.org/10.1016/j.jocn.2015.10.045