TY - JOUR
T1 - Evaluation of a filament perforation model for mouse subarachnoid hemorrhage using 7.0 Tesla MRI
AU - Muroi, Carl
AU - Kashiwagi, Yuto
AU - Rokugawa, Takemi
AU - Tonomura, Misato
AU - Obata, Atsushi
AU - Nevzati, Edin
AU - Tsuboi, Akio
AU - Okuchi, Kazuo
AU - Mishima, Kenichi
AU - Abe, Kohji
AU - Fujioka, Masayuki
N1 - Funding Information:
C.M. was supported by a personal research grant from the Swiss Foundation for Grants in Biology and Medicine. The contribution of M.F. to this work was partly supported by the Japan Society for the Promotion of Science. The other authors declare that they have no financial or other conflicts of interest in relation to this research and its publication.
Publisher Copyright:
© 2016 Elsevier Ltd. All rights reserved.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - The filament perforation model (FPM) in mice is becoming increasingly popular to elucidate the molecular pathogenesis of neuronal injury after subarachnoid hemorrhage (SAH). We evaluated brain MRI in a mouse FPM. A total of 28 male C57Bl/6J mice were used. Seventeen animals underwent SAH induction by FPM. In two animals, transient middle cerebral artery occlusion (MCAo) was induced. Nine mice served as controls. T1-weighted images (T1WI), T2-weighted images (T2WI), T2 â - weighted images (T2∗WI) and apparent diffusion coefficient maps were acquired at day 0 and at various time points following SAH (range: day 1-6 after SAH). Cerebral blood flow (CBF) analysis by 14C-iodoamphetamine (14C-IMP) autoradiography was conducted in nine animals. Hemorrhage could be best confirmed using T2∗WI. The degree of hemorrhage varied. All animals evaluated for ≥2 days were hydrocephalic, which was best seen on T2WI. T2-hyperintensity of the corpus callosum and external capsule, indicating white matter (WM) injury, was present after SAH. Ventricle and WM injury volumes were statistically significantly higher at day 3 compared to day 0. Territorial ischemia was detectable in MCAo but not in SAH. Markedly hypointense cortical veins were visible in the hyperacute and delayed phase after SAH on T2∗WI. The 14C-IMP analysis indicated decreased CBF after SAH. MRI is feasible and useful in evaluating pathophysiological changes over time. T2∗WI seems best for SAH detection and grading. The chronological change of hydrocephalus and WM injury could be analyzed. T2∗WI illustrated specific signal changes of cortical veins, possibly caused by increased oxygen extraction fraction due to decreased CBF.
AB - The filament perforation model (FPM) in mice is becoming increasingly popular to elucidate the molecular pathogenesis of neuronal injury after subarachnoid hemorrhage (SAH). We evaluated brain MRI in a mouse FPM. A total of 28 male C57Bl/6J mice were used. Seventeen animals underwent SAH induction by FPM. In two animals, transient middle cerebral artery occlusion (MCAo) was induced. Nine mice served as controls. T1-weighted images (T1WI), T2-weighted images (T2WI), T2 â - weighted images (T2∗WI) and apparent diffusion coefficient maps were acquired at day 0 and at various time points following SAH (range: day 1-6 after SAH). Cerebral blood flow (CBF) analysis by 14C-iodoamphetamine (14C-IMP) autoradiography was conducted in nine animals. Hemorrhage could be best confirmed using T2∗WI. The degree of hemorrhage varied. All animals evaluated for ≥2 days were hydrocephalic, which was best seen on T2WI. T2-hyperintensity of the corpus callosum and external capsule, indicating white matter (WM) injury, was present after SAH. Ventricle and WM injury volumes were statistically significantly higher at day 3 compared to day 0. Territorial ischemia was detectable in MCAo but not in SAH. Markedly hypointense cortical veins were visible in the hyperacute and delayed phase after SAH on T2∗WI. The 14C-IMP analysis indicated decreased CBF after SAH. MRI is feasible and useful in evaluating pathophysiological changes over time. T2∗WI seems best for SAH detection and grading. The chronological change of hydrocephalus and WM injury could be analyzed. T2∗WI illustrated specific signal changes of cortical veins, possibly caused by increased oxygen extraction fraction due to decreased CBF.
KW - Filament perforation model
KW - Magnetic resonance imaging
KW - Mice
KW - Subarachnoid hemorrhage
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U2 - 10.1016/j.jocn.2015.10.045
DO - 10.1016/j.jocn.2015.10.045
M3 - Article
C2 - 27021225
AN - SCOPUS:84961773421
VL - 28
SP - 141
EP - 147
JO - Journal of Clinical Neuroscience
JF - Journal of Clinical Neuroscience
SN - 0967-5868
ER -