Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens

Shin Watanabe, Tsuyoshi Mizoguchi, Koh Aoki, Yasutaka Kubo, Hitoshi Mori, Shunsuke Imanishi, Yukiko Yamazaki, Daisuke Shibata, Hiroshi Ezura

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Sequencing a whole genome, cataloguing full-length cDNAs and ESTs, and construction of a comprehensive mutant population are essential steps in genome projects of individual model species. Tomato is one of the most important model crops to be undertaken for study over the next few decades, and the Solanaceae Genomics Project (SOL) has begun genome projects as international collaborations. The Solanum lycopersicum cultivar Micro-Tom shares several advantages with Arabidopsis, including its small size, short life cycle (approximately 90 days), and growth normally under artificial light. Here we demonstrate an initial attempt to generate a comprehensive mutant population in Micro-Tom. A total of 3,839 M2 families derived from ethylmethanesulfonate (EMS) mutagenesis were visually phenotyped, and putative mutants were classified into 15 primary and 48 secondary categories based on the SOL database, "The Genes That Make Tomatoes".

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalPlant Biotechnology
Volume24
Issue number1
Publication statusPublished - 2007

Fingerprint

Solanum lycopersicum
Lycopersicon esculentum
mutagenesis
Mutagenesis
Genome
mutants
genome
Cataloging
tomatoes
Solanaceae
Expressed Sequence Tags
crop models
Genomics
Life Cycle Stages
Arabidopsis
Population
life cycle (organisms)
Complementary DNA
Databases
genomics

Keywords

  • Ethylmethanesulfonate (EMS)
  • Mutant
  • Solanum lycopersicum cv. Micro-Tom

ASJC Scopus subject areas

  • Plant Science

Cite this

Watanabe, S., Mizoguchi, T., Aoki, K., Kubo, Y., Mori, H., Imanishi, S., ... Ezura, H. (2007). Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens. Plant Biotechnology, 24(1), 33-38.

Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens. / Watanabe, Shin; Mizoguchi, Tsuyoshi; Aoki, Koh; Kubo, Yasutaka; Mori, Hitoshi; Imanishi, Shunsuke; Yamazaki, Yukiko; Shibata, Daisuke; Ezura, Hiroshi.

In: Plant Biotechnology, Vol. 24, No. 1, 2007, p. 33-38.

Research output: Contribution to journalArticle

Watanabe, S, Mizoguchi, T, Aoki, K, Kubo, Y, Mori, H, Imanishi, S, Yamazaki, Y, Shibata, D & Ezura, H 2007, 'Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens', Plant Biotechnology, vol. 24, no. 1, pp. 33-38.
Watanabe, Shin ; Mizoguchi, Tsuyoshi ; Aoki, Koh ; Kubo, Yasutaka ; Mori, Hitoshi ; Imanishi, Shunsuke ; Yamazaki, Yukiko ; Shibata, Daisuke ; Ezura, Hiroshi. / Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens. In: Plant Biotechnology. 2007 ; Vol. 24, No. 1. pp. 33-38.
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