Abstract
Sequencing a whole genome, cataloguing full-length cDNAs and ESTs, and construction of a comprehensive mutant population are essential steps in genome projects of individual model species. Tomato is one of the most important model crops to be undertaken for study over the next few decades, and the Solanaceae Genomics Project (SOL) has begun genome projects as international collaborations. The Solanum lycopersicum cultivar Micro-Tom shares several advantages with Arabidopsis, including its small size, short life cycle (approximately 90 days), and growth normally under artificial light. Here we demonstrate an initial attempt to generate a comprehensive mutant population in Micro-Tom. A total of 3,839 M2 families derived from ethylmethanesulfonate (EMS) mutagenesis were visually phenotyped, and putative mutants were classified into 15 primary and 48 secondary categories based on the SOL database, "The Genes That Make Tomatoes".
| Original language | English |
|---|---|
| Pages (from-to) | 33-38 |
| Number of pages | 6 |
| Journal | Plant Biotechnology |
| Volume | 24 |
| Issue number | 1 |
| Publication status | Published - 2007 |
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Keywords
- Ethylmethanesulfonate (EMS)
- Mutant
- Solanum lycopersicum cv. Micro-Tom
ASJC Scopus subject areas
- Plant Science
Cite this
Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens. / Watanabe, Shin; Mizoguchi, Tsuyoshi; Aoki, Koh; Kubo, Yasutaka; Mori, Hitoshi; Imanishi, Shunsuke; Yamazaki, Yukiko; Shibata, Daisuke; Ezura, Hiroshi.
In: Plant Biotechnology, Vol. 24, No. 1, 2007, p. 33-38.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens
AU - Watanabe, Shin
AU - Mizoguchi, Tsuyoshi
AU - Aoki, Koh
AU - Kubo, Yasutaka
AU - Mori, Hitoshi
AU - Imanishi, Shunsuke
AU - Yamazaki, Yukiko
AU - Shibata, Daisuke
AU - Ezura, Hiroshi
PY - 2007
Y1 - 2007
N2 - Sequencing a whole genome, cataloguing full-length cDNAs and ESTs, and construction of a comprehensive mutant population are essential steps in genome projects of individual model species. Tomato is one of the most important model crops to be undertaken for study over the next few decades, and the Solanaceae Genomics Project (SOL) has begun genome projects as international collaborations. The Solanum lycopersicum cultivar Micro-Tom shares several advantages with Arabidopsis, including its small size, short life cycle (approximately 90 days), and growth normally under artificial light. Here we demonstrate an initial attempt to generate a comprehensive mutant population in Micro-Tom. A total of 3,839 M2 families derived from ethylmethanesulfonate (EMS) mutagenesis were visually phenotyped, and putative mutants were classified into 15 primary and 48 secondary categories based on the SOL database, "The Genes That Make Tomatoes".
AB - Sequencing a whole genome, cataloguing full-length cDNAs and ESTs, and construction of a comprehensive mutant population are essential steps in genome projects of individual model species. Tomato is one of the most important model crops to be undertaken for study over the next few decades, and the Solanaceae Genomics Project (SOL) has begun genome projects as international collaborations. The Solanum lycopersicum cultivar Micro-Tom shares several advantages with Arabidopsis, including its small size, short life cycle (approximately 90 days), and growth normally under artificial light. Here we demonstrate an initial attempt to generate a comprehensive mutant population in Micro-Tom. A total of 3,839 M2 families derived from ethylmethanesulfonate (EMS) mutagenesis were visually phenotyped, and putative mutants were classified into 15 primary and 48 secondary categories based on the SOL database, "The Genes That Make Tomatoes".
KW - Ethylmethanesulfonate (EMS)
KW - Mutant
KW - Solanum lycopersicum cv. Micro-Tom
UR - http://www.scopus.com/inward/record.url?scp=33947540242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947540242&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33947540242
VL - 24
SP - 33
EP - 38
JO - Plant Biotechnology
JF - Plant Biotechnology
SN - 1342-4580
IS - 1
ER -