Ethylene biosynthesis in detached young persimmon fruit is initiated in calyx and modulated by water loss from the fruit

Ryohei Nakano, Emi Ogura, Yasutaka Kubo, Akitsugu Inaba

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Persimmon (Diospyros kaki Thunb.) fruit are usually classified as climacteric fruit; however, unlike typical climacteric fruits, persimmon fruit exhibit a unique characteristic in that the younger the stage of fruit detached, the greater the level of ethylene produced. To investigate ethylene induction mechanisms in detached young persimmon fruit, we cloned three cDNAs encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (DK-ACS1, 2, and -3) and two encoding ACC oxidase (DK-ACO1 and -2) genes involved in ethylene biosynthesis, and we analyzed their expression in various fruit tissues. Ethylene production was induced within a few days of detachment in all fruit tissues tested, accompanied by temporally and spatially coordinated expression of all the DK-ACS and DK-ACO genes. In all tissues except the calyx, treatment with 1-methylcyclopropene, an inhibitor of ethylene action, suppressed ethylene production and ethylene biosynthesis-related gene expression. In the calyx, one ACC synthase gene (DK-ACS2) exhibited increased mRNA accumulation accompanied by a large quantity of ethylene production, and treatment of the fruit with 1-methylcyclopropene did not prevent either the accumulation of DK-ACS2 transcripts or ethylene induction. Furthermore, the alleviation of water loss from the fruit significantly delayed the onset of ethylene production and the expression of DK-ACS2 in the calyx. These results indicate that ethylene biosynthesis in detached young persimmon fruit is initially induced in calyx and is modulated by water loss through transcriptional activation of DK-ACS2. The ethylene produced in the calyx subsequently diffuses to other fruit tissues and acts as a secondary signal that stimulates autocatalytic ethylene biosynthesis in these tissues, leading to a burst of ethylene production.

Original languageEnglish
Pages (from-to)276-286
Number of pages11
JournalPlant Physiology
Volume131
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

Fingerprint

Diospyros
persimmons
calyx
ethylene production
Fruit
fruits
Water
water
ethylene
1-aminocyclopropane-1-carboxylic acid
1-methylcyclopropene
1-aminocyclopropane-1-carboxylate synthase
Diospyros kaki
ethylene inhibitors
genes
transcriptional activation
Genes
tissues

ASJC Scopus subject areas

  • Plant Science

Cite this

Ethylene biosynthesis in detached young persimmon fruit is initiated in calyx and modulated by water loss from the fruit. / Nakano, Ryohei; Ogura, Emi; Kubo, Yasutaka; Inaba, Akitsugu.

In: Plant Physiology, Vol. 131, No. 1, 01.01.2003, p. 276-286.

Research output: Contribution to journalArticle

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