Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium

Takahiro Shirozu, Hiroki Iwano, Takatoshi Ogiso, Toshiyuki Suzuki, Ahmed Z. Balboula, Hanako Bai, Manabu Kawahara, Koji Kimura, Hitomi Takahashi, Bai Rulan, Sung Woo Kim, Yojiro Yanagawa, Masashi Nagano, Kazuhiko Imakawa, Masashi Takahashi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Interferon tau (IFN-τ) is a ruminant-specific type I IFN secreted by a conceptus before its attachment to the uterus. IFN-τ induces the expression of IFN-stimulated genes (ISGs) via the type I IFN receptor (IFNAR), which is composed of IFNAR1 and IFNAR2 subunits in the endometrium. However, expression patterns of IFNARs during the estrous cycle have not been reported. We hypothesized that the response to a type I IFN changes along with IFNARs and the IFN-regulatory factors (IRFs) driving transcription of IFN signal-related genes and modulating a type I IFN signal during the estrous cycle. We investigated the estrous cycle stage-dependent type I IFN induction of ISGs and expression patterns of IFN signal-related genes in bovine endometrial tissues. Endometrial tissue pieces collected from bovine uteri at each estrous stage (early, mid, and late) were cultured with or without recombinant bovine IFN-α or concentrated pregnant uterine flushing (PUF) on day 18 after confirming the presence of a conceptus. IFN-α and PUF each significantly increased the expression of ISGs in endometrial tissues. The induction levels of the typical ISGs (MX1-a and ISG15) were significantly higher at the mid stage and correlated with high expression of IRFs at the mid stage. The immunostaining of IFNARs showed strong fluorescence intensities in luminal and glandular epithelia at the early and mid stages. Collectively, these results suggest that the endometrium exhibits estrous cycle stage-dependent responsiveness to type I IFN that may be associated with the expression of IFNARs and IRFs for pregnancy recognition.

Original languageEnglish
Pages (from-to)211-220
Number of pages10
JournalJournal of Reproduction and Development
Volume63
Issue number3
DOIs
Publication statusPublished - 2017

Fingerprint

gene induction
endometrium
interferons
estrous cycle
cattle
conceptus
genes
uterus
ruminants
epithelium
transcription factors
pregnancy
fluorescence
receptors
tissues

Keywords

  • Cow
  • Interferon-stimulated gene (ISG)
  • Type I Interferon (IFN)
  • Type I interferon receptor (IFNAR)
  • Uterus

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium. / Shirozu, Takahiro; Iwano, Hiroki; Ogiso, Takatoshi; Suzuki, Toshiyuki; Balboula, Ahmed Z.; Bai, Hanako; Kawahara, Manabu; Kimura, Koji; Takahashi, Hitomi; Rulan, Bai; Kim, Sung Woo; Yanagawa, Yojiro; Nagano, Masashi; Imakawa, Kazuhiko; Takahashi, Masashi.

In: Journal of Reproduction and Development, Vol. 63, No. 3, 2017, p. 211-220.

Research output: Contribution to journalArticle

Shirozu, T, Iwano, H, Ogiso, T, Suzuki, T, Balboula, AZ, Bai, H, Kawahara, M, Kimura, K, Takahashi, H, Rulan, B, Kim, SW, Yanagawa, Y, Nagano, M, Imakawa, K & Takahashi, M 2017, 'Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium', Journal of Reproduction and Development, vol. 63, no. 3, pp. 211-220. https://doi.org/10.1262/jrd.2016-176
Shirozu, Takahiro ; Iwano, Hiroki ; Ogiso, Takatoshi ; Suzuki, Toshiyuki ; Balboula, Ahmed Z. ; Bai, Hanako ; Kawahara, Manabu ; Kimura, Koji ; Takahashi, Hitomi ; Rulan, Bai ; Kim, Sung Woo ; Yanagawa, Yojiro ; Nagano, Masashi ; Imakawa, Kazuhiko ; Takahashi, Masashi. / Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium. In: Journal of Reproduction and Development. 2017 ; Vol. 63, No. 3. pp. 211-220.
@article{b001b231d81b4a42ac73a45ca1609e87,
title = "Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium",
abstract = "Interferon tau (IFN-τ) is a ruminant-specific type I IFN secreted by a conceptus before its attachment to the uterus. IFN-τ induces the expression of IFN-stimulated genes (ISGs) via the type I IFN receptor (IFNAR), which is composed of IFNAR1 and IFNAR2 subunits in the endometrium. However, expression patterns of IFNARs during the estrous cycle have not been reported. We hypothesized that the response to a type I IFN changes along with IFNARs and the IFN-regulatory factors (IRFs) driving transcription of IFN signal-related genes and modulating a type I IFN signal during the estrous cycle. We investigated the estrous cycle stage-dependent type I IFN induction of ISGs and expression patterns of IFN signal-related genes in bovine endometrial tissues. Endometrial tissue pieces collected from bovine uteri at each estrous stage (early, mid, and late) were cultured with or without recombinant bovine IFN-α or concentrated pregnant uterine flushing (PUF) on day 18 after confirming the presence of a conceptus. IFN-α and PUF each significantly increased the expression of ISGs in endometrial tissues. The induction levels of the typical ISGs (MX1-a and ISG15) were significantly higher at the mid stage and correlated with high expression of IRFs at the mid stage. The immunostaining of IFNARs showed strong fluorescence intensities in luminal and glandular epithelia at the early and mid stages. Collectively, these results suggest that the endometrium exhibits estrous cycle stage-dependent responsiveness to type I IFN that may be associated with the expression of IFNARs and IRFs for pregnancy recognition.",
keywords = "Cow, Interferon-stimulated gene (ISG), Type I Interferon (IFN), Type I interferon receptor (IFNAR), Uterus",
author = "Takahiro Shirozu and Hiroki Iwano and Takatoshi Ogiso and Toshiyuki Suzuki and Balboula, {Ahmed Z.} and Hanako Bai and Manabu Kawahara and Koji Kimura and Hitomi Takahashi and Bai Rulan and Kim, {Sung Woo} and Yojiro Yanagawa and Masashi Nagano and Kazuhiko Imakawa and Masashi Takahashi",
year = "2017",
doi = "10.1262/jrd.2016-176",
language = "English",
volume = "63",
pages = "211--220",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
publisher = "Japanese Society of Animal Reproduction (JSAR)",
number = "3",

}

TY - JOUR

T1 - Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium

AU - Shirozu, Takahiro

AU - Iwano, Hiroki

AU - Ogiso, Takatoshi

AU - Suzuki, Toshiyuki

AU - Balboula, Ahmed Z.

AU - Bai, Hanako

AU - Kawahara, Manabu

AU - Kimura, Koji

AU - Takahashi, Hitomi

AU - Rulan, Bai

AU - Kim, Sung Woo

AU - Yanagawa, Yojiro

AU - Nagano, Masashi

AU - Imakawa, Kazuhiko

AU - Takahashi, Masashi

PY - 2017

Y1 - 2017

N2 - Interferon tau (IFN-τ) is a ruminant-specific type I IFN secreted by a conceptus before its attachment to the uterus. IFN-τ induces the expression of IFN-stimulated genes (ISGs) via the type I IFN receptor (IFNAR), which is composed of IFNAR1 and IFNAR2 subunits in the endometrium. However, expression patterns of IFNARs during the estrous cycle have not been reported. We hypothesized that the response to a type I IFN changes along with IFNARs and the IFN-regulatory factors (IRFs) driving transcription of IFN signal-related genes and modulating a type I IFN signal during the estrous cycle. We investigated the estrous cycle stage-dependent type I IFN induction of ISGs and expression patterns of IFN signal-related genes in bovine endometrial tissues. Endometrial tissue pieces collected from bovine uteri at each estrous stage (early, mid, and late) were cultured with or without recombinant bovine IFN-α or concentrated pregnant uterine flushing (PUF) on day 18 after confirming the presence of a conceptus. IFN-α and PUF each significantly increased the expression of ISGs in endometrial tissues. The induction levels of the typical ISGs (MX1-a and ISG15) were significantly higher at the mid stage and correlated with high expression of IRFs at the mid stage. The immunostaining of IFNARs showed strong fluorescence intensities in luminal and glandular epithelia at the early and mid stages. Collectively, these results suggest that the endometrium exhibits estrous cycle stage-dependent responsiveness to type I IFN that may be associated with the expression of IFNARs and IRFs for pregnancy recognition.

AB - Interferon tau (IFN-τ) is a ruminant-specific type I IFN secreted by a conceptus before its attachment to the uterus. IFN-τ induces the expression of IFN-stimulated genes (ISGs) via the type I IFN receptor (IFNAR), which is composed of IFNAR1 and IFNAR2 subunits in the endometrium. However, expression patterns of IFNARs during the estrous cycle have not been reported. We hypothesized that the response to a type I IFN changes along with IFNARs and the IFN-regulatory factors (IRFs) driving transcription of IFN signal-related genes and modulating a type I IFN signal during the estrous cycle. We investigated the estrous cycle stage-dependent type I IFN induction of ISGs and expression patterns of IFN signal-related genes in bovine endometrial tissues. Endometrial tissue pieces collected from bovine uteri at each estrous stage (early, mid, and late) were cultured with or without recombinant bovine IFN-α or concentrated pregnant uterine flushing (PUF) on day 18 after confirming the presence of a conceptus. IFN-α and PUF each significantly increased the expression of ISGs in endometrial tissues. The induction levels of the typical ISGs (MX1-a and ISG15) were significantly higher at the mid stage and correlated with high expression of IRFs at the mid stage. The immunostaining of IFNARs showed strong fluorescence intensities in luminal and glandular epithelia at the early and mid stages. Collectively, these results suggest that the endometrium exhibits estrous cycle stage-dependent responsiveness to type I IFN that may be associated with the expression of IFNARs and IRFs for pregnancy recognition.

KW - Cow

KW - Interferon-stimulated gene (ISG)

KW - Type I Interferon (IFN)

KW - Type I interferon receptor (IFNAR)

KW - Uterus

UR - http://www.scopus.com/inward/record.url?scp=85021237330&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021237330&partnerID=8YFLogxK

U2 - 10.1262/jrd.2016-176

DO - 10.1262/jrd.2016-176

M3 - Article

C2 - 28239027

AN - SCOPUS:85021237330

VL - 63

SP - 211

EP - 220

JO - Journal of Reproduction and Development

JF - Journal of Reproduction and Development

SN - 0916-8818

IS - 3

ER -