TY - JOUR
T1 - Estrogen-related receptor α is essential for maintaining mitochondrial integrity in cisplatin-induced acute kidney injury
AU - Tsushida, Keigo
AU - Tanabe, Katsuyuki
AU - Masuda, Kana
AU - Tanimura, Satoshi
AU - Miyake, Hiromasa
AU - Arata, Yuka
AU - Sugiyama, Hitoshi
AU - Wada, Jun
N1 - Funding Information:
This study was supported by funds from JSPS KAKENHI Grant Number JP25860680 (2013–2014 to KT) and JP17K16088 (2017–2018 to KM).
Funding Information:
Jun Wada receives speaker honoraria from Astellas, Boehringer Ingelheim, Daiichi Sankyo, Novartis, and Tanabe Mitsubishi, and receives grant support from Astellas , Bayer , Baxter , Chugai , Daiichi-Sankyo , Kissei , Kyowa Hakko Kirin , MSD , Novartis , Novo Nordisk , Ono , Otsuka , Pfizer , Teijin , Torii , and Takeda .
Funding Information:
Jun Wada receives speaker honoraria from Astellas, Boehringer Ingelheim, Daiichi Sankyo, Novartis, and Tanabe Mitsubishi, and receives grant support from Astellas, Bayer, Baxter, Chugai, Daiichi-Sankyo, Kissei, Kyowa Hakko Kirin, MSD, Novartis, Novo Nordisk, Ono, Otsuka, Pfizer, Teijin, Torii, and Takeda.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/4/15
Y1 - 2018/4/15
N2 - Acute kidney injury (AKI) has been associated with not only higher in-hospital mortality but also the subsequent development of chronic kidney disease (CKD). Recent evidence has suggested the involvement of mitochondrial dysfunction and impaired dynamics in the pathogenesis of AKI. Estrogen-related receptor α (ERRα) is an orphan nuclear receptor that acts as a transcription factor to regulate the transcription of genes required for mitochondrial biogenesis and oxidative phosphorylation. In the present study, we examined the effects of ERRα deficiency on the progression of AKI induced by cisplatin. Male C57BL/6 J wild-type and ERRα−/- mice received a single intraperitoneal injection of 20 mg/kg cisplatin. Seventy-two hours after the injection, kidney function and morphology were evaluated. ERRα expression was observed in renal tubules, and cisplatin inhibited its translocation into nuclei. ERRα deficiency exacerbated cisplatin-induced renal dysfunction and tubular injury, as well as oxidative stress and apoptosis. ERRα−/- mice kidneys revealed lower mitochondrial DNA content and swollen mitochondria with reduced cristae. In addition, these mice had lower expression of the mitochondrial fusion protein mitofusin-2. The cisplatin-induced decrease in mitochondrial DNA and altered mitochondrial structure were more severe in ERRα−/- mice. In cultured mouse proximal tubular epithelial cells, the ERRα inverse agonist XCT-790 significantly inhibited mitofusin-2 expression and induced mitochondrial fragmentation. Taken together, our findings suggest the involvement of ERRα in the progression of cisplatin-induced AKI probably through impaired mitochondrial dynamics.
AB - Acute kidney injury (AKI) has been associated with not only higher in-hospital mortality but also the subsequent development of chronic kidney disease (CKD). Recent evidence has suggested the involvement of mitochondrial dysfunction and impaired dynamics in the pathogenesis of AKI. Estrogen-related receptor α (ERRα) is an orphan nuclear receptor that acts as a transcription factor to regulate the transcription of genes required for mitochondrial biogenesis and oxidative phosphorylation. In the present study, we examined the effects of ERRα deficiency on the progression of AKI induced by cisplatin. Male C57BL/6 J wild-type and ERRα−/- mice received a single intraperitoneal injection of 20 mg/kg cisplatin. Seventy-two hours after the injection, kidney function and morphology were evaluated. ERRα expression was observed in renal tubules, and cisplatin inhibited its translocation into nuclei. ERRα deficiency exacerbated cisplatin-induced renal dysfunction and tubular injury, as well as oxidative stress and apoptosis. ERRα−/- mice kidneys revealed lower mitochondrial DNA content and swollen mitochondria with reduced cristae. In addition, these mice had lower expression of the mitochondrial fusion protein mitofusin-2. The cisplatin-induced decrease in mitochondrial DNA and altered mitochondrial structure were more severe in ERRα−/- mice. In cultured mouse proximal tubular epithelial cells, the ERRα inverse agonist XCT-790 significantly inhibited mitofusin-2 expression and induced mitochondrial fragmentation. Taken together, our findings suggest the involvement of ERRα in the progression of cisplatin-induced AKI probably through impaired mitochondrial dynamics.
KW - Acute kidney injury
KW - Estrogen-related receptor α
KW - Mitochondria
KW - Mitofusin-2
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U2 - 10.1016/j.bbrc.2018.03.080
DO - 10.1016/j.bbrc.2018.03.080
M3 - Article
C2 - 29545177
AN - SCOPUS:85043994472
VL - 498
SP - 918
EP - 924
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -