Estrogen favors the differentiation of ovarian tissues in the ambisexual gonads of anemonefish Amphiprion clarkii

Saori Miura, Yasuhisa Kobayashi, Ramji Kumar Bhandari, Masaru Nakamura

Research output: Contribution to journalArticle

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Abstract

All undifferentiated gonads of anemonefish first differentiate into ovaries, and then testicular tissue appear among ovarian tissue, and finally form ambisexual gonads with both ovarian and testicular tissues. The role of estradiol-17beta (E2) in differentiation of ovarian cells is well conserved across phyla; however, its role in development of ambisexual gonads is poorly understood. Here we demonstrate that the E2 produced during the differentiation of ovarian cells does not allow testicular cells to differentiate in the prospective ambisexual gonad. We examined the immunolocalization of the steroidogenic enzyme cytochrome aromatase (P450arom), which is involved in E2 production. In the gonads, numbers of the P450arom-positive cells increased during ovarian differentiation. However, immunopositive cells with weak signal intensity were seen in the interstitial areas among oocytes and between oocytes and testicular tissue undergoing testicular differentiation. In contrast, P450arom-positive cells were not found in any testicular tissues of the ambisexual gonads. We also examined changes in E2 production in vitro in the gonads during testicular differentiation. E2 was high in the ovaries before the appearance all of testicular tissue, and decreased accompanying the differentiation of testicular tissue. These results suggest a balance of estrogen/androgen seems to be important during sex differentiation, and then a shift from estrogen to androgen production may induce testicular differentiation in the ovary. Further, exogenous E2 treatment suppressed naturally occurring differentiation of testicular cells forming exclusively ovarian tissues in the gonad in vivo, suggesting the increase of estrogen blocks the differentiation of testicular tissue and the formation of ambisexual gonad.

Original languageEnglish
Pages (from-to)560-568
Number of pages9
JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume319
Issue number10
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Amphiprion
Gonads
estrogens
gonads
Estrogens
Cell Differentiation
Ovary
cells
androgen
androgens
Androgens
Oocytes
oocytes
tissues
tissue
Sex Differentiation
Aromatase
sexual development
Cytochromes
cytochromes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Physiology
  • Molecular Biology

Cite this

Estrogen favors the differentiation of ovarian tissues in the ambisexual gonads of anemonefish Amphiprion clarkii. / Miura, Saori; Kobayashi, Yasuhisa; Bhandari, Ramji Kumar; Nakamura, Masaru.

In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Vol. 319, No. 10, 12.2013, p. 560-568.

Research output: Contribution to journalArticle

Miura, Saori ; Kobayashi, Yasuhisa ; Bhandari, Ramji Kumar ; Nakamura, Masaru. / Estrogen favors the differentiation of ovarian tissues in the ambisexual gonads of anemonefish Amphiprion clarkii. In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 2013 ; Vol. 319, No. 10. pp. 560-568.
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