Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro

Hidetaka Tasaki, Hisataka Iwata, Daichi Sato, Yasunori Monji, Takehito Kuwayama

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Antrum formation and estradiol (E2) secretion occur during early folliculogenesis. The objective was to determine the role of E2 in antrum formation of oocyte-granulosa cell complexes (OGCs) derived from porcine preantral follicles (PAFs). Supplementation of the culture medium with E2 (1 μg/mL) improved antrum formation of OGCs during 14 days of in vitro culture. Furthermore, adding 0.1 μg/mL androstenedione (a precursor of E2) to the medium also improved antrum formation. Concentration of E2 was higher in the medium of developmentally competent OGCs versus incompetent OGCs (8.5 vs. 3.5 ng/mL, P < 0.05). Fulvestrant (1 μg/mL), a competitive inhibitor of E2, completely inhibited antrum formation of OGCs that were cultured in medium containing either E2 (0.1 μg/mL) or androstenedione (0.1 μg/mL); however, increasing E2 to 1 μg/mL ameliorated the inhibitory effect. Conversely, in the case of early antral follicles, OGCs formed antrums without E2 supplementation. After E2 pretreatment, OGCs derived from PAFs formed antrums even when the OGCs were subsequently cultured in medium without E2. Furthermore, when OGCs derived from PAFs were cultured without E2 followed by an additional in vitro culture with E2, antrums were formed, albeit with the same period delay by the same pretreatment periods. In conclusion, E2 in the culture medium was indispensable for in vitro antrum formation of OGCs derived from PAFs; therefore, one of the roles of E2 is in the initiation of antrum formation.

Original languageEnglish
Pages (from-to)809-814
Number of pages6
JournalTheriogenology
Volume79
Issue number5
DOIs
Publication statusPublished - Mar 15 2013
Externally publishedYes

Fingerprint

Granulosa Cells
granulosa cells
Oocytes
estradiol
Estradiol
oocytes
Swine
swine
androstenedione
Androstenedione
in vitro culture
Culture Media
pretreatment
culture media
In Vitro Techniques
follicular development
secretion

Keywords

  • Antrum formation
  • Estradiol
  • Oocyte-granulosa cell complexes
  • Pig

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro. / Tasaki, Hidetaka; Iwata, Hisataka; Sato, Daichi; Monji, Yasunori; Kuwayama, Takehito.

In: Theriogenology, Vol. 79, No. 5, 15.03.2013, p. 809-814.

Research output: Contribution to journalArticle

Tasaki, Hidetaka ; Iwata, Hisataka ; Sato, Daichi ; Monji, Yasunori ; Kuwayama, Takehito. / Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro. In: Theriogenology. 2013 ; Vol. 79, No. 5. pp. 809-814.
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