Estradiol-17β is produced in bovine corpus luteum

K. Okuda, Y. Uenoyama, B. Berisha, I. G. Lange, H. Taniguchi, S. Kobayashi, S. I. Kobayashi, A. Miyamoto, D. Schams

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The aim of this study was to investigate the expression of cytochrome P450 aromatase (aromatase) mRNA, its activity, and estradiol-17β (estradiol) secretion in bovine corpus luteum (CL) during the estrous cycle. Expression of aromatase mRNA was examined in CL at the early, mid, late, and regressed luteal stages by using a reverse transcription-polymerase chain reaction. Aromatase mRNA was detected in all luteal stages examined, although aromatase expression was significantly lower during the early and regressed luteal phases compared to the mid and late luteal phases. Moreover, cultured midluteal cells clearly converted exogenous [3H]androstenedione into estradiol, and an aromatase inhibitor significantly inhibited this conversion. To characterize the local release of estradiol within the CL during the estrous cycle, an in vitro microdialysis system (MDS) of CL was conducted. Estradiol in MDS perfusate was confirmed by a reverse-phase high-performance liquid chromatography in combination with enzyme immunoassays. Basal release of estradiol from microdialyzed CL did not change during the estrous cycle. Additionally, when freshly prepared midluteal cells were exposed to estradiol (10-14 to 10-9 M), estradiol stimulated prostaglandin (PG) F secretion (P <0.05), although it did not affect progesterone and oxytocin secretion. The overall results indicate that estradiol is produced locally in bovine CL throughout the estrous cycle, and they suggest that estradiol plays a role in regulating PGF production in CL as an autocrine/paracrine factor.

Original languageEnglish
Pages (from-to)1634-1639
Number of pages6
JournalBiology of Reproduction
Volume65
Issue number6
Publication statusPublished - 2001

Fingerprint

Corpus Luteum
Estradiol
Aromatase
Estrous Cycle
Dinoprost
Luteal Phase
Microdialysis
Messenger RNA
Aromatase Inhibitors
Androstenedione
Oxytocin
Reverse-Phase Chromatography
Immunoenzyme Techniques
Cytochrome P-450 Enzyme System
Reverse Transcription
Progesterone
Cultured Cells
High Pressure Liquid Chromatography
Polymerase Chain Reaction

Keywords

  • Corpus luteum
  • Corpus luteum function
  • Estradiol
  • Ovary
  • Steroid hormones

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Okuda, K., Uenoyama, Y., Berisha, B., Lange, I. G., Taniguchi, H., Kobayashi, S., ... Schams, D. (2001). Estradiol-17β is produced in bovine corpus luteum. Biology of Reproduction, 65(6), 1634-1639.

Estradiol-17β is produced in bovine corpus luteum. / Okuda, K.; Uenoyama, Y.; Berisha, B.; Lange, I. G.; Taniguchi, H.; Kobayashi, S.; Kobayashi, S. I.; Miyamoto, A.; Schams, D.

In: Biology of Reproduction, Vol. 65, No. 6, 2001, p. 1634-1639.

Research output: Contribution to journalArticle

Okuda, K, Uenoyama, Y, Berisha, B, Lange, IG, Taniguchi, H, Kobayashi, S, Kobayashi, SI, Miyamoto, A & Schams, D 2001, 'Estradiol-17β is produced in bovine corpus luteum', Biology of Reproduction, vol. 65, no. 6, pp. 1634-1639.
Okuda K, Uenoyama Y, Berisha B, Lange IG, Taniguchi H, Kobayashi S et al. Estradiol-17β is produced in bovine corpus luteum. Biology of Reproduction. 2001;65(6):1634-1639.
Okuda, K. ; Uenoyama, Y. ; Berisha, B. ; Lange, I. G. ; Taniguchi, H. ; Kobayashi, S. ; Kobayashi, S. I. ; Miyamoto, A. ; Schams, D. / Estradiol-17β is produced in bovine corpus luteum. In: Biology of Reproduction. 2001 ; Vol. 65, No. 6. pp. 1634-1639.
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