TY - JOUR
T1 - Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia
AU - Tamatani, Takuya
AU - Kobayashi, Hiroyuki
AU - Tezuka, Katsunari
AU - Sakamoto, Shinji
AU - Suzuki, Kensuke
AU - Nakanishi, Tohru
AU - Takigawa, Masaharu
AU - Miyano, Takeshi
PY - 1998/10/29
Y1 - 1998/10/29
N2 - Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.
AB - Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.
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U2 - 10.1006/bbrc.1998.9543
DO - 10.1006/bbrc.1998.9543
M3 - Article
C2 - 9790981
AN - SCOPUS:0032578774
VL - 251
SP - 748
EP - 752
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -