Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

Takuya Tamatani; Hiroyuki Kobayashi; Katsunari Tezuka; Shinji Sakamoto; Kensuke Suzuki; Tohru Nakanishi; Masaharu Takigawa; Takeshi Miyano

doi:10.1006/bbrc.1998.9543

Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

Takuya Tamatani, Hiroyuki Kobayashi, Katsunari Tezuka, Shinji Sakamoto, Kensuke Suzuki, Tohru Nakanishi, Masaharu Takigawa, Takeshi Miyano

Dental School

Research output: Contribution to journal › Article › peer-review

118 Citations (Scopus)

Abstract

Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.

Original language	English
Pages (from-to)	748-752
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	251
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1998.9543
Publication status	Published - Oct 29 1998

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Tamatani, T., Kobayashi, H., Tezuka, K., Sakamoto, S., Suzuki, K., Nakanishi, T., Takigawa, M., & Miyano, T. (1998). Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia. Biochemical and Biophysical Research Communications, 251(3), 748-752. https://doi.org/10.1006/bbrc.1998.9543

Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia. / Tamatani, Takuya; Kobayashi, Hiroyuki; Tezuka, Katsunari; Sakamoto, Shinji; Suzuki, Kensuke; Nakanishi, Tohru; Takigawa, Masaharu; Miyano, Takeshi.

In: Biochemical and Biophysical Research Communications, Vol. 251, No. 3, 29.10.1998, p. 748-752.

Research output: Contribution to journal › Article › peer-review

Tamatani, T, Kobayashi, H, Tezuka, K, Sakamoto, S, Suzuki, K, Nakanishi, T, Takigawa, M & Miyano, T 1998, 'Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia', Biochemical and Biophysical Research Communications, vol. 251, no. 3, pp. 748-752. https://doi.org/10.1006/bbrc.1998.9543

Tamatani, Takuya ; Kobayashi, Hiroyuki ; Tezuka, Katsunari ; Sakamoto, Shinji ; Suzuki, Kensuke ; Nakanishi, Tohru ; Takigawa, Masaharu ; Miyano, Takeshi. / Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 251, No. 3. pp. 748-752.

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