Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

Takuya Tamatani; Hiroyuki Kobayashi; Katsunari Tezuka; Shinji Sakamoto; Kensuke Suzuki; Tohru Nakanishi; Masaharu Takigawa; Takeshi Miyano

doi:10.1006/bbrc.1998.9543

Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

Takuya Tamatani, Hiroyuki Kobayashi, Katsunari Tezuka, Shinji Sakamoto, Kensuke Suzuki, Tohru Nakanishi, Masaharu Takigawa, Takeshi Miyano

Dental School

Research output: Contribution to journal › Article › peer-review

120 Citations (Scopus)

Abstract

Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.

Original language	English
Pages (from-to)	748-752
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	251
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1998.9543
Publication status	Published - Oct 29 1998

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1998.9543

Cite this

Tamatani, T., Kobayashi, H., Tezuka, K., Sakamoto, S., Suzuki, K., Nakanishi, T., Takigawa, M., & Miyano, T. (1998). Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia. Biochemical and Biophysical Research Communications, 251(3), 748-752. https://doi.org/10.1006/bbrc.1998.9543

Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia. / Tamatani, Takuya; Kobayashi, Hiroyuki; Tezuka, Katsunari et al.

In: Biochemical and Biophysical Research Communications, Vol. 251, No. 3, 29.10.1998, p. 748-752.

Research output: Contribution to journal › Article › peer-review

Tamatani, T, Kobayashi, H, Tezuka, K, Sakamoto, S, Suzuki, K, Nakanishi, T, Takigawa, M & Miyano, T 1998, 'Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia', Biochemical and Biophysical Research Communications, vol. 251, no. 3, pp. 748-752. https://doi.org/10.1006/bbrc.1998.9543

@article{3ca5baa52dfb49b1aff98714aa0a205d,

title = "Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia",

abstract = "Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.",

author = "Takuya Tamatani and Hiroyuki Kobayashi and Katsunari Tezuka and Shinji Sakamoto and Kensuke Suzuki and Tohru Nakanishi and Masaharu Takigawa and Takeshi Miyano",

year = "1998",

month = oct,

day = "29",

doi = "10.1006/bbrc.1998.9543",

language = "English",

volume = "251",

pages = "748--752",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

AU - Tamatani, Takuya

AU - Kobayashi, Hiroyuki

AU - Tezuka, Katsunari

AU - Sakamoto, Shinji

AU - Suzuki, Kensuke

AU - Nakanishi, Tohru

AU - Takigawa, Masaharu

AU - Miyano, Takeshi

PY - 1998/10/29

Y1 - 1998/10/29

N2 - Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.

AB - Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.

UR - http://www.scopus.com/inward/record.url?scp=0032578774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032578774&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1998.9543

DO - 10.1006/bbrc.1998.9543

M3 - Article

C2 - 9790981

AN - SCOPUS:0032578774

VL - 251

SP - 748

EP - 752

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -

Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this