Abstract
Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.
| Original language | English |
|---|---|
| Pages (from-to) | 748-752 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 251 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Oct 29 1998 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Fingerprint Dive into the research topics of 'Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia'. Together they form a unique fingerprint.
Cite this
Tamatani, T., Kobayashi, H., Tezuka, K., Sakamoto, S., Suzuki, K., Nakanishi, T., Takigawa, M., & Miyano, T. (1998). Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia. Biochemical and Biophysical Research Communications, 251(3), 748-752. https://doi.org/10.1006/bbrc.1998.9543