Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

Takuya Tamatani, Hiroyuki Kobayashi, Katsunari Tezuka, Shinji Sakamoto, Kensuke Suzuki, Tohru Nakanishi, Masaharu Takigawa, Takeshi Miyano

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-β but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.

Original languageEnglish
Pages (from-to)748-752
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume251
Issue number3
DOIs
Publication statusPublished - Oct 29 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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