Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

Hirotsugu Takano, Shunsuke Nakazawa, Yasushi Okuno, Naritoshi Shirata, Sohken Tsuchiya, Takayuki Kainoh, Seigoh Takamatsu, Kazuyuki Furuta, Yoshitaka Taketomi, Yuko Naito, Hiromu Takematsu, Yasunori Kozutsumi, Gozoh Tsujimoto, Makoto Murakami, Ichiro Kudo, Atsushi Ichikawa, Kazuhisa Nakayama, Yukihiko Sugimoto, Satoshi Tanaka

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27 Citations (Scopus)

Abstract

To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

Original languageEnglish
Pages (from-to)1444-1450
Number of pages7
JournalFEBS Letters
Volume582
Issue number10
DOIs
Publication statusPublished - Apr 30 2008
Externally publishedYes

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Keywords

  • Differentiation
  • G
  • Gene expression
  • Mast cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Takano, H., Nakazawa, S., Okuno, Y., Shirata, N., Tsuchiya, S., Kainoh, T., Takamatsu, S., Furuta, K., Taketomi, Y., Naito, Y., Takematsu, H., Kozutsumi, Y., Tsujimoto, G., Murakami, M., Kudo, I., Ichikawa, A., Nakayama, K., Sugimoto, Y., & Tanaka, S. (2008). Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells. FEBS Letters, 582(10), 1444-1450. https://doi.org/10.1016/j.febslet.2008.03.033