Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro

Hidetsugu Tsujigiwa, Naoki Katase, Mathieu Lefeuvre, Eiki Yamachika, Ryo Tamamura, Satoshi Ito, Yuichiro Takebe, Hiroyuki Matsuda, Hitoshi Nagatsuka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tooth tissue engineering offers very attractive perspectives for elaboration of regenerative treatments, which enables to cure tooth loss and restore quality of life of the patients. To elaborate such treatment, isolation and culture of dental pulp cell must be achieved as a key element. In this article, we report the establishment of a stable cell line from GFP transgenic rat dental pulp, named TGC (Tooth Matrix-forming, GFP Rat-derived Cell). TGCs have exhibited odontoblastic feature both in vitro and in vivo. In vitro, TGC exposed to osteogenic medium demonstrated collagen fiber synthesis with matrix vesicle and mineralization and formed a sheet-like substrate on the cell culture dish. Increased ALP activity and elevated transcription level of various genes involved in calcification and dentin formation were also observed. In vivo, transplanted TGC in SCID mice with β-TCP particles formed dentin-like and pulp-like structure with lining odontoblast. Notably, even after up to 80 passages, TGCs retain their morphological features and differentiation ability. To our knowledge, this is the first report of a dental pulp-derived cell with such stable odontoblastic characteristics. TGC could be a very useful model for further study on dental pulp cell.

Original languageEnglish
Pages (from-to)799-806
Number of pages8
JournalJournal of Oral Pathology and Medicine
Volume42
Issue number10
DOIs
Publication statusPublished - Nov 2013

    Fingerprint

Keywords

  • Dental pulp
  • Matrix formation
  • Osteoblast
  • Stem cell-like cell

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics
  • Medicine(all)

Cite this