Establishment of ndart1-tagged lines of koshihikari, an elite variety of rice in japan

Hideki Nishimura, Eiko Himi, Kazuhide Rikiishi, Kazuo Tsugane, Masahiko Maekawa

Research output: Contribution to journalArticle

Abstract

To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.

Original languageEnglish
Pages (from-to)696-701
Number of pages6
JournalBreeding Science
Volume69
Issue number4
DOIs
Publication statusPublished - 2019

Keywords

  • DNA transposon
  • Insertional mutagenesis
  • Koshihikari
  • NDart1-tagged lines
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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