Establishment of monoclonal antibodies against human acidic fibroblast growth factor

Yuzo Ichimori, Yumiko Kinoshita, Tatsuya Watanabe, Masaharu Seno, Koichi Kondo

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9 Citations (Scopus)

Abstract

Four kinds of hybridomas secreting monoclonal antibodies (MAbs) against human acidic fibroblast growth factor (haFGF) were established using recombinant haFGF as an immunogen. The recognition sites of four MAbs designated AF1-52, 81, 114 and 1C10 for the haFGF molecule were examined by binding studies with synthetic polypeptides and with amino-terminal truncated forms of haFGF. These experiments suggested that AF1-52, 114, and 1C10 MAbs recognize epitopes within the 1-5, 44-132 and 6-43 amino acid sequences, respectively. However, the epitope recognized by the AF1-81 MAb could not be determined. The sandwich EIA method constructed with these MAbs was sensitive to 1.5 pg/well of haFGF and had no cross-reactivity with human basic FGF, bovine aFGF or the hst-1 gene product.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume175
Issue number1
DOIs
Publication statusPublished - Feb 28 1991

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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