Establishment of a tightly regulated human cell line for the development of hepatocyte transplantation.

N. Kobayashi, H. Noguchi, T. Watanabe, T. Matsumura, T. Totsugawa, Toshiyoshi Fujiwara, K. Westerman, P. Leboulch, I. J. Fox, N. Tanaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Hepatocyte transplantation (HTX) could be an attractive treatment for patients with liver failure and liver-based metabolic disease. Human primary hepatocytes are ideal in this modality, but the shortage of human livers available for hepatocyte isolation severely limits the use of this form of therapy. A tightly regulated human hepatocyte cell line that grows economically in culture and exhibits differentiated liver functions would be an attractive alternative to the primary human hepatocytes. To test the feasibility, human hepatocytes were immortalized by a retroviral vector expressing simian virus 40 large T antigen and herpes simplex virus-thymidine kinase. A highly differentiated immortal hepatocyte line NKNT-3 was established. NKNT-3 cells grew in chemically defined serum-free medium, retained highly differentiated liver functions, and were sensitivity to ganciclovir as a prodrug. Essentially unlimited availability of NKNT-3 cells may be clinically useful for HTX and bioartificial liver.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalHuman cell : official journal of Human Cell Research Society
Volume13
Issue number1
Publication statusPublished - Mar 2000

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Hepatocytes
Transplantation
Cell Line
Liver
Artificial Liver
Ganciclovir
Simian virus 40
Thymidine Kinase
Viral Tumor Antigens
Metabolic Diseases
Serum-Free Culture Media
Liver Failure
Prodrugs
Simplexvirus
Therapeutics

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

Cite this

Establishment of a tightly regulated human cell line for the development of hepatocyte transplantation. / Kobayashi, N.; Noguchi, H.; Watanabe, T.; Matsumura, T.; Totsugawa, T.; Fujiwara, Toshiyoshi; Westerman, K.; Leboulch, P.; Fox, I. J.; Tanaka, N.

In: Human cell : official journal of Human Cell Research Society, Vol. 13, No. 1, 03.2000, p. 7-13.

Research output: Contribution to journalArticle

Kobayashi, N, Noguchi, H, Watanabe, T, Matsumura, T, Totsugawa, T, Fujiwara, T, Westerman, K, Leboulch, P, Fox, IJ & Tanaka, N 2000, 'Establishment of a tightly regulated human cell line for the development of hepatocyte transplantation.', Human cell : official journal of Human Cell Research Society, vol. 13, no. 1, pp. 7-13.
Kobayashi, N. ; Noguchi, H. ; Watanabe, T. ; Matsumura, T. ; Totsugawa, T. ; Fujiwara, Toshiyoshi ; Westerman, K. ; Leboulch, P. ; Fox, I. J. ; Tanaka, N. / Establishment of a tightly regulated human cell line for the development of hepatocyte transplantation. In: Human cell : official journal of Human Cell Research Society. 2000 ; Vol. 13, No. 1. pp. 7-13.
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