Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation

Mika Kato Kaneko, Wei Tian, Shingo Takano, Hiroyuki Suzuki, Yoshihiko Sawa, Yasukazu Hozumi, Kaoru Goto, Kentaro Yamazaki, Chifumi Kitanaka, Yukinari Kato

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Isocitrate dehydrogenase 1 (IDH1) mutations, which are early and frequent genetic alterations in gliomas, are specific to a single codon in the conserved and functionally important Arginine 132 (R132) in IDH1. We earlier established a monoclonal antibody (mAb), IMab-1, which is specific for R132H-containing IDH1 (IDH1-R132H), the most frequent IDH1 mutation in gliomas. To establish IDH1-R132S-specific mAb, we immunized mice with R132S-containing IDH1 (IDH1-R132S) peptide. After cell fusion using Sendai virus envelope, IDH1-R132S-specific mAbs were screened in ELISA. One mAb, SMab-1, reacted with the IDH1-R132S peptide, but not with other IDH1 mutants. Western-blot analysis showed that SMab-1 reacted only with the IDH1-R132S protein, not with IDH1-WT protein or IDH1 mutants, indicating that SMab-1 is IDH1-R132S-specific. Furthermore, SMab-1 specifically stained the IDH1-R132S-expressing glioblastoma cells in immunocytochemistry and immunohistochemistry, but did not react with IDH1-WT or IDH1-R132H-containing glioblastoma cells. We newly established an anti-IDH1-R132S-specific mAb SMab-1 for use in diagnosis of mutation-bearing gliomas.

Original languageEnglish
Pages (from-to)608-613
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume406
Issue number4
DOIs
Publication statusPublished - Mar 25 2011
Externally publishedYes

Keywords

  • Glioblastoma
  • IDH1
  • Monoclonal antibody
  • Mutation
  • R132S

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kaneko, M. K., Tian, W., Takano, S., Suzuki, H., Sawa, Y., Hozumi, Y., Goto, K., Yamazaki, K., Kitanaka, C., & Kato, Y. (2011). Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation. Biochemical and Biophysical Research Communications, 406(4), 608-613. https://doi.org/10.1016/j.bbrc.2011.02.102