Establishment of a novel collagenase perfusion method to isolate rat pancreatic stellate cells and investigation of their gene expression of TGF-β1, type I collagen, and CTGF in primary culture or freshly isolated cells

Toshiyuki Shinji, Kozo Ujike, Koji Ochi, Nobuchika Kusano, Tetsuya Kikui, Naoki Matsumura, Yasuyuki Emori, Toshinobu Seno, Norio Koide

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In studies of the pathogenesis of pancreatic fibrosis, pancreatic stellate cells (PSCs) have recently gained attention. In the present study, we established a new collagenase perfusion method through thoracic aorta cannulation to isolate PSCs, and we studied gene expression of TGF-β1, type I collagen, and connective tissue growth factor using primary cultured PSCs. Our method facilitated PSC isolation, and by our new method, 4.3 ± 1.2 × 106 PSCs were obtained from a rat. In comparing the expression of these genes with that of hepatic stellate cells (HSCs), we observed a similar pattern, although PSCs expressed type I collagen gene earlier than did HSCs. These results suggest that PSCs may play an important role in fibrosis of the pancreas, as HSCs do in liver fibrosis; in addition, PSCs may exist in a preactivated state or may be more easily activated than are HSCs. We also isolated the PSCs from a WBN/Kob rat, the spontaneous pancreatitis rat, and compared the gene expression with that from a normal rat.

Original languageEnglish
Pages (from-to)211-218
Number of pages8
JournalActa medica Okayama
Volume56
Issue number4
Publication statusPublished - Dec 1 2002

Keywords

  • Collagenase perfusion
  • Connective tissue growth factor
  • Pancreatic stellate cell
  • Transforming growth factor beta
  • WBN/Kob rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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