Establishment of a new diagnostic method for hydropic villi by using TSSC3 antibody

Norio Wake, Tomoka Takao, Kazuo Asanoma, Hidenori Kato

Research output: Contribution to journalArticlepeer-review

Abstract

A total of 297 samples of hydropic villi were classified according to DNA polymorphisms as androgenetic moles, dispermic triploids, or biparental diploids. A subset of 267 appropriate samples was included in the study. Most of the macroscopically diagnosed complete mole cases were genetically androgenetic in origin. The partial mole cases consisted of 30 androgenetic moles and 12 dispermic triploids. For the 59 cases macroscopically categorized as hydropic abortion, the genetic analysis revealed 38 androgenetic moles, seven dispermic triploids and 14 biparental diploids. These results showed that a new diagnostic method was required for the management of patients with hydropic villi. We identified the TSSC imprint gene of which expression was shown in normal and partial mole villi but was silenced in complete mole villi. Immunohistochemistry using the TSSC3 antibody demonstrated its efficacy as the differential diagnostic method. TSSC3 play an important role in the differentiation from trophoblast stem cells to progenitors and/or labyrinth trophoblast through the TSSC3/PI3K/Akt/Mash2 signaling pathway.

Original languageEnglish
Pages (from-to)1230-1235
Number of pages6
JournalJournal of Obstetrics and Gynaecology Research
Volume39
Issue number7
DOIs
Publication statusPublished - Jul 2013
Externally publishedYes

Keywords

  • Androgenesis
  • Genome research
  • Hydropic villi
  • Immunohistochemistry
  • TSSC3

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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