Establishment of a monoclonal antibody against cd81 that decreases the proliferation of rat glioma cells

Erika Fujimoto, Hiroki Mori, Motoharu Takehara, Miyuki Tanaka, Toshitaka Oohashi, Yoshifumi Ninomiya, Tohru Nakanishi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

CD81 belongs to a family of cell-surface proteins (tetraspanins) known to be up-regulated in rheumatoid arthritis (RA) synoviocytes. Recently, we showed that small interfering RNA targeting CD81 (CD81 siRNA) has a therapeutic effect on RA. We also showed that CD81 siRNA decreased the expression of TNF- in SW982 cells, suggesting that TNF- stimulates the expression of synoviolin via CD81. Here, we show that a monoclonal antibody (MAb) against CD81, which was raised by immunization with recombinant CD81, decreased the proliferation of rat C6 glioma cells, showing that this antibody is functionally active and probably inhibits cell proliferation through the inhibition of CD81 function on the cell surface.

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalJournal of Hard Tissue Biology
Volume23
Issue number1
Publication statusPublished - 2014

Fingerprint

Monoclonal antibodies
Glioma
Small Interfering RNA
Rats
Monoclonal Antibodies
Immunization
Rheumatoid Arthritis
Cell proliferation
Tetraspanins
RNA
Antibodies
Therapeutic Uses
Proteins
Membrane Proteins
Cell Proliferation

Keywords

  • C6 glioma
  • Cd81
  • Monoclonal antibody
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Fujimoto, E., Mori, H., Takehara, M., Tanaka, M., Oohashi, T., Ninomiya, Y., & Nakanishi, T. (2014). Establishment of a monoclonal antibody against cd81 that decreases the proliferation of rat glioma cells. Journal of Hard Tissue Biology, 23(1), 131-134.

Establishment of a monoclonal antibody against cd81 that decreases the proliferation of rat glioma cells. / Fujimoto, Erika; Mori, Hiroki; Takehara, Motoharu; Tanaka, Miyuki; Oohashi, Toshitaka; Ninomiya, Yoshifumi; Nakanishi, Tohru.

In: Journal of Hard Tissue Biology, Vol. 23, No. 1, 2014, p. 131-134.

Research output: Contribution to journalArticle

Fujimoto, E, Mori, H, Takehara, M, Tanaka, M, Oohashi, T, Ninomiya, Y & Nakanishi, T 2014, 'Establishment of a monoclonal antibody against cd81 that decreases the proliferation of rat glioma cells', Journal of Hard Tissue Biology, vol. 23, no. 1, pp. 131-134.
Fujimoto, Erika ; Mori, Hiroki ; Takehara, Motoharu ; Tanaka, Miyuki ; Oohashi, Toshitaka ; Ninomiya, Yoshifumi ; Nakanishi, Tohru. / Establishment of a monoclonal antibody against cd81 that decreases the proliferation of rat glioma cells. In: Journal of Hard Tissue Biology. 2014 ; Vol. 23, No. 1. pp. 131-134.
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