Establishment of a Langerhans cell histiocytosis lesion cell line with dermal dendritic cell characteristics

Ichiro Murakami, Jean Gogusev, Francis Jaubert, Michiko Matsushita, Kazuhiko Hayashi, Ikuo Miura, Takehiro Tanaka, Takashi Oka, Tadashi Yoshino

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A cell line named PRU-1, derived from a Langerhans cell (LC) histiocytosis (LCH) skull lesion of a 7-year-old boy, was established and characterized. PRU-1 is an adherent spindle-shaped cell line that shows no Birbeck granules on electron microscopy. Flow cytometric analysis of cells collected from the early seventh passage showed no LC phenotypes of CD1a and S100 protein. Immunostaining of PRU-1 cells also revealed no expression of LC markers but showed expression of CD11c, CD54 (ICAM-1) and CD68, which was also observed in some peripherally located cells of the original LCH lesion. The PRU-1 cells stained positive for factor XIIIa and negative for CD34, suggesting a dermal dendritic cell phenotype. Cytogenetic analyses revealed abnormalities such as 39,XY,-2,-4,-8,-12,-12,-14,add(18)(q21),20,+mar and 44,XY,-11,-14,add(18)(q21). TCRγ rearrangement in the PRU-1 cells was not amplified by PCR. Tumorigenicity was not proven by xenografting into SCID mice. A conditioned medium from PRU-1 culture induced the proliferation of peripheral blood lymphocytes as well as the activation of monocytes from a healthy donor into CD1a-positive LC-like cells. Because the phenotypic characteristics of PRU-1 differed from those of CD1a-positive abnormal LC-like cells (LCH cells), it was likely that the PRU-1 cells were derived from peripherally located cells of the LCH lesion rather than LCH cells. LCH has been regarded as a type of granulomatous neoplasm with several intermingled inflammatory cells and influenced by stimuli such as Merkel cell polyomavirus (MCPyV) infection or cigarette smoking. However, in the PRU-1 cells, MCPyV-DNA was not detected by PCR. Stromal cell-like PRU-1 cells are likely to produce some growth or differentiation factors, which may play important roles in LCH lesion formation, cell maintenance and LC-like cell induction.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalOncology Reports
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Langerhans Cell Histiocytosis
Langerhans Cells
Cell Line
Histiocytosis
Merkel cell polyomavirus
Polyomavirus Infections
Factor XIIIa
Growth Differentiation Factors
Phenotype
Mars
Polymerase Chain Reaction
Heterologous Transplantation
SCID Mice
S100 Proteins
Cytogenetic Analysis
Intercellular Adhesion Molecule-1
Stromal Cells
Conditioned Culture Medium

Keywords

  • Cell line
  • Dermal dendritic cell
  • Langerhans cell histiocytosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Establishment of a Langerhans cell histiocytosis lesion cell line with dermal dendritic cell characteristics. / Murakami, Ichiro; Gogusev, Jean; Jaubert, Francis; Matsushita, Michiko; Hayashi, Kazuhiko; Miura, Ikuo; Tanaka, Takehiro; Oka, Takashi; Yoshino, Tadashi.

In: Oncology Reports, Vol. 33, No. 1, 01.01.2015, p. 171-178.

Research output: Contribution to journalArticle

Murakami, Ichiro ; Gogusev, Jean ; Jaubert, Francis ; Matsushita, Michiko ; Hayashi, Kazuhiko ; Miura, Ikuo ; Tanaka, Takehiro ; Oka, Takashi ; Yoshino, Tadashi. / Establishment of a Langerhans cell histiocytosis lesion cell line with dermal dendritic cell characteristics. In: Oncology Reports. 2015 ; Vol. 33, No. 1. pp. 171-178.
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