Establishment of a highly differentiated immortalized human hepatocyte cell line with tight regulation

N. Kobayashi, H. Noguchi, T. Fujiwara, N. Tanaka

Research output: Contribution to journalConference article

Abstract

To determine an ideal cell line for bioartificial liver (BAL) therapy, primary human hepatocytes were immortalized with a plasmid SV3neo containing the genes of simian virus 40 large T antigen and neomycin phosphototransferase by a method of lipofection. As a safeguard in clinical application, HSV-tk gene was retrovirally introduced into the immortal cells. The gene expression of liver-specific functions and in vitro sensitivity to ganciclovir (GCV) were examined. To investigate the in-vivo potential of cells, intrasplenic transplantation (Isp-Tx) was performed in rats that underwent 90% hepatectomy. The results indicate that the cells can offer the advantages of uniformity, sterility, unlimited availability, and freedom from infectious pathogens and may be useful for BAL.

Original languageEnglish
Number of pages1
JournalASAIO Journal
Volume46
Issue number2
DOIs
Publication statusPublished - Jan 1 2000
Event46th Annual Conference and Exposition of ASAIO - New York, NY, USA
Duration: Jun 28 2000Jul 1 2000

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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