Establishment of a highly differentiated immortalized human hepatocyte cell line with tight regulation

N. Kobayashi, H. Noguchi, Toshiyoshi Fujiwara, N. Tanaka

Research output: Contribution to journalArticle

Abstract

To determine an ideal cell line for bioartificial liver (BAL) therapy, primary human hepatocytes were immortalized with a plasmid SV3neo containing the genes of simian virus 40 large T antigen and neomycin phosphototransferase by a method of lipofection. As a safeguard in clinical application, HSV-tk gene was retrovirally introduced into the immortal cells. The gene expression of liver-specific functions and in vitro sensitivity to ganciclovir (GCV) were examined. To investigate the in-vivo potential of cells, intrasplenic transplantation (Isp-Tx) was performed in rats that underwent 90% hepatectomy. The results indicate that the cells can offer the advantages of uniformity, sterility, unlimited availability, and freedom from infectious pathogens and may be useful for BAL.

Original languageEnglish
Pages (from-to)229
Number of pages1
JournalASAIO Journal
Volume46
Issue number2
Publication statusPublished - Mar 2000

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Artificial Liver
Liver
Hepatocytes
Cells
Cell Line
Ganciclovir
Simian virus 40
Neomycin
Viral Tumor Antigens
Cell Transplantation
Hepatectomy
Genes
Infertility
Plasmids
Pathogens
Antigens
Viruses
Gene Expression
Gene expression
Rats

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Establishment of a highly differentiated immortalized human hepatocyte cell line with tight regulation. / Kobayashi, N.; Noguchi, H.; Fujiwara, Toshiyoshi; Tanaka, N.

In: ASAIO Journal, Vol. 46, No. 2, 03.2000, p. 229.

Research output: Contribution to journalArticle

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