Establishment of a highly differentiated immortalized human hepatocyte cell line with tight regulation

N. Kobayashi; H. Noguchi; T. Fujiwara; N. Tanaka

doi:10.1097/00002480-200003000-00312

Establishment of a highly differentiated immortalized human hepatocyte cell line with tight regulation

N. Kobayashi, H. Noguchi, T. Fujiwara, N. Tanaka

Research output: Contribution to journal › Conference article › peer-review

Abstract

To determine an ideal cell line for bioartificial liver (BAL) therapy, primary human hepatocytes were immortalized with a plasmid SV3neo containing the genes of simian virus 40 large T antigen and neomycin phosphototransferase by a method of lipofection. As a safeguard in clinical application, HSV-tk gene was retrovirally introduced into the immortal cells. The gene expression of liver-specific functions and in vitro sensitivity to ganciclovir (GCV) were examined. To investigate the in-vivo potential of cells, intrasplenic transplantation (Isp-Tx) was performed in rats that underwent 90% hepatectomy. The results indicate that the cells can offer the advantages of uniformity, sterility, unlimited availability, and freedom from infectious pathogens and may be useful for BAL.

Original language	English
Number of pages	1
Journal	ASAIO Journal
Volume	46
Issue number	2
DOIs	https://doi.org/10.1097/00002480-200003000-00312
Publication status	Published - Jan 1 2000
Event	46th Annual Conference and Exposition of ASAIO - New York, NY, USA Duration: Jun 28 2000 → Jul 1 2000

ASJC Scopus subject areas

Biophysics
Bioengineering
Biomaterials
Biomedical Engineering

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10.1097/00002480-200003000-00312

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title = "Establishment of a highly differentiated immortalized human hepatocyte cell line with tight regulation",

abstract = "To determine an ideal cell line for bioartificial liver (BAL) therapy, primary human hepatocytes were immortalized with a plasmid SV3neo containing the genes of simian virus 40 large T antigen and neomycin phosphototransferase by a method of lipofection. As a safeguard in clinical application, HSV-tk gene was retrovirally introduced into the immortal cells. The gene expression of liver-specific functions and in vitro sensitivity to ganciclovir (GCV) were examined. To investigate the in-vivo potential of cells, intrasplenic transplantation (Isp-Tx) was performed in rats that underwent 90% hepatectomy. The results indicate that the cells can offer the advantages of uniformity, sterility, unlimited availability, and freedom from infectious pathogens and may be useful for BAL.",

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T1 - Establishment of a highly differentiated immortalized human hepatocyte cell line with tight regulation

AU - Kobayashi, N.

AU - Noguchi, H.

AU - Fujiwara, T.

AU - Tanaka, N.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - To determine an ideal cell line for bioartificial liver (BAL) therapy, primary human hepatocytes were immortalized with a plasmid SV3neo containing the genes of simian virus 40 large T antigen and neomycin phosphototransferase by a method of lipofection. As a safeguard in clinical application, HSV-tk gene was retrovirally introduced into the immortal cells. The gene expression of liver-specific functions and in vitro sensitivity to ganciclovir (GCV) were examined. To investigate the in-vivo potential of cells, intrasplenic transplantation (Isp-Tx) was performed in rats that underwent 90% hepatectomy. The results indicate that the cells can offer the advantages of uniformity, sterility, unlimited availability, and freedom from infectious pathogens and may be useful for BAL.

AB - To determine an ideal cell line for bioartificial liver (BAL) therapy, primary human hepatocytes were immortalized with a plasmid SV3neo containing the genes of simian virus 40 large T antigen and neomycin phosphototransferase by a method of lipofection. As a safeguard in clinical application, HSV-tk gene was retrovirally introduced into the immortal cells. The gene expression of liver-specific functions and in vitro sensitivity to ganciclovir (GCV) were examined. To investigate the in-vivo potential of cells, intrasplenic transplantation (Isp-Tx) was performed in rats that underwent 90% hepatectomy. The results indicate that the cells can offer the advantages of uniformity, sterility, unlimited availability, and freedom from infectious pathogens and may be useful for BAL.

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Establishment of a highly differentiated immortalized human hepatocyte cell line with tight regulation

Abstract

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