To determine an ideal cell line for bioartificial liver (BAL) therapy, primary human hepatocytes were immortalized with a plasmid SV3neo containing the genes of simian virus 40 large T antigen and neomycin phosphototransferase by a method of lipofection. As a safeguard in clinical application, HSV-tk gene was retrovirally introduced into the immortal cells. The gene expression of liver-specific functions and in vitro sensitivity to ganciclovir (GCV) were examined. To investigate the in-vivo potential of cells, intrasplenic transplantation (Isp-Tx) was performed in rats that underwent 90% hepatectomy. The results indicate that the cells can offer the advantages of uniformity, sterility, unlimited availability, and freedom from infectious pathogens and may be useful for BAL.
|Number of pages||1|
|Publication status||Published - Jan 1 2000|
|Event||46th Annual Conference and Exposition of ASAIO - New York, NY, USA|
Duration: Jun 28 2000 → Jul 1 2000
ASJC Scopus subject areas
- Biomedical Engineering