Establishment and characterization of high- and low-lung-metastatic cell lines derived from murine colon adenocarcinoma 26 tumor line

Keita Sakata, Ken Ichi Kozaki, Ken Ichi Iida, Rie Tanaka, Sadako Yamagata, Kazuhiko R. Utsumi, Shinsuke Saga, Satoru Shimizu, Mutsushi Matsuyama

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We established and characterized high- (LuM1) and low-lung-metastatic (NM11) cell lines derived from murine colon adenocarcinoma 26 tumor line, LuM1 cell line was established as a clonal cell line from a cultured cell mixture derived from a lung-metastatic nodule after 7 sequential subcutaneous transplantations of lung-metastatic tumors in the abdominal wall of BALB/c mice. NM11 cell line was established from a cultured cell mixture derived from a subcutaneous transplant of murine colon adenocarcinoma 26 tumor cells. LuM1 cells showed marked spontaneous lung metastases, but NM11 cells rarely did. High invasive potential of LuM1 cells was revealed by in vitro invasion assay using Matrigel reconstituted membranes. Rapid retraction was observed in monolayers of human umbilical vein endothelial cells and bovine aortic endothelial cells when LuM1 cells were added on the monolayers. Gelatin zymography and immunochemical examinations with monoclonal antibodies against gelatinase B (M(r) 95,000 type IV collagenase) showed secretion of large amounts of the gelatinase by LuM1 cells.

Original languageEnglish
Pages (from-to)78-85
Number of pages8
JournalJapanese Journal of Cancer Research
Volume87
Issue number1
Publication statusPublished - Jan 1996
Externally publishedYes

Fingerprint

Colon
Adenocarcinoma
Cell Line
Lung
Neoplasms
Cultured Cells
Gelatinases
Lung Transplantation
Human Umbilical Vein Endothelial Cells
Matrix Metalloproteinase 9
Abdominal Wall
Collagenases
Gelatin
Endothelial Cells
Monoclonal Antibodies
Neoplasm Metastasis
Transplants
Membranes

Keywords

  • Basement membrane
  • Gelatinase B (M(r)95,000 type IV collagenase)
  • High-lung-metastatic cell line
  • LuM1
  • NM11

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sakata, K., Kozaki, K. I., Iida, K. I., Tanaka, R., Yamagata, S., Utsumi, K. R., ... Matsuyama, M. (1996). Establishment and characterization of high- and low-lung-metastatic cell lines derived from murine colon adenocarcinoma 26 tumor line. Japanese Journal of Cancer Research, 87(1), 78-85.

Establishment and characterization of high- and low-lung-metastatic cell lines derived from murine colon adenocarcinoma 26 tumor line. / Sakata, Keita; Kozaki, Ken Ichi; Iida, Ken Ichi; Tanaka, Rie; Yamagata, Sadako; Utsumi, Kazuhiko R.; Saga, Shinsuke; Shimizu, Satoru; Matsuyama, Mutsushi.

In: Japanese Journal of Cancer Research, Vol. 87, No. 1, 01.1996, p. 78-85.

Research output: Contribution to journalArticle

Sakata, K, Kozaki, KI, Iida, KI, Tanaka, R, Yamagata, S, Utsumi, KR, Saga, S, Shimizu, S & Matsuyama, M 1996, 'Establishment and characterization of high- and low-lung-metastatic cell lines derived from murine colon adenocarcinoma 26 tumor line', Japanese Journal of Cancer Research, vol. 87, no. 1, pp. 78-85.
Sakata, Keita ; Kozaki, Ken Ichi ; Iida, Ken Ichi ; Tanaka, Rie ; Yamagata, Sadako ; Utsumi, Kazuhiko R. ; Saga, Shinsuke ; Shimizu, Satoru ; Matsuyama, Mutsushi. / Establishment and characterization of high- and low-lung-metastatic cell lines derived from murine colon adenocarcinoma 26 tumor line. In: Japanese Journal of Cancer Research. 1996 ; Vol. 87, No. 1. pp. 78-85.
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