Establishment and characterization of an immature human megakaryoblastic cell line, MEG-A2

Akihiro Abe, Nobuhiko Emi, Hidefumi Kato, Koichi Adachi, Takashi Murate, Shinsuke Saga, Michinori Ogura, Tetsuhito Kojima, Mitsune Tanimoto, Norihisa Morishita, Kohei Kawashima, Hidehiko Saito

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15 Citations (Scopus)

Abstract

We have established a novel human megakaryoblastic cell line, designated as MEG-A2, from a patient with megakaryoblastic crisis of Philadelphia (Ph) chromosome positive chronic myelogenous leukemia. MEG-A2 cells showed positive phenotypes for periodic acid Schiff and α-naphthyl buthylate esterase reactions, but were negative for myeloperoxidase and naphthol ASD chloroacetate esterase reactions. Flow cytometric analyses of cell surface markers revealed that MEG-A2 cells had a low level of GP IIb/IIIa expression as well as apparent expressions of CD4, CD7, CD13, CD33 and CD34 antigens, but no expression of GP lb nor glycophorin A. Stimulation with phorbol 12-myristate 13-acetate (PMA) dramatically increased the expression of megakaryocyte-related markers such as HPL-3, J15, Plt-1, Y2/51 and AN51 in MEG-A2 cells. The PMA-stimulation also induced expression of platelet peroxidase (PPO) in MEG-A2 cells on electromicroscopic observation. Proliferative responses to granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) or erythropoietin were observed, and the expression of GP IIb/IIIa was increased by stimulation with GM-CSF, IL-3, erythropoietin and interleukin-6 (IL-6). Protein S mRNA expression was seen in cultured cells on Northern blot analysis. Expression of platelet factor 4 mRNA was induced in PMA-stimulated cells, and a marked accumulation of protein was observed in the culture medium. In conclusion, a new cell line, MEG-A2, belongs to the relatively immature megakaryocytic lineage and has markedly increased megakaryocytic characteristics with PMA stimulation.

Original languageEnglish
Pages (from-to)341-349
Number of pages9
JournalLeukemia
Volume9
Issue number2
Publication statusPublished - Feb 1995

Keywords

  • Cytokine
  • Megakaryoblastic cell line
  • PF4
  • PPO
  • Proteins

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Abe, A., Emi, N., Kato, H., Adachi, K., Murate, T., Saga, S., Ogura, M., Kojima, T., Tanimoto, M., Morishita, N., Kawashima, K., & Saito, H. (1995). Establishment and characterization of an immature human megakaryoblastic cell line, MEG-A2. Leukemia, 9(2), 341-349.