Establishment and characterization of an immature human megakaryoblastic cell line, MEG-A2

A. Abe, N. Emi, H. Kato, K. Adachi, T. Murate, S. Saga, M. Ogura, T. Kojima, M. Tanimoto, N. Morishita, K. Kawashima, H. Saito

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Abstract

We have established a novel human megakaryoblastic cell line, designated as MEG-A2, from a patient with megakaryoblastic crisis of Philadelphia (Ph) chromosome positive chronic myelogenous leukemia. MEG-A2 cells showed positive phenotypes for periodic acid Schiff and α-naphthyl buthylate esterase reactions, but were negative for myeloperoxidase and naphthol ASD chloroacetate esterase reactions. Flow cytometric analyses of cell surface markers revealed that MEG-A2 cells had a low level of GP IIb/IIIa expression as well as apparent expressions of CD4, CD7, CD13, CD33 and CD34 antigens, but no expression of GP lb nor glycophorin A. Stimulation with phorbol 12-myristate 13-acetate (PMA) dramatically increased the expression of megakaryocyte-related markers such as HPL-3, J15, Plt-1, Y2/51 and AN51 in MEG-A2 cells. The PMA-stimulation also induced expression of platelet peroxidase (PPO) in MEG-A2 cells on electromicroscopic observation. Proliferative responses to granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) or erythropoietin were observed, and the expression of GP IIb/IIIa was increased by stimulation with GM-CSF, IL-3, erythropoietin and interleukin-6 (IL-6). Protein S mRNA expression was seen in cultured cells on Northern blot analysis. Expression of platelet factor 4 mRNA was induced in PMA-stimulated cells, and a marked accumulation of protein was observed in the culture medium. In conclusion, a new cell line, MEG-A2, belongs to the relatively immature megakaryocytic lineage and has markedly increased megakaryocytic characteristics with PMA stimulation.

Original languageEnglish
Pages (from-to)341-349
Number of pages9
JournalLeukemia
Volume9
Issue number2
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

varespladib methyl
Cell Line
Acetates
Platelet Membrane Glycoprotein IIb
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Erythropoietin
Peroxidase
CD7 Antigens
Sialic Acid Binding Ig-like Lectin 3
CD34 Antigens
Glycophorin
CD13 Antigens
Platelet Factor 4
Naphthols
Philadelphia Chromosome
CD4 Antigens
Messenger RNA
Periodic Acid
Megakaryocytes

Keywords

  • Cytokine
  • Megakaryoblastic cell line
  • PF4
  • PPO
  • Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Abe, A., Emi, N., Kato, H., Adachi, K., Murate, T., Saga, S., ... Saito, H. (1995). Establishment and characterization of an immature human megakaryoblastic cell line, MEG-A2. Leukemia, 9(2), 341-349.

Establishment and characterization of an immature human megakaryoblastic cell line, MEG-A2. / Abe, A.; Emi, N.; Kato, H.; Adachi, K.; Murate, T.; Saga, S.; Ogura, M.; Kojima, T.; Tanimoto, M.; Morishita, N.; Kawashima, K.; Saito, H.

In: Leukemia, Vol. 9, No. 2, 1995, p. 341-349.

Research output: Contribution to journalArticle

Abe, A, Emi, N, Kato, H, Adachi, K, Murate, T, Saga, S, Ogura, M, Kojima, T, Tanimoto, M, Morishita, N, Kawashima, K & Saito, H 1995, 'Establishment and characterization of an immature human megakaryoblastic cell line, MEG-A2', Leukemia, vol. 9, no. 2, pp. 341-349.
Abe A, Emi N, Kato H, Adachi K, Murate T, Saga S et al. Establishment and characterization of an immature human megakaryoblastic cell line, MEG-A2. Leukemia. 1995;9(2):341-349.
Abe, A. ; Emi, N. ; Kato, H. ; Adachi, K. ; Murate, T. ; Saga, S. ; Ogura, M. ; Kojima, T. ; Tanimoto, M. ; Morishita, N. ; Kawashima, K. ; Saito, H. / Establishment and characterization of an immature human megakaryoblastic cell line, MEG-A2. In: Leukemia. 1995 ; Vol. 9, No. 2. pp. 341-349.
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abstract = "We have established a novel human megakaryoblastic cell line, designated as MEG-A2, from a patient with megakaryoblastic crisis of Philadelphia (Ph) chromosome positive chronic myelogenous leukemia. MEG-A2 cells showed positive phenotypes for periodic acid Schiff and α-naphthyl buthylate esterase reactions, but were negative for myeloperoxidase and naphthol ASD chloroacetate esterase reactions. Flow cytometric analyses of cell surface markers revealed that MEG-A2 cells had a low level of GP IIb/IIIa expression as well as apparent expressions of CD4, CD7, CD13, CD33 and CD34 antigens, but no expression of GP lb nor glycophorin A. Stimulation with phorbol 12-myristate 13-acetate (PMA) dramatically increased the expression of megakaryocyte-related markers such as HPL-3, J15, Plt-1, Y2/51 and AN51 in MEG-A2 cells. The PMA-stimulation also induced expression of platelet peroxidase (PPO) in MEG-A2 cells on electromicroscopic observation. Proliferative responses to granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) or erythropoietin were observed, and the expression of GP IIb/IIIa was increased by stimulation with GM-CSF, IL-3, erythropoietin and interleukin-6 (IL-6). Protein S mRNA expression was seen in cultured cells on Northern blot analysis. Expression of platelet factor 4 mRNA was induced in PMA-stimulated cells, and a marked accumulation of protein was observed in the culture medium. In conclusion, a new cell line, MEG-A2, belongs to the relatively immature megakaryocytic lineage and has markedly increased megakaryocytic characteristics with PMA stimulation.",
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AU - Abe, A.

AU - Emi, N.

AU - Kato, H.

AU - Adachi, K.

AU - Murate, T.

AU - Saga, S.

AU - Ogura, M.

AU - Kojima, T.

AU - Tanimoto, M.

AU - Morishita, N.

AU - Kawashima, K.

AU - Saito, H.

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N2 - We have established a novel human megakaryoblastic cell line, designated as MEG-A2, from a patient with megakaryoblastic crisis of Philadelphia (Ph) chromosome positive chronic myelogenous leukemia. MEG-A2 cells showed positive phenotypes for periodic acid Schiff and α-naphthyl buthylate esterase reactions, but were negative for myeloperoxidase and naphthol ASD chloroacetate esterase reactions. Flow cytometric analyses of cell surface markers revealed that MEG-A2 cells had a low level of GP IIb/IIIa expression as well as apparent expressions of CD4, CD7, CD13, CD33 and CD34 antigens, but no expression of GP lb nor glycophorin A. Stimulation with phorbol 12-myristate 13-acetate (PMA) dramatically increased the expression of megakaryocyte-related markers such as HPL-3, J15, Plt-1, Y2/51 and AN51 in MEG-A2 cells. The PMA-stimulation also induced expression of platelet peroxidase (PPO) in MEG-A2 cells on electromicroscopic observation. Proliferative responses to granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) or erythropoietin were observed, and the expression of GP IIb/IIIa was increased by stimulation with GM-CSF, IL-3, erythropoietin and interleukin-6 (IL-6). Protein S mRNA expression was seen in cultured cells on Northern blot analysis. Expression of platelet factor 4 mRNA was induced in PMA-stimulated cells, and a marked accumulation of protein was observed in the culture medium. In conclusion, a new cell line, MEG-A2, belongs to the relatively immature megakaryocytic lineage and has markedly increased megakaryocytic characteristics with PMA stimulation.

AB - We have established a novel human megakaryoblastic cell line, designated as MEG-A2, from a patient with megakaryoblastic crisis of Philadelphia (Ph) chromosome positive chronic myelogenous leukemia. MEG-A2 cells showed positive phenotypes for periodic acid Schiff and α-naphthyl buthylate esterase reactions, but were negative for myeloperoxidase and naphthol ASD chloroacetate esterase reactions. Flow cytometric analyses of cell surface markers revealed that MEG-A2 cells had a low level of GP IIb/IIIa expression as well as apparent expressions of CD4, CD7, CD13, CD33 and CD34 antigens, but no expression of GP lb nor glycophorin A. Stimulation with phorbol 12-myristate 13-acetate (PMA) dramatically increased the expression of megakaryocyte-related markers such as HPL-3, J15, Plt-1, Y2/51 and AN51 in MEG-A2 cells. The PMA-stimulation also induced expression of platelet peroxidase (PPO) in MEG-A2 cells on electromicroscopic observation. Proliferative responses to granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) or erythropoietin were observed, and the expression of GP IIb/IIIa was increased by stimulation with GM-CSF, IL-3, erythropoietin and interleukin-6 (IL-6). Protein S mRNA expression was seen in cultured cells on Northern blot analysis. Expression of platelet factor 4 mRNA was induced in PMA-stimulated cells, and a marked accumulation of protein was observed in the culture medium. In conclusion, a new cell line, MEG-A2, belongs to the relatively immature megakaryocytic lineage and has markedly increased megakaryocytic characteristics with PMA stimulation.

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