Abstract
A human acute lymphoblastic leukemia (ALL) cell line, BALM-18, was established from the peripheral blood specimen of a patient with B cell ALL L3 type (ALL-L3) at diagnosis using bone marrow stroma cells (BST) as feeder cells. The primary leukemia cells did not grow without feeder cells. As with the primary leukemia cells, BALM-18 showed an immunophenotype of Burkitt's lymphoma group I [CD10 +, CD20 +, CD23-, CD39-, CD77+] and carried the t(8;14)(q24;q32) chromosomal abnormality which is highly associated with ALL- L3 and Burkitt's lymphoma. It also revealed a significantly low level of bcl- 2 protein. Strikingly, anti-human IgM antibody did induce apoptosis in induction experiments. However, it was reversed by the addition of anti-CD40 antibody or BST cells, whereas the culture supernatant of the stroma cells did not show any effect on the inhibition of apoptosis. BALM-18 may be useful for analyzing both the mechanisms of anti-IgM induced apoptosis and signaling during the inhibition of apoptosis by CD40 or BST cells.
Original language | English |
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Pages (from-to) | 559-568 |
Number of pages | 10 |
Journal | Leukemia Research |
Volume | 23 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 1999 |
Keywords
- ALL-L3
- Anti-IgM
- Apoptosis
- Bone marrow stroma cells
- CD40
- Cell line
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research