Establishment and characterization of a novel ALL-L3 cell line (BALM- 18): Induction of apoptosis by anti-IgM and inhibition of apoptosis by bone marrow stroma cells

Yoshinobu Matsuo, Akira Sugimoto, Akira Harashima, Chiharu Nishizaki, Fumihiko Ishimaru, Eisei Kondo, Yoshio Katayama, Mine Harada, Kunzo Orita

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A human acute lymphoblastic leukemia (ALL) cell line, BALM-18, was established from the peripheral blood specimen of a patient with B cell ALL L3 type (ALL-L3) at diagnosis using bone marrow stroma cells (BST) as feeder cells. The primary leukemia cells did not grow without feeder cells. As with the primary leukemia cells, BALM-18 showed an immunophenotype of Burkitt's lymphoma group I [CD10 +, CD20 +, CD23-, CD39-, CD77+] and carried the t(8;14)(q24;q32) chromosomal abnormality which is highly associated with ALL- L3 and Burkitt's lymphoma. It also revealed a significantly low level of bcl- 2 protein. Strikingly, anti-human IgM antibody did induce apoptosis in induction experiments. However, it was reversed by the addition of anti-CD40 antibody or BST cells, whereas the culture supernatant of the stroma cells did not show any effect on the inhibition of apoptosis. BALM-18 may be useful for analyzing both the mechanisms of anti-IgM induced apoptosis and signaling during the inhibition of apoptosis by CD40 or BST cells.

Original languageEnglish
Pages (from-to)559-568
Number of pages10
JournalLeukemia Research
Volume23
Issue number6
DOIs
Publication statusPublished - Jun 1 1999

Keywords

  • ALL-L3
  • Anti-IgM
  • Apoptosis
  • Bone marrow stroma cells
  • CD40
  • Cell line

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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