TY - JOUR
T1 - Establishment and characterization of a novel ALL-L3 cell line (BALM- 18)
T2 - Induction of apoptosis by anti-IgM and inhibition of apoptosis by bone marrow stroma cells
AU - Matsuo, Yoshinobu
AU - Sugimoto, Akira
AU - Harashima, Akira
AU - Nishizaki, Chiharu
AU - Ishimaru, Fumihiko
AU - Kondo, Eisei
AU - Katayama, Yoshio
AU - Harada, Mine
AU - Orita, Kunzo
PY - 1999/6
Y1 - 1999/6
N2 - A human acute lymphoblastic leukemia (ALL) cell line, BALM-18, was established from the peripheral blood specimen of a patient with B cell ALL L3 type (ALL-L3) at diagnosis using bone marrow stroma cells (BST) as feeder cells. The primary leukemia cells did not grow without feeder cells. As with the primary leukemia cells, BALM-18 showed an immunophenotype of Burkitt's lymphoma group I [CD10 +, CD20 +, CD23-, CD39-, CD77+] and carried the t(8;14)(q24;q32) chromosomal abnormality which is highly associated with ALL- L3 and Burkitt's lymphoma. It also revealed a significantly low level of bcl- 2 protein. Strikingly, anti-human IgM antibody did induce apoptosis in induction experiments. However, it was reversed by the addition of anti-CD40 antibody or BST cells, whereas the culture supernatant of the stroma cells did not show any effect on the inhibition of apoptosis. BALM-18 may be useful for analyzing both the mechanisms of anti-IgM induced apoptosis and signaling during the inhibition of apoptosis by CD40 or BST cells.
AB - A human acute lymphoblastic leukemia (ALL) cell line, BALM-18, was established from the peripheral blood specimen of a patient with B cell ALL L3 type (ALL-L3) at diagnosis using bone marrow stroma cells (BST) as feeder cells. The primary leukemia cells did not grow without feeder cells. As with the primary leukemia cells, BALM-18 showed an immunophenotype of Burkitt's lymphoma group I [CD10 +, CD20 +, CD23-, CD39-, CD77+] and carried the t(8;14)(q24;q32) chromosomal abnormality which is highly associated with ALL- L3 and Burkitt's lymphoma. It also revealed a significantly low level of bcl- 2 protein. Strikingly, anti-human IgM antibody did induce apoptosis in induction experiments. However, it was reversed by the addition of anti-CD40 antibody or BST cells, whereas the culture supernatant of the stroma cells did not show any effect on the inhibition of apoptosis. BALM-18 may be useful for analyzing both the mechanisms of anti-IgM induced apoptosis and signaling during the inhibition of apoptosis by CD40 or BST cells.
KW - ALL-L3
KW - Anti-IgM
KW - Apoptosis
KW - Bone marrow stroma cells
KW - CD40
KW - Cell line
UR - http://www.scopus.com/inward/record.url?scp=0032998622&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032998622&partnerID=8YFLogxK
U2 - 10.1016/S0145-2126(99)00046-6
DO - 10.1016/S0145-2126(99)00046-6
M3 - Article
C2 - 10374849
AN - SCOPUS:0032998622
VL - 23
SP - 559
EP - 568
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 6
ER -
