Establishment and characterization of a murine mucosal mast cell culture model

Aya Kakinoki, Tsuyoshi Kameo, Shoko Yamashita, Kazuyuki Furuta, Satoshi Tanaka

Research output: Contribution to journalArticle

Abstract

Accumulating evidence suggests that mast cells play critical roles in disruption and maintenance of intestinal homeostasis, although it remains unknown how they affect the local microenvironment. Interleukin-9 (IL-9) was found to play critical roles in intestinal mast cell accumulation induced in various pathological conditions, such as parasite infection and oral allergen-induced anaphylaxis. Newly recruited intestinal mast cells trigger inflammatory responses and damage epithelial integrity through release of a wide variety of mediators including mast cell proteases. We established a novel culture model (IL-9-modified mast cells, MCs/IL-9), in which murine IL-3-dependent bone-marrow-derived cultured mast cells (BMMCs) were further cultured in the presence of stem cell factor and IL-9. In MCs/IL-9, drastic upregulation of Mcpt1 and Mcpt2 was found. Although histamine storage and tryptase activity were significantly downregulated in the presence of SCF and IL-9, this was entirely reversed when mast cells were cocultured with a murine fibroblastic cell line, Swiss 3T3. MCs/IL-9 underwent degranulation upon IgE-mediated antigen stimulation, which was found to less sensitive to lower concentrations of IgE in comparison with BMMCs. This model might be useful for investigation of the spatiotemporal changes of newly recruited intestinal mast cells.

Original languageEnglish
Article number236
JournalInternational journal of molecular sciences
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 1 2020

Fingerprint

Interleukin-9
interleukins
Cell culture
Mast Cells
Bone
Cell Culture Techniques
Allergens
Antigens
Stem cells
cultured cells
Cells
bone marrow
anaphylaxis
Immunoglobulin E
histamines
Cultured Cells
homeostasis
parasites
Tryptases
protease

Keywords

  • ATP
  • Chymase
  • Histamine
  • IL-9
  • Mast cell

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Establishment and characterization of a murine mucosal mast cell culture model. / Kakinoki, Aya; Kameo, Tsuyoshi; Yamashita, Shoko; Furuta, Kazuyuki; Tanaka, Satoshi.

In: International journal of molecular sciences, Vol. 21, No. 1, 236, 01.01.2020.

Research output: Contribution to journalArticle

Kakinoki, Aya ; Kameo, Tsuyoshi ; Yamashita, Shoko ; Furuta, Kazuyuki ; Tanaka, Satoshi. / Establishment and characterization of a murine mucosal mast cell culture model. In: International journal of molecular sciences. 2020 ; Vol. 21, No. 1.
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