Abstract
The characteristics of a new human clear cell sarcoma (CCSa) cell line, HS-MM, established from the pleural effusion in a 39-year-old man with lung metastasis, have been morphologically studied in vitro and in vivo. HS-MM cells growing on a cover-slip were round or spindle in shape with round nuclei containing extremely prominent nucleoli. Heterotransplantation of the cells into nude mice was easily succeeded following tumor development. Light microscopically, HS-MM cells, both in vitro and in vivo, were positive for anti-S-100 protein and anti-melanoma specific antibodies with immunostain, but no melanin pigment was detected in them. Ultrastructurally, the cells had round euchromatin-rich nuclei with large nucleoli revealing conspicuous nucleolonema, and contained a few mitochondria, rough endoplasmic reticulum and lysosomal dense bodies, besides a large amount of glycogen, but no melanosome in their cytoplasm. HS-MM cells retained and fully expressed morphologically unique characteristics as a CCSa, compatible with amelanotic type of malignant melanoma also. This cell line, HS-MM, therefore, proves to be extremely useful for clinicopathological studies on a CCSa.
| Original language | English |
|---|---|
| Pages (from-to) | 352-356 |
| Number of pages | 5 |
| Journal | Human cell : official journal of Human Cell Research Society |
| Volume | 3 |
| Issue number | 4 |
| Publication status | Published - Dec 1990 |
| Externally published | Yes |
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Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma. / Sonobe, H.; Manabe, Y.; Furihata, M.; Iwata, J.; Hikita, T.; Tanimoto, T.; Kiuna, O.; Oka, Takashi; Ohtsuki, Y.; Mizobuchi, H.
In: Human cell : official journal of Human Cell Research Society, Vol. 3, No. 4, 12.1990, p. 352-356.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma
AU - Sonobe, H.
AU - Manabe, Y.
AU - Furihata, M.
AU - Iwata, J.
AU - Hikita, T.
AU - Tanimoto, T.
AU - Kiuna, O.
AU - Oka, Takashi
AU - Ohtsuki, Y.
AU - Mizobuchi, H.
PY - 1990/12
Y1 - 1990/12
N2 - The characteristics of a new human clear cell sarcoma (CCSa) cell line, HS-MM, established from the pleural effusion in a 39-year-old man with lung metastasis, have been morphologically studied in vitro and in vivo. HS-MM cells growing on a cover-slip were round or spindle in shape with round nuclei containing extremely prominent nucleoli. Heterotransplantation of the cells into nude mice was easily succeeded following tumor development. Light microscopically, HS-MM cells, both in vitro and in vivo, were positive for anti-S-100 protein and anti-melanoma specific antibodies with immunostain, but no melanin pigment was detected in them. Ultrastructurally, the cells had round euchromatin-rich nuclei with large nucleoli revealing conspicuous nucleolonema, and contained a few mitochondria, rough endoplasmic reticulum and lysosomal dense bodies, besides a large amount of glycogen, but no melanosome in their cytoplasm. HS-MM cells retained and fully expressed morphologically unique characteristics as a CCSa, compatible with amelanotic type of malignant melanoma also. This cell line, HS-MM, therefore, proves to be extremely useful for clinicopathological studies on a CCSa.
AB - The characteristics of a new human clear cell sarcoma (CCSa) cell line, HS-MM, established from the pleural effusion in a 39-year-old man with lung metastasis, have been morphologically studied in vitro and in vivo. HS-MM cells growing on a cover-slip were round or spindle in shape with round nuclei containing extremely prominent nucleoli. Heterotransplantation of the cells into nude mice was easily succeeded following tumor development. Light microscopically, HS-MM cells, both in vitro and in vivo, were positive for anti-S-100 protein and anti-melanoma specific antibodies with immunostain, but no melanin pigment was detected in them. Ultrastructurally, the cells had round euchromatin-rich nuclei with large nucleoli revealing conspicuous nucleolonema, and contained a few mitochondria, rough endoplasmic reticulum and lysosomal dense bodies, besides a large amount of glycogen, but no melanosome in their cytoplasm. HS-MM cells retained and fully expressed morphologically unique characteristics as a CCSa, compatible with amelanotic type of malignant melanoma also. This cell line, HS-MM, therefore, proves to be extremely useful for clinicopathological studies on a CCSa.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025656432&partnerID=8YFLogxK
M3 - Article
C2 - 2095846
AN - SCOPUS:0025656432
VL - 3
SP - 352
EP - 356
JO - Human Cell
JF - Human Cell
SN - 0914-7470
IS - 4
ER -