Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma

H. Sonobe, Y. Manabe, M. Furihata, J. Iwata, T. Hikita, T. Tanimoto, O. Kiuna, Takashi Oka, Y. Ohtsuki, H. Mizobuchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The characteristics of a new human clear cell sarcoma (CCSa) cell line, HS-MM, established from the pleural effusion in a 39-year-old man with lung metastasis, have been morphologically studied in vitro and in vivo. HS-MM cells growing on a cover-slip were round or spindle in shape with round nuclei containing extremely prominent nucleoli. Heterotransplantation of the cells into nude mice was easily succeeded following tumor development. Light microscopically, HS-MM cells, both in vitro and in vivo, were positive for anti-S-100 protein and anti-melanoma specific antibodies with immunostain, but no melanin pigment was detected in them. Ultrastructurally, the cells had round euchromatin-rich nuclei with large nucleoli revealing conspicuous nucleolonema, and contained a few mitochondria, rough endoplasmic reticulum and lysosomal dense bodies, besides a large amount of glycogen, but no melanosome in their cytoplasm. HS-MM cells retained and fully expressed morphologically unique characteristics as a CCSa, compatible with amelanotic type of malignant melanoma also. This cell line, HS-MM, therefore, proves to be extremely useful for clinicopathological studies on a CCSa.

Original languageEnglish
Pages (from-to)352-356
Number of pages5
JournalHuman cell : official journal of Human Cell Research Society
Volume3
Issue number4
Publication statusPublished - Dec 1990
Externally publishedYes

Fingerprint

Clear Cell Sarcoma
Cell Line
Melanoma
Amelanotic Melanoma
Euchromatin
Melanosomes
S100 Proteins
Rough Endoplasmic Reticulum
Melanins
Pleural Effusion
Glycogen
Nude Mice
Mitochondria
Cytoplasm
Neoplasm Metastasis
Light
Lung
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sonobe, H., Manabe, Y., Furihata, M., Iwata, J., Hikita, T., Tanimoto, T., ... Mizobuchi, H. (1990). Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma. Human cell : official journal of Human Cell Research Society, 3(4), 352-356.

Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma. / Sonobe, H.; Manabe, Y.; Furihata, M.; Iwata, J.; Hikita, T.; Tanimoto, T.; Kiuna, O.; Oka, Takashi; Ohtsuki, Y.; Mizobuchi, H.

In: Human cell : official journal of Human Cell Research Society, Vol. 3, No. 4, 12.1990, p. 352-356.

Research output: Contribution to journalArticle

Sonobe, H, Manabe, Y, Furihata, M, Iwata, J, Hikita, T, Tanimoto, T, Kiuna, O, Oka, T, Ohtsuki, Y & Mizobuchi, H 1990, 'Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma', Human cell : official journal of Human Cell Research Society, vol. 3, no. 4, pp. 352-356.
Sonobe, H. ; Manabe, Y. ; Furihata, M. ; Iwata, J. ; Hikita, T. ; Tanimoto, T. ; Kiuna, O. ; Oka, Takashi ; Ohtsuki, Y. ; Mizobuchi, H. / Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma. In: Human cell : official journal of Human Cell Research Society. 1990 ; Vol. 3, No. 4. pp. 352-356.
@article{9892b1073aa24d2181e9bd2571bcfd17,
title = "Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma",
abstract = "The characteristics of a new human clear cell sarcoma (CCSa) cell line, HS-MM, established from the pleural effusion in a 39-year-old man with lung metastasis, have been morphologically studied in vitro and in vivo. HS-MM cells growing on a cover-slip were round or spindle in shape with round nuclei containing extremely prominent nucleoli. Heterotransplantation of the cells into nude mice was easily succeeded following tumor development. Light microscopically, HS-MM cells, both in vitro and in vivo, were positive for anti-S-100 protein and anti-melanoma specific antibodies with immunostain, but no melanin pigment was detected in them. Ultrastructurally, the cells had round euchromatin-rich nuclei with large nucleoli revealing conspicuous nucleolonema, and contained a few mitochondria, rough endoplasmic reticulum and lysosomal dense bodies, besides a large amount of glycogen, but no melanosome in their cytoplasm. HS-MM cells retained and fully expressed morphologically unique characteristics as a CCSa, compatible with amelanotic type of malignant melanoma also. This cell line, HS-MM, therefore, proves to be extremely useful for clinicopathological studies on a CCSa.",
author = "H. Sonobe and Y. Manabe and M. Furihata and J. Iwata and T. Hikita and T. Tanimoto and O. Kiuna and Takashi Oka and Y. Ohtsuki and H. Mizobuchi",
year = "1990",
month = "12",
language = "English",
volume = "3",
pages = "352--356",
journal = "Human Cell",
issn = "0914-7470",
publisher = "Springer Heidelberg",
number = "4",

}

TY - JOUR

T1 - Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma

AU - Sonobe, H.

AU - Manabe, Y.

AU - Furihata, M.

AU - Iwata, J.

AU - Hikita, T.

AU - Tanimoto, T.

AU - Kiuna, O.

AU - Oka, Takashi

AU - Ohtsuki, Y.

AU - Mizobuchi, H.

PY - 1990/12

Y1 - 1990/12

N2 - The characteristics of a new human clear cell sarcoma (CCSa) cell line, HS-MM, established from the pleural effusion in a 39-year-old man with lung metastasis, have been morphologically studied in vitro and in vivo. HS-MM cells growing on a cover-slip were round or spindle in shape with round nuclei containing extremely prominent nucleoli. Heterotransplantation of the cells into nude mice was easily succeeded following tumor development. Light microscopically, HS-MM cells, both in vitro and in vivo, were positive for anti-S-100 protein and anti-melanoma specific antibodies with immunostain, but no melanin pigment was detected in them. Ultrastructurally, the cells had round euchromatin-rich nuclei with large nucleoli revealing conspicuous nucleolonema, and contained a few mitochondria, rough endoplasmic reticulum and lysosomal dense bodies, besides a large amount of glycogen, but no melanosome in their cytoplasm. HS-MM cells retained and fully expressed morphologically unique characteristics as a CCSa, compatible with amelanotic type of malignant melanoma also. This cell line, HS-MM, therefore, proves to be extremely useful for clinicopathological studies on a CCSa.

AB - The characteristics of a new human clear cell sarcoma (CCSa) cell line, HS-MM, established from the pleural effusion in a 39-year-old man with lung metastasis, have been morphologically studied in vitro and in vivo. HS-MM cells growing on a cover-slip were round or spindle in shape with round nuclei containing extremely prominent nucleoli. Heterotransplantation of the cells into nude mice was easily succeeded following tumor development. Light microscopically, HS-MM cells, both in vitro and in vivo, were positive for anti-S-100 protein and anti-melanoma specific antibodies with immunostain, but no melanin pigment was detected in them. Ultrastructurally, the cells had round euchromatin-rich nuclei with large nucleoli revealing conspicuous nucleolonema, and contained a few mitochondria, rough endoplasmic reticulum and lysosomal dense bodies, besides a large amount of glycogen, but no melanosome in their cytoplasm. HS-MM cells retained and fully expressed morphologically unique characteristics as a CCSa, compatible with amelanotic type of malignant melanoma also. This cell line, HS-MM, therefore, proves to be extremely useful for clinicopathological studies on a CCSa.

UR - http://www.scopus.com/inward/record.url?scp=0025656432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025656432&partnerID=8YFLogxK

M3 - Article

C2 - 2095846

AN - SCOPUS:0025656432

VL - 3

SP - 352

EP - 356

JO - Human Cell

JF - Human Cell

SN - 0914-7470

IS - 4

ER -