Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1

Akira Kawai, Noriko Naito, Aki Yoshida, Yuki Morimoto, Mamoru Oouchida, Kenji Shimizu, Yasuo Beppu

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We describe here the establishment of a new synovial sarcoma cell line, SYO-1, derived from a biphasic synovial sarcoma that developed in the groin of a 19-year-old female. The cell line was maintained for more than 70 passages (more than 24 months) in vitro. The SYO-1 cells in monolayer culture exhibited a spindle shape without conspicuous pleomorphism. Immunohistochemically, the cells were positive for vimentin, type IV collagen, S-100, mdm2, bcl-2, c-Met and c-Kit. Tumors developed by their implantation in nude mice histologically showed biphasic features that were composed of areas of fascicles of spindle cells and areas of compact proliferation of polygonal to ovoid cells, which occasionally formed epithelial plaque and expressed cytokeratin and EMA. SYO-1 cells harbored the characteristic t(X;18)(p11.2;q11.2) translocation by chromosome analysis and SYT-SSX2 chimeric transcript by RT-PCR. The SYO-1 cells, the first characterized cell line derived from biphasic synovial sarcoma retaining the characteristic genetic and phenotypic features of the tumor, will be useful for various investigations on synovial sarcoma, especially for its epithelial differentiation.

Original languageEnglish
Pages (from-to)105-113
Number of pages9
JournalCancer Letters
Volume204
Issue number1
DOIs
Publication statusPublished - Feb 10 2004

Fingerprint

Synovial Sarcoma
Cell Line
Collagen Type IV
Groin
Vimentin
Keratins
Nude Mice
Neoplasms
Chromosomes
Polymerase Chain Reaction

Keywords

  • Biphasic
  • Cell line
  • Epithelial differentiation
  • Synovial sarcoma
  • SYT-SSX

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Kawai, A., Naito, N., Yoshida, A., Morimoto, Y., Oouchida, M., Shimizu, K., & Beppu, Y. (2004). Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1. Cancer Letters, 204(1), 105-113. https://doi.org/10.1016/j.canlet.2003.09.031

Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1. / Kawai, Akira; Naito, Noriko; Yoshida, Aki; Morimoto, Yuki; Oouchida, Mamoru; Shimizu, Kenji; Beppu, Yasuo.

In: Cancer Letters, Vol. 204, No. 1, 10.02.2004, p. 105-113.

Research output: Contribution to journalArticle

Kawai, A, Naito, N, Yoshida, A, Morimoto, Y, Oouchida, M, Shimizu, K & Beppu, Y 2004, 'Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1', Cancer Letters, vol. 204, no. 1, pp. 105-113. https://doi.org/10.1016/j.canlet.2003.09.031
Kawai, Akira ; Naito, Noriko ; Yoshida, Aki ; Morimoto, Yuki ; Oouchida, Mamoru ; Shimizu, Kenji ; Beppu, Yasuo. / Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1. In: Cancer Letters. 2004 ; Vol. 204, No. 1. pp. 105-113.
@article{f4bc36c88e4341a1803856a61719d562,
title = "Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1",
abstract = "We describe here the establishment of a new synovial sarcoma cell line, SYO-1, derived from a biphasic synovial sarcoma that developed in the groin of a 19-year-old female. The cell line was maintained for more than 70 passages (more than 24 months) in vitro. The SYO-1 cells in monolayer culture exhibited a spindle shape without conspicuous pleomorphism. Immunohistochemically, the cells were positive for vimentin, type IV collagen, S-100, mdm2, bcl-2, c-Met and c-Kit. Tumors developed by their implantation in nude mice histologically showed biphasic features that were composed of areas of fascicles of spindle cells and areas of compact proliferation of polygonal to ovoid cells, which occasionally formed epithelial plaque and expressed cytokeratin and EMA. SYO-1 cells harbored the characteristic t(X;18)(p11.2;q11.2) translocation by chromosome analysis and SYT-SSX2 chimeric transcript by RT-PCR. The SYO-1 cells, the first characterized cell line derived from biphasic synovial sarcoma retaining the characteristic genetic and phenotypic features of the tumor, will be useful for various investigations on synovial sarcoma, especially for its epithelial differentiation.",
keywords = "Biphasic, Cell line, Epithelial differentiation, Synovial sarcoma, SYT-SSX",
author = "Akira Kawai and Noriko Naito and Aki Yoshida and Yuki Morimoto and Mamoru Oouchida and Kenji Shimizu and Yasuo Beppu",
year = "2004",
month = "2",
day = "10",
doi = "10.1016/j.canlet.2003.09.031",
language = "English",
volume = "204",
pages = "105--113",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1

AU - Kawai, Akira

AU - Naito, Noriko

AU - Yoshida, Aki

AU - Morimoto, Yuki

AU - Oouchida, Mamoru

AU - Shimizu, Kenji

AU - Beppu, Yasuo

PY - 2004/2/10

Y1 - 2004/2/10

N2 - We describe here the establishment of a new synovial sarcoma cell line, SYO-1, derived from a biphasic synovial sarcoma that developed in the groin of a 19-year-old female. The cell line was maintained for more than 70 passages (more than 24 months) in vitro. The SYO-1 cells in monolayer culture exhibited a spindle shape without conspicuous pleomorphism. Immunohistochemically, the cells were positive for vimentin, type IV collagen, S-100, mdm2, bcl-2, c-Met and c-Kit. Tumors developed by their implantation in nude mice histologically showed biphasic features that were composed of areas of fascicles of spindle cells and areas of compact proliferation of polygonal to ovoid cells, which occasionally formed epithelial plaque and expressed cytokeratin and EMA. SYO-1 cells harbored the characteristic t(X;18)(p11.2;q11.2) translocation by chromosome analysis and SYT-SSX2 chimeric transcript by RT-PCR. The SYO-1 cells, the first characterized cell line derived from biphasic synovial sarcoma retaining the characteristic genetic and phenotypic features of the tumor, will be useful for various investigations on synovial sarcoma, especially for its epithelial differentiation.

AB - We describe here the establishment of a new synovial sarcoma cell line, SYO-1, derived from a biphasic synovial sarcoma that developed in the groin of a 19-year-old female. The cell line was maintained for more than 70 passages (more than 24 months) in vitro. The SYO-1 cells in monolayer culture exhibited a spindle shape without conspicuous pleomorphism. Immunohistochemically, the cells were positive for vimentin, type IV collagen, S-100, mdm2, bcl-2, c-Met and c-Kit. Tumors developed by their implantation in nude mice histologically showed biphasic features that were composed of areas of fascicles of spindle cells and areas of compact proliferation of polygonal to ovoid cells, which occasionally formed epithelial plaque and expressed cytokeratin and EMA. SYO-1 cells harbored the characteristic t(X;18)(p11.2;q11.2) translocation by chromosome analysis and SYT-SSX2 chimeric transcript by RT-PCR. The SYO-1 cells, the first characterized cell line derived from biphasic synovial sarcoma retaining the characteristic genetic and phenotypic features of the tumor, will be useful for various investigations on synovial sarcoma, especially for its epithelial differentiation.

KW - Biphasic

KW - Cell line

KW - Epithelial differentiation

KW - Synovial sarcoma

KW - SYT-SSX

UR - http://www.scopus.com/inward/record.url?scp=1642564269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642564269&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2003.09.031

DO - 10.1016/j.canlet.2003.09.031

M3 - Article

C2 - 14744540

AN - SCOPUS:1642564269

VL - 204

SP - 105

EP - 113

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -