Essential roles of the winged helix transcription factor MFH-1 in aortic arch patterning and skeletogenesis

Kiyoshi Iida, Haruhiko Koseki, Hideaki Kakinuma, Naoko Kato, Yoko Mizutani-Koseki, Hideyo Ohuchi, Hidefumi Yoshioka, Sumihare Noji, Koichi Kawamura, Yuki Kataoka, Fukuko Ueno, Masaru Taniguchi, Nobuaki Yoshida, Toshihiro Sugiyama, Naoyuki Miura

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

Mesenchyme Fork Head-1 (MFH-1) is a forkhead (also called winged helix) transcription factor defined by a common 100-amino acid DNA-binding domain. MFH-1 is expressed in non-notochordal mesoderm in the prospective trunk region and in cephalic neural-crest and cephalic mesoderm-derived mesenchymal cells in the prechordal region of early embryos. Subsequently, strong expression is localized in developing cartilaginous tissues, kidney and dorsal aortas. To investigate the developmental roles of MFH-1 during embryogenesis, mice lacking the MFH-1 locus were generated by targeted mutagenesis. MFH-1-deficient mice died embryonically and perinatally, and exhibited interrupted aortic arch and skeletal defects in the neurocranium and the vertebral column. Interruption of the aortic arch seen in the mutant mice was the same as in human congenital anomalies. These results suggest that MFH-1 has indispensable roles during the extensive remodeling of the aortic arch in neural-crest-derived cells and in skeletogenesis in cells derived from the neural crest and the mesoderm.

Original languageEnglish
Pages (from-to)4627-4638
Number of pages12
JournalDevelopment
Volume124
Issue number22
Publication statusPublished - Dec 17 1997
Externally publishedYes

Keywords

  • Aortic arch
  • DiGeorge syndrome
  • Interruption
  • Knockout mouse
  • MFH-1
  • Neural crest cells
  • Skeletogenesis
  • Winged helix proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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  • Cite this

    Iida, K., Koseki, H., Kakinuma, H., Kato, N., Mizutani-Koseki, Y., Ohuchi, H., Yoshioka, H., Noji, S., Kawamura, K., Kataoka, Y., Ueno, F., Taniguchi, M., Yoshida, N., Sugiyama, T., & Miura, N. (1997). Essential roles of the winged helix transcription factor MFH-1 in aortic arch patterning and skeletogenesis. Development, 124(22), 4627-4638.