Escherichia coli YaeJ protein mediates a novel ribosome-rescue pathway distinct from SsrA- and ArfA-mediated pathways

Yuhei Chadani, Katsuhiko Ono, Kazuhiro Kutsukake, Tatsuhiko Abo

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Accumulation of stalled ribosomes at the 3' end of mRNA without a stop codon (non-stop mRNA) is supposed to be toxic to bacterial cells. Escherichia coli has at least two distinct systems to rescue such stalled ribosomes: SsrA-dependent trans-translation and ArfA-dependent ribosome rescue. Combination of the ssrA and arfA mutations is synthetically lethal, suggesting the significance of ribosome rescue. In this study, we identified the E. coli yaeJ gene, encoding a peptide-release factor homologue with GGQ motif, as a multicopy suppressor of the lethal phenotype of ssrA arfA double mutant. The YaeJ protein was shown to bind to ribosomes. Both in vivo and in vitro, YaeJ showed the ribosome-rescue activity and promoted the hydrolysis of peptidyl-tRNA residing in the stalled ribosome. Missense mutation in the GGQ motif or deletion of the C-terminal unstructured tail abolished both the suppressor activity for ssrA arfA synthetic lethality and the ribosome-rescue activity, suggesting the importance of these structural features. On the basis of these observations, we propose that YaeJ acts as a stop codon-independent peptidyl-tRNA hydrolysing factor through binding to ribosomes stalled at the 3' end of non-stop mRNAs. It was also suggested that ArfA and YaeJ rescue the stalled ribosomes by distinct mechanisms.

Original languageEnglish
Pages (from-to)772-785
Number of pages14
JournalMolecular Microbiology
Volume80
Issue number3
DOIs
Publication statusPublished - May 2011

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Escherichia coli Proteins
Ribosomes
Terminator Codon
Messenger RNA
Escherichia coli
Poisons
Missense Mutation
Hydrolysis
Phenotype
Peptides
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Escherichia coli YaeJ protein mediates a novel ribosome-rescue pathway distinct from SsrA- and ArfA-mediated pathways. / Chadani, Yuhei; Ono, Katsuhiko; Kutsukake, Kazuhiro; Abo, Tatsuhiko.

In: Molecular Microbiology, Vol. 80, No. 3, 05.2011, p. 772-785.

Research output: Contribution to journalArticle

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