Escherichia coli pyruvate: Flavodoxin oxidoreductase, YdbK - Regulation of expression and biological roles in protection against oxidative stress

Takayuki Nakayama, Shinichirou Yonekura, Shuji Yonei, Qiu Mei Zhang-Akiyama

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

E. coli YdbK is predicted to be a pyruvate:flavodoxin oxidoreductase (PFOR). However, enzymatic activity and the regulation of gene expression of it are not well understood. In this study, we found that E. coli cells overexpressing the ydbK gene had enhanced PFOR activity, indicating the product of ydbK to be a PFOR. The PFOR was labile to oxygen. The expression of ydbK was induced by superoxide generators such as methyl viologen (MV) in a SoxS-dependent manner after a lag period. We identified a critical element upstream of ydbK gene required for the induction by MV and proved direct binding of SoxS to the element. E. coli ydbK mutant was highly sensitive to MV, which was enhanced by additional inactivation of fpr gene encoding ferredoxin (flavodoxin):NADP(H) reductase (FPR). Aconitase activity, a superoxide sensor, was more extensively decreased by MV in the E. coli ydbK mutant than in wild-type strain. The induction level of soxS gene was higher in E. coli ydbK fpr double mutant than in wildtype strain. These results indicate that YdbK helps to protect cells from oxidative stress. It is possible that YdbK maintains the cellular redox state together with FPR and is involved in the reduction of oxidized proteins including SoxR in the late stages of the oxidative stress response in E. coli.

Original languageEnglish
Pages (from-to)175-188
Number of pages14
JournalGenes and Genetic Systems
Volume88
Issue number3
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Paraquat
Oxidative Stress
Escherichia coli
Superoxides
Flavodoxin
Aconitate Hydratase
Genes
Ferredoxins
Gene Expression Regulation
Gene Silencing
NADP
Oxidation-Reduction
pyruvate-flavodoxin oxidoreductase
Oxidoreductases
Oxygen
Proteins

Keywords

  • Oxidative stress
  • Pyruvate: Flavodoxin oxidoreductase
  • SoxRS regulon
  • Superoxide inducible genes
  • YdbK

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Escherichia coli pyruvate : Flavodoxin oxidoreductase, YdbK - Regulation of expression and biological roles in protection against oxidative stress. / Nakayama, Takayuki; Yonekura, Shinichirou; Yonei, Shuji; Zhang-Akiyama, Qiu Mei.

In: Genes and Genetic Systems, Vol. 88, No. 3, 2013, p. 175-188.

Research output: Contribution to journalArticle

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