Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The induction of leukemic cell differentiation is a hopeful therapeutic modality. We studied the effects of monochloramine (NH2Cl) on erythroleukemic K562 cell differentiation, and compared the effects observed with those of U0126 and staurosporine, which are known inducers of erythroid and megakaryocytic differentiation, respectively. CD235 (glycophorin) expression, a marker of erythroid differentiation, was significantly increased by NH2Cl and U0126, along with an increase in cd235 mRNA levels. Other erythroid markers such as γ-globin and CD71 (transferrin receptor) were also increased by NH2Cl and U0126. In contrast, CD61 (integrin β3) and CD42b (GP1bα) expression, markers of megakaryocytic differentiation, was increased by staurosporine, but did not change significantly by NH2Cl and U0126. NH2Cl retarded cell proliferation without a marked loss of viability. When ERK phosphorylation (T202/Y204) and CD235 expression were compared using various chemicals, a strong negative correlation was observed (r = -0.76). Paradoxically, NH2Cl and staurosporine, but not U0126, induced large cells with multiple or lobulated nuclei, which was characteristic to megakaryocytes. NH2Cl increased the mRNA levels of gata1 and scl, decreased that of gata2, and did not change those of pu.1 and klf1. The changes observed in mRNA expression were different from those of U0126 or staurosporine. These results suggest that NH2Cl induces the bidirectional differentiation of K562. Oxidative stress may be effective in inducing leukemic cell differentiation.

Original languageEnglish
Pages (from-to)292-302
Number of pages11
JournalFree Radical Research
Volume48
Issue number3
DOIs
Publication statusPublished - Mar 2014

Fingerprint

K562 Cells
Staurosporine
Cell Differentiation
Differentiation Antigens
Messenger RNA
Glycophorin
Phosphorylation
Transferrin Receptors
Oxidative stress
Megakaryocytes
Globins
Cell proliferation
Integrins
U 0126
chloramine
Oxidative Stress
Cells
Cell Proliferation

Keywords

  • Cell differentiation
  • Extracellular signal-regulated kinase
  • Glycophorin
  • Integrin β3
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine. / Ogino, T.; Kobuchi, Hirotsugu; Fujita, Hirofumi; Matsukawa, Akihiro; Utsumi, K.

In: Free Radical Research, Vol. 48, No. 3, 03.2014, p. 292-302.

Research output: Contribution to journalArticle

@article{39a971dd2afc41548b92e80024a58288,
title = "Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine",
abstract = "The induction of leukemic cell differentiation is a hopeful therapeutic modality. We studied the effects of monochloramine (NH2Cl) on erythroleukemic K562 cell differentiation, and compared the effects observed with those of U0126 and staurosporine, which are known inducers of erythroid and megakaryocytic differentiation, respectively. CD235 (glycophorin) expression, a marker of erythroid differentiation, was significantly increased by NH2Cl and U0126, along with an increase in cd235 mRNA levels. Other erythroid markers such as γ-globin and CD71 (transferrin receptor) were also increased by NH2Cl and U0126. In contrast, CD61 (integrin β3) and CD42b (GP1bα) expression, markers of megakaryocytic differentiation, was increased by staurosporine, but did not change significantly by NH2Cl and U0126. NH2Cl retarded cell proliferation without a marked loss of viability. When ERK phosphorylation (T202/Y204) and CD235 expression were compared using various chemicals, a strong negative correlation was observed (r = -0.76). Paradoxically, NH2Cl and staurosporine, but not U0126, induced large cells with multiple or lobulated nuclei, which was characteristic to megakaryocytes. NH2Cl increased the mRNA levels of gata1 and scl, decreased that of gata2, and did not change those of pu.1 and klf1. The changes observed in mRNA expression were different from those of U0126 or staurosporine. These results suggest that NH2Cl induces the bidirectional differentiation of K562. Oxidative stress may be effective in inducing leukemic cell differentiation.",
keywords = "Cell differentiation, Extracellular signal-regulated kinase, Glycophorin, Integrin β3, Oxidative stress",
author = "T. Ogino and Hirotsugu Kobuchi and Hirofumi Fujita and Akihiro Matsukawa and K. Utsumi",
year = "2014",
month = "3",
doi = "10.3109/10715762.2013.865840",
language = "English",
volume = "48",
pages = "292--302",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine

AU - Ogino, T.

AU - Kobuchi, Hirotsugu

AU - Fujita, Hirofumi

AU - Matsukawa, Akihiro

AU - Utsumi, K.

PY - 2014/3

Y1 - 2014/3

N2 - The induction of leukemic cell differentiation is a hopeful therapeutic modality. We studied the effects of monochloramine (NH2Cl) on erythroleukemic K562 cell differentiation, and compared the effects observed with those of U0126 and staurosporine, which are known inducers of erythroid and megakaryocytic differentiation, respectively. CD235 (glycophorin) expression, a marker of erythroid differentiation, was significantly increased by NH2Cl and U0126, along with an increase in cd235 mRNA levels. Other erythroid markers such as γ-globin and CD71 (transferrin receptor) were also increased by NH2Cl and U0126. In contrast, CD61 (integrin β3) and CD42b (GP1bα) expression, markers of megakaryocytic differentiation, was increased by staurosporine, but did not change significantly by NH2Cl and U0126. NH2Cl retarded cell proliferation without a marked loss of viability. When ERK phosphorylation (T202/Y204) and CD235 expression were compared using various chemicals, a strong negative correlation was observed (r = -0.76). Paradoxically, NH2Cl and staurosporine, but not U0126, induced large cells with multiple or lobulated nuclei, which was characteristic to megakaryocytes. NH2Cl increased the mRNA levels of gata1 and scl, decreased that of gata2, and did not change those of pu.1 and klf1. The changes observed in mRNA expression were different from those of U0126 or staurosporine. These results suggest that NH2Cl induces the bidirectional differentiation of K562. Oxidative stress may be effective in inducing leukemic cell differentiation.

AB - The induction of leukemic cell differentiation is a hopeful therapeutic modality. We studied the effects of monochloramine (NH2Cl) on erythroleukemic K562 cell differentiation, and compared the effects observed with those of U0126 and staurosporine, which are known inducers of erythroid and megakaryocytic differentiation, respectively. CD235 (glycophorin) expression, a marker of erythroid differentiation, was significantly increased by NH2Cl and U0126, along with an increase in cd235 mRNA levels. Other erythroid markers such as γ-globin and CD71 (transferrin receptor) were also increased by NH2Cl and U0126. In contrast, CD61 (integrin β3) and CD42b (GP1bα) expression, markers of megakaryocytic differentiation, was increased by staurosporine, but did not change significantly by NH2Cl and U0126. NH2Cl retarded cell proliferation without a marked loss of viability. When ERK phosphorylation (T202/Y204) and CD235 expression were compared using various chemicals, a strong negative correlation was observed (r = -0.76). Paradoxically, NH2Cl and staurosporine, but not U0126, induced large cells with multiple or lobulated nuclei, which was characteristic to megakaryocytes. NH2Cl increased the mRNA levels of gata1 and scl, decreased that of gata2, and did not change those of pu.1 and klf1. The changes observed in mRNA expression were different from those of U0126 or staurosporine. These results suggest that NH2Cl induces the bidirectional differentiation of K562. Oxidative stress may be effective in inducing leukemic cell differentiation.

KW - Cell differentiation

KW - Extracellular signal-regulated kinase

KW - Glycophorin

KW - Integrin β3

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=84893594597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893594597&partnerID=8YFLogxK

U2 - 10.3109/10715762.2013.865840

DO - 10.3109/10715762.2013.865840

M3 - Article

C2 - 24237253

AN - SCOPUS:84893594597

VL - 48

SP - 292

EP - 302

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 3

ER -