Ergothioneine production using Methylobacterium species, yeast, and fungi

Yoshiko Fujitani, Kabir Md Alamgir, Akio Tani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains.

Original languageEnglish
Pages (from-to)715-722
Number of pages8
JournalJournal of Bioscience and Bioengineering
Volume126
Issue number6
DOIs
Publication statusPublished - Dec 2018

Fingerprint

Ergothioneine
Methylobacterium
Fungi
Yeast
Bacteria
Genes
Yeasts
Microorganisms
Amino acids
Ammonia
Screening
Animals
Sulfur
Health
Costs
Histidine Ammonia-Lyase
Rhodotorula
Weights and Measures
Histidine

Keywords

  • Anti-oxidative amino acid
  • Aureobasidium pullulans
  • Ergothioneine
  • Methanol
  • Methylobacterium species
  • Rhodotorula mucilaginosa

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Ergothioneine production using Methylobacterium species, yeast, and fungi. / Fujitani, Yoshiko; Alamgir, Kabir Md; Tani, Akio.

In: Journal of Bioscience and Bioengineering, Vol. 126, No. 6, 12.2018, p. 715-722.

Research output: Contribution to journalArticle

Fujitani, Yoshiko ; Alamgir, Kabir Md ; Tani, Akio. / Ergothioneine production using Methylobacterium species, yeast, and fungi. In: Journal of Bioscience and Bioengineering. 2018 ; Vol. 126, No. 6. pp. 715-722.
@article{b97d36ba602d4d559ed9683a450f33dd,
title = "Ergothioneine production using Methylobacterium species, yeast, and fungi",
abstract = "Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains.",
keywords = "Anti-oxidative amino acid, Aureobasidium pullulans, Ergothioneine, Methanol, Methylobacterium species, Rhodotorula mucilaginosa",
author = "Yoshiko Fujitani and Alamgir, {Kabir Md} and Akio Tani",
note = "Copyright {\circledC} 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.",
year = "2018",
month = "12",
doi = "10.1016/j.jbiosc.2018.05.021",
language = "English",
volume = "126",
pages = "715--722",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "6",

}

TY - JOUR

T1 - Ergothioneine production using Methylobacterium species, yeast, and fungi

AU - Fujitani, Yoshiko

AU - Alamgir, Kabir Md

AU - Tani, Akio

N1 - Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

PY - 2018/12

Y1 - 2018/12

N2 - Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains.

AB - Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains.

KW - Anti-oxidative amino acid

KW - Aureobasidium pullulans

KW - Ergothioneine

KW - Methanol

KW - Methylobacterium species

KW - Rhodotorula mucilaginosa

UR - http://www.scopus.com/inward/record.url?scp=85048446420&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048446420&partnerID=8YFLogxK

U2 - 10.1016/j.jbiosc.2018.05.021

DO - 10.1016/j.jbiosc.2018.05.021

M3 - Article

C2 - 29910189

AN - SCOPUS:85048446420

VL - 126

SP - 715

EP - 722

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 6

ER -