Eradication of melanoma in vitro and in vivo via targeting with a Killer-Red-containing telomerase-dependent adenovirus

Kiyoto Takehara, Shuuya Yano, Hiroshi Tazawa, Hiroyuki Kishimoto, Nobuhiro Narii, Hiroyuki Mizuguchi, Yasuo Urata, Shunsuke Kagawa, Toshiyoshi Fujiwara, Robert M. Hoffman

Research output: Contribution to journalArticle

Abstract

Melanoma is a highly recalcitrant cancer and transformative therapy is necessary for the cure of this disease. We recently developed a telomerase-dependent adenovirus containing the fluorescent protein Killer-Red. In the present report, we first determined the efficacy of Killer-Red adenovirus combined with laser irradiation on human melanoma cell lines in vitro. Cell viability of human melanoma cells was reduced in a dose-dependent and irradiation-time-dependent manner. We used an intradermal xenografted melanoma model in nude mice to determine efficacy of the Killer-Red adenovirus. Intratumoral injection of Killer-Red adenovirus, combined with laser irradiation, eradicated the melanoma indicating the potential of a new paradigm of cancer therapy.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalCell Cycle
DOIs
Publication statusAccepted/In press - Jul 26 2017

Fingerprint

Telomerase
Adenoviridae
Melanoma
Lasers
Nude Mice
Neoplasms
Cell Survival
In Vitro Techniques
Cell Line
Injections
Therapeutics

Keywords

  • adenovirus
  • killer-red
  • melanoma
  • nude mouse
  • photodynamic therapy
  • telomerase

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Eradication of melanoma in vitro and in vivo via targeting with a Killer-Red-containing telomerase-dependent adenovirus. / Takehara, Kiyoto; Yano, Shuuya; Tazawa, Hiroshi; Kishimoto, Hiroyuki; Narii, Nobuhiro; Mizuguchi, Hiroyuki; Urata, Yasuo; Kagawa, Shunsuke; Fujiwara, Toshiyoshi; Hoffman, Robert M.

In: Cell Cycle, 26.07.2017, p. 1-7.

Research output: Contribution to journalArticle

Takehara, Kiyoto ; Yano, Shuuya ; Tazawa, Hiroshi ; Kishimoto, Hiroyuki ; Narii, Nobuhiro ; Mizuguchi, Hiroyuki ; Urata, Yasuo ; Kagawa, Shunsuke ; Fujiwara, Toshiyoshi ; Hoffman, Robert M. / Eradication of melanoma in vitro and in vivo via targeting with a Killer-Red-containing telomerase-dependent adenovirus. In: Cell Cycle. 2017 ; pp. 1-7.
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