Equol and para-ethyl-phenol stimulate prostaglandin F2α secretion in bovine corpus luteum: Intracellular mechanisms of action

Izabela Woclawek-Potocka, Aleksandra Bober, Anna Korzekwa, Kiyoshi Okuda, Dariusz J. Skarzynski

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Corpus luteum (CL) is a reproductive gland that plays a crucial endocrine role in the regulation of the estrous cycle, fertility, and pregnancy in cattle. The main function of CL is secretion of progesterone (P4), an important hormone for establishment a successful pregnancy, whereas prostaglandin F (PGF), 17β-estradiol (E2) and testosterone (T) are implicated in the regulation of luteolysis. It has been shown that phytoestrogens may disrupt numerous reproductive functions on several levels of regulation and via different intracellular mechanisms. Using a cell-culture system of steroidogenic cells of the bovine CL, we determined effects of active phytoestrogen metabolites (equol and para-ethyl-phenol) on PGF, P4, and T synthesis in steroidogenic CL cells. Moreover, we examined the intracellular mechanisms of phytoestrogen metabolite actions. Phytoestrogen metabolites did not affect P4 production in steroidogenic CL cells. However, PGF and T were significantly stimulated by metabolites of phytoestrogens in the bovine steroidogenic CL cells. To study the intracellular mechanism of endogenous E2 and phytoestrogen metabolites action, steroidogenic cells were preincubated with a phospholipase C inhibitor (U73122), a protein kinase C inhibitor (staurosporine), an estrogen receptor antagonist (ICI) and a transcription inhibitor (actinomycin D) for 0.5 h, and then stimulated with para-ethyl-phenol, equol or E2. Only U73122 and staurosporine totally reduced the stimulatory effect of E2 on PGF production by the cells. ICI and actinomycin D only partially reduced E2 action on CL cells. In contrast, the stimulatory effect of phytoestrogen metabolites was totally inhibited by ICI and actinomycin D. Moreover, in contrast to E2 action, phytoestrogen metabolites did not cause intracellular calcium mobilization in the cells. The present study demonstrated that phytoestrogen metabolites stimulate PGF secretion in steroidogenic cells of the bovine CL via the estrogen receptor-dependent, genomic pathway.

Original languageEnglish
Pages (from-to)287-297
Number of pages11
JournalProstaglandins and Other Lipid Mediators
Volume79
Issue number3-4
DOIs
Publication statusPublished - May 2006

Fingerprint

Equol
Phytoestrogens
Dinoprost
Corpus Luteum
Phenol
Metabolites
Dactinomycin
Staurosporine
Luteolysis
Pregnancy
Estrous Cycle
Protein C Inhibitor
Type C Phospholipases
Transcription
Protein Kinase Inhibitors
Cell culture
Estrogen Receptors
Protein Kinase C
Progesterone
Fertility

Keywords

  • Cattle
  • Corpus luteum
  • PGF
  • Phytoestrogens

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Equol and para-ethyl-phenol stimulate prostaglandin F2α secretion in bovine corpus luteum : Intracellular mechanisms of action. / Woclawek-Potocka, Izabela; Bober, Aleksandra; Korzekwa, Anna; Okuda, Kiyoshi; Skarzynski, Dariusz J.

In: Prostaglandins and Other Lipid Mediators, Vol. 79, No. 3-4, 05.2006, p. 287-297.

Research output: Contribution to journalArticle

Woclawek-Potocka, Izabela ; Bober, Aleksandra ; Korzekwa, Anna ; Okuda, Kiyoshi ; Skarzynski, Dariusz J. / Equol and para-ethyl-phenol stimulate prostaglandin F2α secretion in bovine corpus luteum : Intracellular mechanisms of action. In: Prostaglandins and Other Lipid Mediators. 2006 ; Vol. 79, No. 3-4. pp. 287-297.
@article{fba22648ce514b818997e83d0938125e,
title = "Equol and para-ethyl-phenol stimulate prostaglandin F2α secretion in bovine corpus luteum: Intracellular mechanisms of action",
abstract = "Corpus luteum (CL) is a reproductive gland that plays a crucial endocrine role in the regulation of the estrous cycle, fertility, and pregnancy in cattle. The main function of CL is secretion of progesterone (P4), an important hormone for establishment a successful pregnancy, whereas prostaglandin F2α (PGF2α), 17β-estradiol (E2) and testosterone (T) are implicated in the regulation of luteolysis. It has been shown that phytoestrogens may disrupt numerous reproductive functions on several levels of regulation and via different intracellular mechanisms. Using a cell-culture system of steroidogenic cells of the bovine CL, we determined effects of active phytoestrogen metabolites (equol and para-ethyl-phenol) on PGF2α, P4, and T synthesis in steroidogenic CL cells. Moreover, we examined the intracellular mechanisms of phytoestrogen metabolite actions. Phytoestrogen metabolites did not affect P4 production in steroidogenic CL cells. However, PGF2α and T were significantly stimulated by metabolites of phytoestrogens in the bovine steroidogenic CL cells. To study the intracellular mechanism of endogenous E2 and phytoestrogen metabolites action, steroidogenic cells were preincubated with a phospholipase C inhibitor (U73122), a protein kinase C inhibitor (staurosporine), an estrogen receptor antagonist (ICI) and a transcription inhibitor (actinomycin D) for 0.5 h, and then stimulated with para-ethyl-phenol, equol or E2. Only U73122 and staurosporine totally reduced the stimulatory effect of E2 on PGF2α production by the cells. ICI and actinomycin D only partially reduced E2 action on CL cells. In contrast, the stimulatory effect of phytoestrogen metabolites was totally inhibited by ICI and actinomycin D. Moreover, in contrast to E2 action, phytoestrogen metabolites did not cause intracellular calcium mobilization in the cells. The present study demonstrated that phytoestrogen metabolites stimulate PGF2α secretion in steroidogenic cells of the bovine CL via the estrogen receptor-dependent, genomic pathway.",
keywords = "Cattle, Corpus luteum, PGF, Phytoestrogens",
author = "Izabela Woclawek-Potocka and Aleksandra Bober and Anna Korzekwa and Kiyoshi Okuda and Skarzynski, {Dariusz J.}",
year = "2006",
month = "5",
doi = "10.1016/j.prostaglandins.2006.03.003",
language = "English",
volume = "79",
pages = "287--297",
journal = "Prostaglandins and Other Lipid Mediators",
issn = "1098-8823",
publisher = "Elsevier Inc.",
number = "3-4",

}

TY - JOUR

T1 - Equol and para-ethyl-phenol stimulate prostaglandin F2α secretion in bovine corpus luteum

T2 - Intracellular mechanisms of action

AU - Woclawek-Potocka, Izabela

AU - Bober, Aleksandra

AU - Korzekwa, Anna

AU - Okuda, Kiyoshi

AU - Skarzynski, Dariusz J.

PY - 2006/5

Y1 - 2006/5

N2 - Corpus luteum (CL) is a reproductive gland that plays a crucial endocrine role in the regulation of the estrous cycle, fertility, and pregnancy in cattle. The main function of CL is secretion of progesterone (P4), an important hormone for establishment a successful pregnancy, whereas prostaglandin F2α (PGF2α), 17β-estradiol (E2) and testosterone (T) are implicated in the regulation of luteolysis. It has been shown that phytoestrogens may disrupt numerous reproductive functions on several levels of regulation and via different intracellular mechanisms. Using a cell-culture system of steroidogenic cells of the bovine CL, we determined effects of active phytoestrogen metabolites (equol and para-ethyl-phenol) on PGF2α, P4, and T synthesis in steroidogenic CL cells. Moreover, we examined the intracellular mechanisms of phytoestrogen metabolite actions. Phytoestrogen metabolites did not affect P4 production in steroidogenic CL cells. However, PGF2α and T were significantly stimulated by metabolites of phytoestrogens in the bovine steroidogenic CL cells. To study the intracellular mechanism of endogenous E2 and phytoestrogen metabolites action, steroidogenic cells were preincubated with a phospholipase C inhibitor (U73122), a protein kinase C inhibitor (staurosporine), an estrogen receptor antagonist (ICI) and a transcription inhibitor (actinomycin D) for 0.5 h, and then stimulated with para-ethyl-phenol, equol or E2. Only U73122 and staurosporine totally reduced the stimulatory effect of E2 on PGF2α production by the cells. ICI and actinomycin D only partially reduced E2 action on CL cells. In contrast, the stimulatory effect of phytoestrogen metabolites was totally inhibited by ICI and actinomycin D. Moreover, in contrast to E2 action, phytoestrogen metabolites did not cause intracellular calcium mobilization in the cells. The present study demonstrated that phytoestrogen metabolites stimulate PGF2α secretion in steroidogenic cells of the bovine CL via the estrogen receptor-dependent, genomic pathway.

AB - Corpus luteum (CL) is a reproductive gland that plays a crucial endocrine role in the regulation of the estrous cycle, fertility, and pregnancy in cattle. The main function of CL is secretion of progesterone (P4), an important hormone for establishment a successful pregnancy, whereas prostaglandin F2α (PGF2α), 17β-estradiol (E2) and testosterone (T) are implicated in the regulation of luteolysis. It has been shown that phytoestrogens may disrupt numerous reproductive functions on several levels of regulation and via different intracellular mechanisms. Using a cell-culture system of steroidogenic cells of the bovine CL, we determined effects of active phytoestrogen metabolites (equol and para-ethyl-phenol) on PGF2α, P4, and T synthesis in steroidogenic CL cells. Moreover, we examined the intracellular mechanisms of phytoestrogen metabolite actions. Phytoestrogen metabolites did not affect P4 production in steroidogenic CL cells. However, PGF2α and T were significantly stimulated by metabolites of phytoestrogens in the bovine steroidogenic CL cells. To study the intracellular mechanism of endogenous E2 and phytoestrogen metabolites action, steroidogenic cells were preincubated with a phospholipase C inhibitor (U73122), a protein kinase C inhibitor (staurosporine), an estrogen receptor antagonist (ICI) and a transcription inhibitor (actinomycin D) for 0.5 h, and then stimulated with para-ethyl-phenol, equol or E2. Only U73122 and staurosporine totally reduced the stimulatory effect of E2 on PGF2α production by the cells. ICI and actinomycin D only partially reduced E2 action on CL cells. In contrast, the stimulatory effect of phytoestrogen metabolites was totally inhibited by ICI and actinomycin D. Moreover, in contrast to E2 action, phytoestrogen metabolites did not cause intracellular calcium mobilization in the cells. The present study demonstrated that phytoestrogen metabolites stimulate PGF2α secretion in steroidogenic cells of the bovine CL via the estrogen receptor-dependent, genomic pathway.

KW - Cattle

KW - Corpus luteum

KW - PGF

KW - Phytoestrogens

UR - http://www.scopus.com/inward/record.url?scp=33746430151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746430151&partnerID=8YFLogxK

U2 - 10.1016/j.prostaglandins.2006.03.003

DO - 10.1016/j.prostaglandins.2006.03.003

M3 - Article

C2 - 16647642

AN - SCOPUS:33746430151

VL - 79

SP - 287

EP - 297

JO - Prostaglandins and Other Lipid Mediators

JF - Prostaglandins and Other Lipid Mediators

SN - 1098-8823

IS - 3-4

ER -