Epstein-Barr virus infection in the neoplastic and nonneoplastic cells of lymphoid malignancies

Norihiro Teramoto, Ashit Baran Sarker, Yuji Tonoyama, Tadashi Yoshino, Kazuhiko Hayashi, Kiyoshi Takahashi, Tadaatsu Akagi

Research output: Contribution to journalArticle

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Abstract

BACKGROUND. The Epstein-Barr virus (EBV) has been frequently detected in lymphoid malignancies. However, EBV infection in the nonneoplastic cells of lymphoid malignancies has not been extensively studied. METHODS. Four hundred nine cases of lymphoid malignancies including 377 non-Hodgkin's lymphoma (NHL) and 32 Hodgkin's disease (HD) were examined for EBV infection by EBER- 1 in situ hybridization (EBER-ISH), immunostaining against LMP-1, Epstein- Barr nuclear antigen 2 (EBNA2) and ZEBRA, and Southern hybridization using a BamHIW fragment as a probe. Double staining with EBER-ISH and immunostaining against CD20, CD45RO, and LMP-1 was performed in selected cases. RESULTS. Although EBER-1-positive cells (EPCs) were detected in 49 of 276 B-cell lymphomas, 31 of 100 T-cell lymphomas, 1 of 1 natural killer-cell lymphoma, and 17 of 32 HDs, almost all of the tumor cells were exclusively EBER-1- positive in the 10 NHL cases. Some EPCs were of different cell lineages than the tumor cells in 15 of the 26 NHLs examined by double staining. LMP-1, EBNA2, and ZEBRA were detected in 22, 4, and 3 cases, respectively. In 4 LMP- 1-positive HDs, double staining revealed that some EBER-1-positive Reed- Sternberg cells were negative for LMP-1. EBV genomic DNA was detected in 8 of the 39 examined cases. CONCLUSIONS. T-cell lymphomas contained EPCs more frequently than B cell lymphomas. Nonneoplastic lymphocytes were infected with EBV more frequently than lymphoma cells. Rowe's latency II may be unstable in lymphoid malignancies. Some NHLs, especially T-cell lymphoma, may provide favorable conditions for EBV infection of nonneoplastic lymphocytes.

Original languageEnglish
Pages (from-to)2339-2347
Number of pages9
JournalCancer
Volume77
Issue number11
DOIs
Publication statusPublished - Jun 1 1996

Fingerprint

Epstein-Barr Virus Infections
Lymphocytes
T-Cell Lymphoma
Human Herpesvirus 4
Neoplasms
Nuclear Antigens
B-Cell Lymphoma
Staining and Labeling
Non-Hodgkin's Lymphoma
In Situ Hybridization
Lymphoma
Reed-Sternberg Cells
Cell Lineage
Epstein-Barr virus encoded RNA 1
Hodgkin Disease
Natural Killer Cells
DNA

Keywords

  • EBER-1
  • EBNA2
  • Hodgkin's disease
  • in situ hybridization
  • LMP- 1
  • non-Hodgkin's malignant lymphoma
  • Southern hybridization
  • ZEBRA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Epstein-Barr virus infection in the neoplastic and nonneoplastic cells of lymphoid malignancies. / Teramoto, Norihiro; Sarker, Ashit Baran; Tonoyama, Yuji; Yoshino, Tadashi; Hayashi, Kazuhiko; Takahashi, Kiyoshi; Akagi, Tadaatsu.

In: Cancer, Vol. 77, No. 11, 01.06.1996, p. 2339-2347.

Research output: Contribution to journalArticle

Teramoto, Norihiro ; Sarker, Ashit Baran ; Tonoyama, Yuji ; Yoshino, Tadashi ; Hayashi, Kazuhiko ; Takahashi, Kiyoshi ; Akagi, Tadaatsu. / Epstein-Barr virus infection in the neoplastic and nonneoplastic cells of lymphoid malignancies. In: Cancer. 1996 ; Vol. 77, No. 11. pp. 2339-2347.
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abstract = "BACKGROUND. The Epstein-Barr virus (EBV) has been frequently detected in lymphoid malignancies. However, EBV infection in the nonneoplastic cells of lymphoid malignancies has not been extensively studied. METHODS. Four hundred nine cases of lymphoid malignancies including 377 non-Hodgkin's lymphoma (NHL) and 32 Hodgkin's disease (HD) were examined for EBV infection by EBER- 1 in situ hybridization (EBER-ISH), immunostaining against LMP-1, Epstein- Barr nuclear antigen 2 (EBNA2) and ZEBRA, and Southern hybridization using a BamHIW fragment as a probe. Double staining with EBER-ISH and immunostaining against CD20, CD45RO, and LMP-1 was performed in selected cases. RESULTS. Although EBER-1-positive cells (EPCs) were detected in 49 of 276 B-cell lymphomas, 31 of 100 T-cell lymphomas, 1 of 1 natural killer-cell lymphoma, and 17 of 32 HDs, almost all of the tumor cells were exclusively EBER-1- positive in the 10 NHL cases. Some EPCs were of different cell lineages than the tumor cells in 15 of the 26 NHLs examined by double staining. LMP-1, EBNA2, and ZEBRA were detected in 22, 4, and 3 cases, respectively. In 4 LMP- 1-positive HDs, double staining revealed that some EBER-1-positive Reed- Sternberg cells were negative for LMP-1. EBV genomic DNA was detected in 8 of the 39 examined cases. CONCLUSIONS. T-cell lymphomas contained EPCs more frequently than B cell lymphomas. Nonneoplastic lymphocytes were infected with EBV more frequently than lymphoma cells. Rowe's latency II may be unstable in lymphoid malignancies. Some NHLs, especially T-cell lymphoma, may provide favorable conditions for EBV infection of nonneoplastic lymphocytes.",
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AU - Hayashi, Kazuhiko

AU - Takahashi, Kiyoshi

AU - Akagi, Tadaatsu

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N2 - BACKGROUND. The Epstein-Barr virus (EBV) has been frequently detected in lymphoid malignancies. However, EBV infection in the nonneoplastic cells of lymphoid malignancies has not been extensively studied. METHODS. Four hundred nine cases of lymphoid malignancies including 377 non-Hodgkin's lymphoma (NHL) and 32 Hodgkin's disease (HD) were examined for EBV infection by EBER- 1 in situ hybridization (EBER-ISH), immunostaining against LMP-1, Epstein- Barr nuclear antigen 2 (EBNA2) and ZEBRA, and Southern hybridization using a BamHIW fragment as a probe. Double staining with EBER-ISH and immunostaining against CD20, CD45RO, and LMP-1 was performed in selected cases. RESULTS. Although EBER-1-positive cells (EPCs) were detected in 49 of 276 B-cell lymphomas, 31 of 100 T-cell lymphomas, 1 of 1 natural killer-cell lymphoma, and 17 of 32 HDs, almost all of the tumor cells were exclusively EBER-1- positive in the 10 NHL cases. Some EPCs were of different cell lineages than the tumor cells in 15 of the 26 NHLs examined by double staining. LMP-1, EBNA2, and ZEBRA were detected in 22, 4, and 3 cases, respectively. In 4 LMP- 1-positive HDs, double staining revealed that some EBER-1-positive Reed- Sternberg cells were negative for LMP-1. EBV genomic DNA was detected in 8 of the 39 examined cases. CONCLUSIONS. T-cell lymphomas contained EPCs more frequently than B cell lymphomas. Nonneoplastic lymphocytes were infected with EBV more frequently than lymphoma cells. Rowe's latency II may be unstable in lymphoid malignancies. Some NHLs, especially T-cell lymphoma, may provide favorable conditions for EBV infection of nonneoplastic lymphocytes.

AB - BACKGROUND. The Epstein-Barr virus (EBV) has been frequently detected in lymphoid malignancies. However, EBV infection in the nonneoplastic cells of lymphoid malignancies has not been extensively studied. METHODS. Four hundred nine cases of lymphoid malignancies including 377 non-Hodgkin's lymphoma (NHL) and 32 Hodgkin's disease (HD) were examined for EBV infection by EBER- 1 in situ hybridization (EBER-ISH), immunostaining against LMP-1, Epstein- Barr nuclear antigen 2 (EBNA2) and ZEBRA, and Southern hybridization using a BamHIW fragment as a probe. Double staining with EBER-ISH and immunostaining against CD20, CD45RO, and LMP-1 was performed in selected cases. RESULTS. Although EBER-1-positive cells (EPCs) were detected in 49 of 276 B-cell lymphomas, 31 of 100 T-cell lymphomas, 1 of 1 natural killer-cell lymphoma, and 17 of 32 HDs, almost all of the tumor cells were exclusively EBER-1- positive in the 10 NHL cases. Some EPCs were of different cell lineages than the tumor cells in 15 of the 26 NHLs examined by double staining. LMP-1, EBNA2, and ZEBRA were detected in 22, 4, and 3 cases, respectively. In 4 LMP- 1-positive HDs, double staining revealed that some EBER-1-positive Reed- Sternberg cells were negative for LMP-1. EBV genomic DNA was detected in 8 of the 39 examined cases. CONCLUSIONS. T-cell lymphomas contained EPCs more frequently than B cell lymphomas. Nonneoplastic lymphocytes were infected with EBV more frequently than lymphoma cells. Rowe's latency II may be unstable in lymphoid malignancies. Some NHLs, especially T-cell lymphoma, may provide favorable conditions for EBV infection of nonneoplastic lymphocytes.

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