EPR study of Cu(II) in N-terminal lobe fragment of hen ovotransferrin

Teruyuki Kawabata, Masayoshi Fujisawa, Akira I. Hida, Shigeru Okada

Research output: Contribution to journalArticle

Abstract

The N-terminal lobe fragment of hen ovotransferrin was prepared by trypsin digestion and the following gel filtration and ion-exchange chromatography. EPR spectra of Cu(II) complex of the N-terminal fragment were recorded independently from the C-terminal lobe and a major spectrum of the copper(II) complex showed g = 2.300 and g = 2.061 with ACu = 14.0 mT, ACu = 2.20 mT, which had a superhyperfine splitting (AN = 1.44 mT) of a nitrogen ligand, which may correspond to type A-1 site of copper binding position in the N-terminal lobe of hen ovotransferrin.

Original languageEnglish
Pages (from-to)858-859
Number of pages2
JournalChemistry Letters
Issue number9
Publication statusPublished - 2001

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Conalbumin
Paramagnetic resonance
Copper
Chromatography
Trypsin
Ion exchange
Nitrogen
Gels
Ligands

ASJC Scopus subject areas

  • Chemistry(all)

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EPR study of Cu(II) in N-terminal lobe fragment of hen ovotransferrin. / Kawabata, Teruyuki; Fujisawa, Masayoshi; Hida, Akira I.; Okada, Shigeru.

In: Chemistry Letters, No. 9, 2001, p. 858-859.

Research output: Contribution to journalArticle

Kawabata, Teruyuki ; Fujisawa, Masayoshi ; Hida, Akira I. ; Okada, Shigeru. / EPR study of Cu(II) in N-terminal lobe fragment of hen ovotransferrin. In: Chemistry Letters. 2001 ; No. 9. pp. 858-859.
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