Epitopes on β2-GPI recognized by anticardiolipin antibodies

T. Koike, K. Ichikawa, H. Kasahara, T. Atsumi, A. Tsutsumi, Eiji Matsuura

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Anticardiolipin antibodies (aCL) found in sera from patients with antiphospholipid syndrome recognize a cryptic epitope that appears on the β2-glycoprotein I (β2-GPI) molecule when β2-GPI interacts with a lipid membrane composed of negatively charged phospholipid or when β2-GPI is adsorbed on a polyoxygenated polystyrene plate. A homology based model of β2-GPI was constructed based on the NMR coordinates of sushi domains of human factor H. The conformation was like a cylinder consisting of five domains, its IV and V domains being glued by electrostatic interaction. We used phage-displayed random peptide libraries to search the epitopes of human aCL. Structures similar to consensus sequences selected by a biopanning method was found on domain IV of β2-GPI.

Original languageEnglish
JournalLupus
Volume7
Issue numberSUPPL. 2
Publication statusPublished - 1998

Fingerprint

Anticardiolipin Antibodies
Epitopes
Glycoproteins
Peptide Library
Antiphospholipid Syndrome
Polystyrenes
Consensus Sequence
Membrane Lipids
Static Electricity
Bacteriophages
Phospholipids
Serum

Keywords

  • β-glycoprotein I
  • Anticardiolipin antibodies
  • Epitopes
  • Structure of β-GPI

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Koike, T., Ichikawa, K., Kasahara, H., Atsumi, T., Tsutsumi, A., & Matsuura, E. (1998). Epitopes on β2-GPI recognized by anticardiolipin antibodies. Lupus, 7(SUPPL. 2).

Epitopes on β2-GPI recognized by anticardiolipin antibodies. / Koike, T.; Ichikawa, K.; Kasahara, H.; Atsumi, T.; Tsutsumi, A.; Matsuura, Eiji.

In: Lupus, Vol. 7, No. SUPPL. 2, 1998.

Research output: Contribution to journalArticle

Koike, T, Ichikawa, K, Kasahara, H, Atsumi, T, Tsutsumi, A & Matsuura, E 1998, 'Epitopes on β2-GPI recognized by anticardiolipin antibodies', Lupus, vol. 7, no. SUPPL. 2.
Koike T, Ichikawa K, Kasahara H, Atsumi T, Tsutsumi A, Matsuura E. Epitopes on β2-GPI recognized by anticardiolipin antibodies. Lupus. 1998;7(SUPPL. 2).
Koike, T. ; Ichikawa, K. ; Kasahara, H. ; Atsumi, T. ; Tsutsumi, A. ; Matsuura, Eiji. / Epitopes on β2-GPI recognized by anticardiolipin antibodies. In: Lupus. 1998 ; Vol. 7, No. SUPPL. 2.
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