Epitope-defined monoclonal antibodies against multiplexin collagens demonstrate that type XV and XVIII collagens are expressed in specialized basement membranes

Yasuko Tomono, Ichiro Naito, Kaori Ando, Tomoko Yonezawa, Yoshikazu Sado, Satoshi Hirakawa, Jirô Arata, Tohru Okigaki, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Type XV and type XVIII collagens are classified as part of multiplexin collagen superfamily and their C-terminal parts, endostatin and restin, respectively, have been shown to be anti-angiogenic in vivo and in vitro. The α1(XV) and α1(XVIII) collagen chains are reported to be localized mainly in the basement membrane zone, but their distributions in blood vessels and nonvascular tissues have yet to be thoroughly clarified. In the present study, we raised monoclonal antibodies against synthetic peptides of human α1(XV) and α1(XVIII) chains and used them for extensive investigation of the distribution of these chains. We came to the conclusion that nonvascular BMs contain mainly one of two types: subepithelial basement membranes that contained type XVIII in general, or skeletal and cardiac muscles that harbored mainly type XV. But basement membranes surrounding smooth muscle cells in vascular tissues contained one or both of them, depending on their locations. Interestingly, continuous capillaries contained both type XV and type XVIII collagens in their basement membranes; however, fenestrated or specialized capillaries such as glomeruli, liver sinusoids, lung alveoli, and splenic sinusoids expressed only type XVIII in their basement membranes, lacking type XV. This observation could imply that different functions of basement membranes in various tissues and organs use different mechanisms for the endogenous control of angiogenesis.

Original languageEnglish
Pages (from-to)9-20
Number of pages12
JournalCell Structure and Function
Volume27
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Collagen Type XVIII
Basement Membrane
Epitopes
Collagen
Monoclonal Antibodies
Blood Vessels
Endostatins
Smooth Muscle Myocytes
Myocardium
Skeletal Muscle
Lung
Peptides
Liver

Keywords

  • Basement membrane
  • Capillary
  • Smooth muscle cell
  • Type XV collagen
  • Type XVIII collagen

ASJC Scopus subject areas

  • Physiology
  • Structural Biology
  • Cell Biology

Cite this

Epitope-defined monoclonal antibodies against multiplexin collagens demonstrate that type XV and XVIII collagens are expressed in specialized basement membranes. / Tomono, Yasuko; Naito, Ichiro; Ando, Kaori; Yonezawa, Tomoko; Sado, Yoshikazu; Hirakawa, Satoshi; Arata, Jirô; Okigaki, Tohru; Ninomiya, Yoshifumi.

In: Cell Structure and Function, Vol. 27, No. 1, 2002, p. 9-20.

Research output: Contribution to journalArticle

Tomono, Yasuko ; Naito, Ichiro ; Ando, Kaori ; Yonezawa, Tomoko ; Sado, Yoshikazu ; Hirakawa, Satoshi ; Arata, Jirô ; Okigaki, Tohru ; Ninomiya, Yoshifumi. / Epitope-defined monoclonal antibodies against multiplexin collagens demonstrate that type XV and XVIII collagens are expressed in specialized basement membranes. In: Cell Structure and Function. 2002 ; Vol. 27, No. 1. pp. 9-20.
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