Epitope analysis of HLA-DR-restricted helper T-cell responses to der p II, a major allergen molecule of dermatophagoides pteronyssinus

M. Okano, T. Nagano, M. Nakada, Y. Masuda, K. Kino, H. Yasueda, Y. Nose, Y. Nishimura, N. Ohta

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

T-cell epitopes of Der p II, a major allergen of Dermatophagoides pteronyssinus, were analyzed by using human T-cell clones. We tested 38 cloned T cells from two Japanese patients with allergic rhinitis, and identified at least two peptides (K33-T47 and I58-C73) as helper T-cell epitopes. The former epitope was shown to be restricted by HLA-DRB1*1502, and the latter by HLA-DRB1*0405, both of which are typical Japanese HLA-DR alleles, suggesting that those T-cell epitopes might be important for the onset of house-dust mite allergy in the Japanese population. We prepared 15 analog peptides of the HLA-DRB1*1 502-restricted 15-mer peptide. Of those 15 residues, five (F35, L37, A39, F41, and E42) were critical for the epitope activity, and three residues (F35, A39, and E42) seemed to be included in anchor motifs for HLA-DRB1*1502. The epitope peptide was also recognized by HLA-DRB1*1502-positive healthy donors; however, only allergic T cells showed Th2 functions. Antigen-presenting cells of nonallergic donors were able to activate allergic T cells to express Th2 function. This seemed to suggest that antigen recognition of T cells, as well as additional unknown factors which promote Th2, rather than Th1, responses, might be important for the onset of house-dust mite allergy.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume51
Issue number1
Publication statusPublished - 1996

Fingerprint

Dermatophagoides Antigens
HLA-DR Antigens
HLA-DRB1 Chains
Helper-Inducer T-Lymphocytes
Epitopes
T-Lymphocyte Epitopes
T-Lymphocytes
Pyroglyphidae
Peptides
Hypersensitivity
Tissue Donors
Antigen-Presenting Cells
Clone Cells
Alleles
Dermatophagoides pteronyssinus antigen p 2
Antigens
Population

Keywords

  • Der p II
  • HLA-DR
  • House-dust-mite allergy
  • Synthetic peptides
  • T-cell epitope
  • Th2 cell

ASJC Scopus subject areas

  • Immunology

Cite this

Epitope analysis of HLA-DR-restricted helper T-cell responses to der p II, a major allergen molecule of dermatophagoides pteronyssinus. / Okano, M.; Nagano, T.; Nakada, M.; Masuda, Y.; Kino, K.; Yasueda, H.; Nose, Y.; Nishimura, Y.; Ohta, N.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 51, No. 1, 1996, p. 29-35.

Research output: Contribution to journalArticle

Okano, M, Nagano, T, Nakada, M, Masuda, Y, Kino, K, Yasueda, H, Nose, Y, Nishimura, Y & Ohta, N 1996, 'Epitope analysis of HLA-DR-restricted helper T-cell responses to der p II, a major allergen molecule of dermatophagoides pteronyssinus', Allergy: European Journal of Allergy and Clinical Immunology, vol. 51, no. 1, pp. 29-35.
Okano, M. ; Nagano, T. ; Nakada, M. ; Masuda, Y. ; Kino, K. ; Yasueda, H. ; Nose, Y. ; Nishimura, Y. ; Ohta, N. / Epitope analysis of HLA-DR-restricted helper T-cell responses to der p II, a major allergen molecule of dermatophagoides pteronyssinus. In: Allergy: European Journal of Allergy and Clinical Immunology. 1996 ; Vol. 51, No. 1. pp. 29-35.
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