Epigenomic modification in rice controls meiotic recombination and segregation distortion

Yoshiki Habu, Tsuyu Ando, Sachie Ito, Kiyotaka Nagaki, Naoki Kishimoto, Fumio Taguchi-Shiobara, Hisataka Numa, Katsushi Yamaguchi, Shuji Shigenobu, Minoru Murata, Tetsuo Meshi, Masahiro Yano

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The low frequency of meiotic recombination in chromosomal regions other than hotspots is a general obstacle to efficient breeding. A number of active genes are present in recombination-repressed centromeric regions in higher eukaryotes, suggesting that suppression of meiotic recombination prevents shuffling of genes within a centromeric region. In this study, by using an inter-subspecific cross of Oryza sativa L., we show that modification of inactive chromatin states by either genetic or chemical inhibition of chromatin modifying proteins induced changes in both the position of meiotic recombination and, unexpectedly, the pattern of segregation distortion of parental alleles. Antisense knockdown of rice homologues of DECREASE IN DNA METHYLATION1, which is required for the maintenance of heterochromatin in Arabidopsis thaliana, induced a recombination hotspot in a centromeric region accompanied by a steep increase in the proportion of heterozygotes. Our results describe a previously undocumented phenomenon in which artificial chromatin modification could be used to change the pattern of segregation distortion in rice and open up novel possibilities for efficient crop breeding.

Original languageEnglish
JournalMolecular Breeding
Volume35
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

Fingerprint

segregation distortion
Epigenomics
epigenetics
Genetic Recombination
chromatin
rice
Chromatin
Breeding
heterochromatin
plant breeding
eukaryotic cells
heterozygosity
Oryza sativa
genes
Arabidopsis thaliana
Heterochromatin
alleles
Heterozygote
Eukaryota
Arabidopsis

Keywords

  • DECREASE IN DNA METHYLATION1
  • Meiotic recombination
  • Rice
  • Segregation distortion

ASJC Scopus subject areas

  • Plant Science
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Biotechnology

Cite this

Epigenomic modification in rice controls meiotic recombination and segregation distortion. / Habu, Yoshiki; Ando, Tsuyu; Ito, Sachie; Nagaki, Kiyotaka; Kishimoto, Naoki; Taguchi-Shiobara, Fumio; Numa, Hisataka; Yamaguchi, Katsushi; Shigenobu, Shuji; Murata, Minoru; Meshi, Tetsuo; Yano, Masahiro.

In: Molecular Breeding, Vol. 35, No. 4, 01.04.2015.

Research output: Contribution to journalArticle

Habu, Y, Ando, T, Ito, S, Nagaki, K, Kishimoto, N, Taguchi-Shiobara, F, Numa, H, Yamaguchi, K, Shigenobu, S, Murata, M, Meshi, T & Yano, M 2015, 'Epigenomic modification in rice controls meiotic recombination and segregation distortion', Molecular Breeding, vol. 35, no. 4. https://doi.org/10.1007/s11032-015-0299-0
Habu, Yoshiki ; Ando, Tsuyu ; Ito, Sachie ; Nagaki, Kiyotaka ; Kishimoto, Naoki ; Taguchi-Shiobara, Fumio ; Numa, Hisataka ; Yamaguchi, Katsushi ; Shigenobu, Shuji ; Murata, Minoru ; Meshi, Tetsuo ; Yano, Masahiro. / Epigenomic modification in rice controls meiotic recombination and segregation distortion. In: Molecular Breeding. 2015 ; Vol. 35, No. 4.
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