Epigenome confrontation triggers immediate reprogramming of DNA methylation and transposon silencing in Arabidopsis thaliana F1 epihybrids

Mélanie Rigal, Claude Becker, Thierry Pélissier, Romain Pogorelcnik, Jane Devos, Yoko Ikeda, Detlef Weigel, Olivier Mathieu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Genes and transposons can exist in variable DNA methylation states, with potentially differential transcription. How these epialleles emerge is poorly understood. Here, we show that crossing an Arabidopsis thaliana plant with a hypomethylated genome and a normally methylated WT individual results, already in the F1 generation, in widespread changes in DNA methylation and transcription patterns. Novel nonparental and heritable epialleles arise at many genic loci, including a locus that itself controls DNA methylation patterns, but with most of the changes affecting pericentromeric transposons. Although a subset of transposons show immediate resilencing, a large number display decreased DNA methylation, which is associated with de novo or enhanced transcriptional activation and can translate into transposon mobilization in the progeny. Our findings reveal that the combination of distinct epigenomes can be viewed as an epigenomic shock, which is characterized by a round of epigenetic variation creating novel patterns of gene and TE regulation.

Original languageEnglish
Pages (from-to)E2083-E2092
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number14
DOIs
Publication statusPublished - Apr 5 2016

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DNA Transposable Elements
DNA Methylation
Arabidopsis
Epigenomics
Internal-External Control
Transcriptional Activation
Genes
Shock
Genome

Keywords

  • Arabidopsis
  • DNA methylation
  • Gene silencing
  • Transcription
  • Transposable elements

ASJC Scopus subject areas

  • General

Cite this

Epigenome confrontation triggers immediate reprogramming of DNA methylation and transposon silencing in Arabidopsis thaliana F1 epihybrids. / Rigal, Mélanie; Becker, Claude; Pélissier, Thierry; Pogorelcnik, Romain; Devos, Jane; Ikeda, Yoko; Weigel, Detlef; Mathieu, Olivier.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 14, 05.04.2016, p. E2083-E2092.

Research output: Contribution to journalArticle

Rigal, Mélanie ; Becker, Claude ; Pélissier, Thierry ; Pogorelcnik, Romain ; Devos, Jane ; Ikeda, Yoko ; Weigel, Detlef ; Mathieu, Olivier. / Epigenome confrontation triggers immediate reprogramming of DNA methylation and transposon silencing in Arabidopsis thaliana F1 epihybrids. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 14. pp. E2083-E2092.
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