Epigenetic regulation of the sex determination gene megi in polyploid persimmon

Takashi Akagi, Isabelle M. Henry, Takashi Kawai, Luca Comai, Ryutaro Tao

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Epigenetic regulation can add a flexible layer to genetic variation, potentially enabling long-term but reversible cis-regulatory changes to an allele while maintaining its DNA sequence. Here, we present a case in which alternative epigenetic states lead toreversible sex determination in the hexaploid persimmon Diospyros kaki. Previously, we elucidated the molecular mechanismof sexdetermination in diploid persimmon and demonstrated the action of a Y-encoded sex determinant pseudogene called OGI, whichproduces small RNAs targeting the autosomal gene MeGI, resulting in separate male and female individuals (dioecy). We contrastthese findings with the discovery, in hexaploid persimmon, of an additional layer of regulation in the form of DNA methylation of theMeGI promoter associated with the production of both male and female flowers in genetically male trees. Consistent with this model, developing male buds exhibited higher methylation levels across the MeGI promoter than developing female flowers from either monoecious or female trees. Additionally, a DNA methylation inhibitor induced developing male buds to form feminized flowers. Concurrently, in Y-chromosome-carrying trees, the expression of OGI is silenced by the presence of a SINE (short interspersed nuclear element)-like insertion in the OGI promoter. Our findings provide an example of an adaptive scenario in volving epigenetic plasticity.

Original languageEnglish
Pages (from-to)2905-2915
Number of pages11
JournalPlant Cell
Volume28
Issue number12
DOIs
Publication statusPublished - Dec 1 2016
Externally publishedYes

Fingerprint

Diospyros
Polyploidy
persimmons
Epigenomics
epigenetics
polyploidy
female flowers
promoter regions
DNA methylation
hexaploidy
gender
buds
Genes
dioecy
Diospyros kaki
DNA Methylation
male flowers
genes
pseudogenes
Y chromosome

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Epigenetic regulation of the sex determination gene megi in polyploid persimmon. / Akagi, Takashi; Henry, Isabelle M.; Kawai, Takashi; Comai, Luca; Tao, Ryutaro.

In: Plant Cell, Vol. 28, No. 12, 01.12.2016, p. 2905-2915.

Research output: Contribution to journalArticle

Akagi, Takashi ; Henry, Isabelle M. ; Kawai, Takashi ; Comai, Luca ; Tao, Ryutaro. / Epigenetic regulation of the sex determination gene megi in polyploid persimmon. In: Plant Cell. 2016 ; Vol. 28, No. 12. pp. 2905-2915.
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