Epidermal growth factor stimulates proliferation of mouse uterine epithelial cells in primary culture

Masahiro Shiraga, Noriko Komatsu, Kiyoshi Teshigawara, Akinobu Okada, Sakae Takeuchi, Hiroshi Fukamachi, Sumio Takahashi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) is one of growth factors that are thought to mediate the stimulatory effects of estrogen on the proliferation of uterine epithelial cells. The present study was attempted to obtain direct evidence for the mitogenic effects of EGF on uterine epithelial cells, and to prove that EGF and EGF receptors are expressed in these cells. Mouse uterine epithelial cells were isolated from immature female mice and cultured with or without EGF for 5 days. EGF (1 to 100 ng/ml) significantly increased the number of uterine epithelial cells, and the maximal growth (141.9±8.3% of controls) was obtained at a dose of 10 ng/ml. In addition, EGF (0.1 to 100 ng/ml) increased the number of DNA-synthesizing cells immunocytochemically detected by bromodeoxyuridine uptake to the nucleus. Northern blot analysis revealed that the uterine epithelial cells expressed both EGF mRNA (4.7 kb) and EGF receptor mRNAs (10.5, 6.6, and 2.7 kb) These results suggest that the proliferation of uterine epithelial cells is regulated by the paracrine and/ or autocrine action of EGF. Our previous study demonstrated the mitogenic effect of IGF-I on uterine epithelial cells. To examine whether the EGF-and IGF-I signaling act at the same level in the regulation of the proliferation of uterine epithelial cells, the cultured cells were simultaneously treated with IGF-I and EGF. IGF-I was found to additively stimulate the mitogenic effects of EGF, suggesting that the EGF-induced growth of uterine epithelial cells is distinct from IGF-I-induced growth.

Original languageEnglish
Pages (from-to)661-666
Number of pages6
JournalZoological Science
Volume17
Issue number5
Publication statusPublished - Jul 2000

Fingerprint

epidermal growth factor
epithelial cells
mice
insulin-like growth factor I
Northern blotting
cultured cells
growth factors
estrogens
immatures
cells

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Epidermal growth factor stimulates proliferation of mouse uterine epithelial cells in primary culture. / Shiraga, Masahiro; Komatsu, Noriko; Teshigawara, Kiyoshi; Okada, Akinobu; Takeuchi, Sakae; Fukamachi, Hiroshi; Takahashi, Sumio.

In: Zoological Science, Vol. 17, No. 5, 07.2000, p. 661-666.

Research output: Contribution to journalArticle

Shiraga, Masahiro ; Komatsu, Noriko ; Teshigawara, Kiyoshi ; Okada, Akinobu ; Takeuchi, Sakae ; Fukamachi, Hiroshi ; Takahashi, Sumio. / Epidermal growth factor stimulates proliferation of mouse uterine epithelial cells in primary culture. In: Zoological Science. 2000 ; Vol. 17, No. 5. pp. 661-666.
@article{431589cb10c9433692c3b014541e3087,
title = "Epidermal growth factor stimulates proliferation of mouse uterine epithelial cells in primary culture",
abstract = "Epidermal growth factor (EGF) is one of growth factors that are thought to mediate the stimulatory effects of estrogen on the proliferation of uterine epithelial cells. The present study was attempted to obtain direct evidence for the mitogenic effects of EGF on uterine epithelial cells, and to prove that EGF and EGF receptors are expressed in these cells. Mouse uterine epithelial cells were isolated from immature female mice and cultured with or without EGF for 5 days. EGF (1 to 100 ng/ml) significantly increased the number of uterine epithelial cells, and the maximal growth (141.9±8.3{\%} of controls) was obtained at a dose of 10 ng/ml. In addition, EGF (0.1 to 100 ng/ml) increased the number of DNA-synthesizing cells immunocytochemically detected by bromodeoxyuridine uptake to the nucleus. Northern blot analysis revealed that the uterine epithelial cells expressed both EGF mRNA (4.7 kb) and EGF receptor mRNAs (10.5, 6.6, and 2.7 kb) These results suggest that the proliferation of uterine epithelial cells is regulated by the paracrine and/ or autocrine action of EGF. Our previous study demonstrated the mitogenic effect of IGF-I on uterine epithelial cells. To examine whether the EGF-and IGF-I signaling act at the same level in the regulation of the proliferation of uterine epithelial cells, the cultured cells were simultaneously treated with IGF-I and EGF. IGF-I was found to additively stimulate the mitogenic effects of EGF, suggesting that the EGF-induced growth of uterine epithelial cells is distinct from IGF-I-induced growth.",
author = "Masahiro Shiraga and Noriko Komatsu and Kiyoshi Teshigawara and Akinobu Okada and Sakae Takeuchi and Hiroshi Fukamachi and Sumio Takahashi",
year = "2000",
month = "7",
language = "English",
volume = "17",
pages = "661--666",
journal = "Zoological Science",
issn = "0289-0003",
publisher = "Zoological Society of Japan",
number = "5",

}

TY - JOUR

T1 - Epidermal growth factor stimulates proliferation of mouse uterine epithelial cells in primary culture

AU - Shiraga, Masahiro

AU - Komatsu, Noriko

AU - Teshigawara, Kiyoshi

AU - Okada, Akinobu

AU - Takeuchi, Sakae

AU - Fukamachi, Hiroshi

AU - Takahashi, Sumio

PY - 2000/7

Y1 - 2000/7

N2 - Epidermal growth factor (EGF) is one of growth factors that are thought to mediate the stimulatory effects of estrogen on the proliferation of uterine epithelial cells. The present study was attempted to obtain direct evidence for the mitogenic effects of EGF on uterine epithelial cells, and to prove that EGF and EGF receptors are expressed in these cells. Mouse uterine epithelial cells were isolated from immature female mice and cultured with or without EGF for 5 days. EGF (1 to 100 ng/ml) significantly increased the number of uterine epithelial cells, and the maximal growth (141.9±8.3% of controls) was obtained at a dose of 10 ng/ml. In addition, EGF (0.1 to 100 ng/ml) increased the number of DNA-synthesizing cells immunocytochemically detected by bromodeoxyuridine uptake to the nucleus. Northern blot analysis revealed that the uterine epithelial cells expressed both EGF mRNA (4.7 kb) and EGF receptor mRNAs (10.5, 6.6, and 2.7 kb) These results suggest that the proliferation of uterine epithelial cells is regulated by the paracrine and/ or autocrine action of EGF. Our previous study demonstrated the mitogenic effect of IGF-I on uterine epithelial cells. To examine whether the EGF-and IGF-I signaling act at the same level in the regulation of the proliferation of uterine epithelial cells, the cultured cells were simultaneously treated with IGF-I and EGF. IGF-I was found to additively stimulate the mitogenic effects of EGF, suggesting that the EGF-induced growth of uterine epithelial cells is distinct from IGF-I-induced growth.

AB - Epidermal growth factor (EGF) is one of growth factors that are thought to mediate the stimulatory effects of estrogen on the proliferation of uterine epithelial cells. The present study was attempted to obtain direct evidence for the mitogenic effects of EGF on uterine epithelial cells, and to prove that EGF and EGF receptors are expressed in these cells. Mouse uterine epithelial cells were isolated from immature female mice and cultured with or without EGF for 5 days. EGF (1 to 100 ng/ml) significantly increased the number of uterine epithelial cells, and the maximal growth (141.9±8.3% of controls) was obtained at a dose of 10 ng/ml. In addition, EGF (0.1 to 100 ng/ml) increased the number of DNA-synthesizing cells immunocytochemically detected by bromodeoxyuridine uptake to the nucleus. Northern blot analysis revealed that the uterine epithelial cells expressed both EGF mRNA (4.7 kb) and EGF receptor mRNAs (10.5, 6.6, and 2.7 kb) These results suggest that the proliferation of uterine epithelial cells is regulated by the paracrine and/ or autocrine action of EGF. Our previous study demonstrated the mitogenic effect of IGF-I on uterine epithelial cells. To examine whether the EGF-and IGF-I signaling act at the same level in the regulation of the proliferation of uterine epithelial cells, the cultured cells were simultaneously treated with IGF-I and EGF. IGF-I was found to additively stimulate the mitogenic effects of EGF, suggesting that the EGF-induced growth of uterine epithelial cells is distinct from IGF-I-induced growth.

UR - http://www.scopus.com/inward/record.url?scp=0034336891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034336891&partnerID=8YFLogxK

M3 - Article

C2 - 18517302

AN - SCOPUS:0034336891

VL - 17

SP - 661

EP - 666

JO - Zoological Science

JF - Zoological Science

SN - 0289-0003

IS - 5

ER -