Epidermal growth factor receptor-dependent cytotoxicity for human squamous carcinoma cell lines of a conjugate composed of human EGF and RNase 1

Hiromitsu Jinno; Masakazu Ueda; Soji Ozawa; Tadashi Ikeda; Kohji Enomoto; Kyriakos Psarras; Masaki Kitajima; Hidenori Yamada; Masaharu Seno

doi:10.1016/0024-3205(96)00174-9

Epidermal growth factor receptor-dependent cytotoxicity for human squamous carcinoma cell lines of a conjugate composed of human EGF and RNase 1

Hiromitsu Jinno, Masakazu Ueda, Soji Ozawa, Tadashi Ikeda, Kohji Enomoto, Kyriakos Psarras, Masaki Kitajima, Hidenori Yamada, Masaharu Seno

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Recombinant human ribonuclease 1 (RNase 1) was chemically linked to recombinant human epidermal growth factor (EGF). The EGF-RNase conjugate showed dose-dependent cytotoxicity for EGF receptor-overexpressing A431 and TE-8 human squamous carcinoma cells with an IC₅₀ of 2 x 10^-7 M and 10^-6 M, respectively, whereas the IC₅₀ of RNase alone was almost 10^-4M. An unconjugated mixture of EGF and RNase had no greater effect than RNase alone. The conjugate showed no detectable cytotoxicity against EGF receptor-deficient small cell lung cancer cells (H69). Addition to excess EGF in the medium protected A431 cells from the EGF-RNase conjugate cytotoxicity. The cytotoxicity of the EGF-RNase conjugate was positively correlated with the EGF receptor numbers of each cell line. The chimeric toxin composed of only human proteins might be a more useful anti-cancer agent with less immunogenicity than the conventional chimeric toxins.

Original language	English
Pages (from-to)	1901-1908
Number of pages	8
Journal	Life Sciences
Volume	58
Issue number	21
DOIs	https://doi.org/10.1016/0024-3205(96)00174-9
Publication status	Published - Apr 19 1996

Keywords

conjugate
epidermal growth factor
ribonuclease
squamous carcinoma

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0024-3205(96)00174-9

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title = "Epidermal growth factor receptor-dependent cytotoxicity for human squamous carcinoma cell lines of a conjugate composed of human EGF and RNase 1",

abstract = "Recombinant human ribonuclease 1 (RNase 1) was chemically linked to recombinant human epidermal growth factor (EGF). The EGF-RNase conjugate showed dose-dependent cytotoxicity for EGF receptor-overexpressing A431 and TE-8 human squamous carcinoma cells with an IC50 of 2 x 10-7 M and 10-6 M, respectively, whereas the IC50 of RNase alone was almost 10-4M. An unconjugated mixture of EGF and RNase had no greater effect than RNase alone. The conjugate showed no detectable cytotoxicity against EGF receptor-deficient small cell lung cancer cells (H69). Addition to excess EGF in the medium protected A431 cells from the EGF-RNase conjugate cytotoxicity. The cytotoxicity of the EGF-RNase conjugate was positively correlated with the EGF receptor numbers of each cell line. The chimeric toxin composed of only human proteins might be a more useful anti-cancer agent with less immunogenicity than the conventional chimeric toxins.",

keywords = "conjugate, epidermal growth factor, ribonuclease, squamous carcinoma",

author = "Hiromitsu Jinno and Masakazu Ueda and Soji Ozawa and Tadashi Ikeda and Kohji Enomoto and Kyriakos Psarras and Masaki Kitajima and Hidenori Yamada and Masaharu Seno",

note = "Funding Information: We thankM s. Sachiko Matsudaa nd Ms. Yuki Inaba for theire xcellentt echnicala ssistancea nd Dr. Norifumi Hirota for valuablea dvice.T his work was supportedin part by a grant-in-aid from the Ministry of Education,C ulture, and Science,J apan.",

year = "1996",

month = apr,

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doi = "10.1016/0024-3205(96)00174-9",

language = "English",

volume = "58",

pages = "1901--1908",

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T1 - Epidermal growth factor receptor-dependent cytotoxicity for human squamous carcinoma cell lines of a conjugate composed of human EGF and RNase 1

AU - Jinno, Hiromitsu

AU - Ueda, Masakazu

AU - Ozawa, Soji

AU - Ikeda, Tadashi

AU - Enomoto, Kohji

AU - Psarras, Kyriakos

AU - Kitajima, Masaki

AU - Yamada, Hidenori

AU - Seno, Masaharu

N1 - Funding Information: We thankM s. Sachiko Matsudaa nd Ms. Yuki Inaba for theire xcellentt echnicala ssistancea nd Dr. Norifumi Hirota for valuablea dvice.T his work was supportedin part by a grant-in-aid from the Ministry of Education,C ulture, and Science,J apan.

PY - 1996/4/19

Y1 - 1996/4/19

N2 - Recombinant human ribonuclease 1 (RNase 1) was chemically linked to recombinant human epidermal growth factor (EGF). The EGF-RNase conjugate showed dose-dependent cytotoxicity for EGF receptor-overexpressing A431 and TE-8 human squamous carcinoma cells with an IC50 of 2 x 10-7 M and 10-6 M, respectively, whereas the IC50 of RNase alone was almost 10-4M. An unconjugated mixture of EGF and RNase had no greater effect than RNase alone. The conjugate showed no detectable cytotoxicity against EGF receptor-deficient small cell lung cancer cells (H69). Addition to excess EGF in the medium protected A431 cells from the EGF-RNase conjugate cytotoxicity. The cytotoxicity of the EGF-RNase conjugate was positively correlated with the EGF receptor numbers of each cell line. The chimeric toxin composed of only human proteins might be a more useful anti-cancer agent with less immunogenicity than the conventional chimeric toxins.

AB - Recombinant human ribonuclease 1 (RNase 1) was chemically linked to recombinant human epidermal growth factor (EGF). The EGF-RNase conjugate showed dose-dependent cytotoxicity for EGF receptor-overexpressing A431 and TE-8 human squamous carcinoma cells with an IC50 of 2 x 10-7 M and 10-6 M, respectively, whereas the IC50 of RNase alone was almost 10-4M. An unconjugated mixture of EGF and RNase had no greater effect than RNase alone. The conjugate showed no detectable cytotoxicity against EGF receptor-deficient small cell lung cancer cells (H69). Addition to excess EGF in the medium protected A431 cells from the EGF-RNase conjugate cytotoxicity. The cytotoxicity of the EGF-RNase conjugate was positively correlated with the EGF receptor numbers of each cell line. The chimeric toxin composed of only human proteins might be a more useful anti-cancer agent with less immunogenicity than the conventional chimeric toxins.

KW - conjugate

KW - epidermal growth factor

KW - ribonuclease

KW - squamous carcinoma

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Epidermal growth factor receptor-dependent cytotoxicity for human squamous carcinoma cell lines of a conjugate composed of human EGF and RNase 1

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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